Cfd (NM_013459) Mouse Tagged ORF Clone

CAT#: MR225167

Cfd (Myc-DDK-tagged) - Mouse complement factor D (adipsin) (Cfd)

Product Images

Circular map for MR225167

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cfd
• Synonyms: A; Adn; DF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225167 representing NM_013459
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAGCTCCGTGTACTTCGTGGCTCTGGTGATCCTGGGAGCGGCTGTATGTGCAGCACAGCCCCGAG
  GCCGGATTCTGGGTGGCCAGGAGGCCGCAGCCCATGCTCGGCCCTACATGGCTTCCGTGCAAGTGAACGG
  CACACACGTGTGCGGTGGCACCCTGCTGGACGAGCAGTGGGTGCTCAGTGCTGCACACTGCATGGATGGA
  GTGACGGATGACGACTCTGTGCAGGTGCTCCTGGGTGCCCACTCCCTGTCCGCCCCTGAACCCTACAAGC
  GATGGTATGATGTGCAGAGTGTAGTGCCTCACCCGGGCAGCCGACCTGACAGCCTTGAGGACGACCTCAT
  TCTTTTTAAGCTATCCCAGAATGCCTCGTTGGGTCCCCACGTGAGACCCCTACCCTTGCAATACGAGGAC
  AAAGAAGTGGAACCCGGCACGCTCTGCGACGTGGCTGGTTGGGGTGTGGTCACCCATGCAGGACGCAGGC
  CTGATGTCCTGCATCAACTCAGAGTGTCAATCATGAACCGGACAACCTGCAATCTGCGCACGTACCATGA
  CGGGGTAGTCACCATTAACATGATGTGTGCAGAGAGCAACCGCAGGGACACTTGCAGGGGAGACTCCGGC
  AGCCCTCTAGTGTGCGGGGATGCAGTCGAAGGTGTGGTTACGTGGGGCTCTCGCGTCTGTGGCAATGGCA
  AAAAGCCGGGCGTCTATACCCGAGTGTCATCCTACCGGATGTGGATCGAAAACATCACAAATGGTAACAT
  GACATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013459
• ORF Size: 777 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013459.4
• RefSeq Size: 926 bp
• RefSeq ORF: 780 bp
• Locus ID: 11537
• UniProt ID: P03953
• Cytogenetics: 10 39.72 cM
• MW: 28.5 kDa
• Gene Summary: This gene encodes a serine protease that plays an important role in the alternative pathway of complement activation for pathogen recognition and elimination. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that in turn cleaves factor B in the complement pathway. This gene is expressed in adipocytes and the mature enzyme is secreted into the bloodstream. Mice lacking the encoded product cannot initiate alternative pathway of complement activation. [provided by RefSeq, Jul 2016]