Dysf (NM_021469) Mouse Tagged ORF Clone

CAT#: MR225131

Dysf (Myc-DDK-tagged) - Mouse dysferlin (Dysf), transcript variant 1

Product Images

Circular map for MR225131

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dysf
• Synonyms: 2310004N10Rik; AI604795; D6Pas3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225131 representing NM_021469
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTGTTGCTTGCTGGCAAGGGCCAGCAACCTCCCCAATGTGAAGAAGGACCGGCGCAGCGATCCAG
  TTGCCAGCCTGATTTTTCGAGGGGTAAAGAAGAGAACCAAAGTCATCAAGAACAGTGTGAACCCCGTGTG
  GAATGAGGGCTTTGAGTGGGACCTCAAAGGTATTCCTCTAGATCAGAGCTCAGAACTTCTCGTGGTGGTC
  AAGGACCATGAGACGATGGGAAGAAACAGGTTCCTGGGGGAAGCCAAGATCCCACTCCAGGAGGTCCTTG
  CCACCCCCAGCCTCTCTGCCAGCTTCAACGCACCTCTGCTAGACGCCAAGCAGCAACCCACGGGGGCCTC
  TCTGGTCCTGCAGGTGTCCTACACGCCACCCCCAGGAGCTGTGCCCCTGTTCCCACCACCTGCTTCTCTA
  GCACCCTCCCCGACACTGCCTGACATGGATCTGGTGCCTGGTGGGGGACAGAGCCGGGCTGAGACTTGGT
  CCCTGCTCAGTGACAGCACCATGGACACGAGATACTCTGGGAAGAAGTGGCCGGTCCCCACGGACACAGG
  AGGGGAGGAAGACACCGAGGACCAGGGCCTGACTGGAGATGAAGCTGAGCCGTTCCTGGACCAGAGTGCT
  GCTGTGGGCCCAGGGGGACCGACCACACCCAGGAAGCCACCTTCCCACCCTCCTCCCCACTATCCAGGGG
  CAAAAAGAAAAAGAAGCTCTGCCCCACCTAGAAAGCTGCTGTCAGACAAGCCACAGGACTTCCAGATCAG
  AGTCCAGGTGATAGAGGGGCGTCAGCTGCCTGGGGTGAATATTAAGCCTGTGGTCAAGGTCACAGCTGCT
  GGGCAGACCAAGCGAACTCGGATCCAGAAGGGAAATAGCCCACTCTTCAATGAGACTCTTTTCTTCAACG
  TATTTGACTCTCCCTTGGAGCTGTTTGATGAGCCCATCTTCATCACGGTGGTGGACTCACGCTCCCTTAG
  GACAGATGCCCTCCTTGGCGAGTTCCGGATGGATGTGGGCACCGTGTACAGAGAGCCCCGACACGCTTAT
  CTCCGGAAGTGGCTGCTGCTCTCCGACCCTGATGACTTCTCTGCGGGGGCCAGAGGCTACCTCAAAGCCA
  GCCTGTGTGTGCTAGGGCCTGGAGATGAAGCTCCCCTGGACAAGAAGGACCCTTCTGAAGACAAAGAGGA
  CATTGAAGGCAACCTGCTTAGGCCCACCGGTGTGGCCCTTCGAGGAGCCCACTTCTGCCTGAAGCTCTTC
  CGGGCTGAGGACTTACCACAGATGGACGATGCTGTGATGGACAACGTCAAGCAGATCTTTGGCTTTGACA
  GCAACAAGAAGAACTTGGTGGATCCCTTTGTGGAAGTCAGCTTTGCTGGGAAAATGCTCTGCAGCAAGAT
  CCTGGAGAAGACAGCTAACCCTCAGTGGAACCAGAACATCACCTTGCCTGCGATGTTTCCCTCTATGTGT
  GAAAAAATGAGGATTCGTGTCATGGACTGGGACCGCCTCACTCACAATGACACTGTGGCCACCACCTACT
  TGGGTATGTCGAAAATCTCTGCTACTGGAGGAGAAATAGAAGTGGACGACAACCTGGGCTTCCTCCCCAC
  TTTTGGGCCCTGCTATGTGAACCTCTATGGCAGTCCCCGAGAGTTCACGGGCTTCCCAGATCCTTATGCA
  GAGCTCAACACAGGAAAGGGAGAAGGTGTGGCTTATCGAGGCAGGGTTCTGTTGTCCCTGGAGACCAAAC
  TGGTGGAACACAGTGAGCAGAAGGTGGAAGACCTTCCTGCTGATGATATCCTGCGTGTGGAGAAGTACCT
  GCGGAGGCGCAAATATTCCCTCTTTGCTGCCTTCTACTCAGCCACAATGCTGCAGGATGTGGATGACGCC
  ATCCAGTTCGAGGTCAGCATTGGGAACTACGGGAACAAGTTCGACACCACCTGCCTGCCCTTGGCCTCCA
  CCACCCAGTACAGCCGGGCGGTCTTTGATGGATGCCACTACTATTACTTGCCTTGGGGCAACGTGAAGCC
  CGTGGTGGTGCTGTCCTCATACTGGGAAGACATCAGCCATCGAATTGAGATCCAAAACCAGCTCCTCAGG
  GTCGCTGACCGCCTGGAAGCTAACCTGGAGCAGGTCCACTTGGCTCTGAAGGCACAGTGTTCCTCCGAGG
  ATGTGGACGCCCTGGTGGCTCAGTTGACAGATGAGCTCCTTGCAGACTGCAGCCAGCCCCTGTGTGACAT
  CCACGAGATACCTTCTGCCACCCACCTTGATCAGTACCTGCTCCGGCTACGCACCCGTCACCTGAGCCAG
  ATCAAAGAAGCTGCTCTGGCCTTGAAGCTTGGCCATAGTGAGCTCTCCACTGCCCTGGAGCAGGCCGAGG
  ACTGGCTTCTGCATCTCCGTGCCCTGGCCGAGGAGCCCCAGAACAGCCTGCCGGACATCATCATCTGGAT
  GCTGCAGGGTGACAAGCGTGTGGCCTACCAGCGGGTGCCTGCCCATGAAGTCCTCTTCTCCCGGCGGGGC
  CCCAGCTACTGCGGCAGGAACTGTGGGAAGCTGCAGACCATCTTTCTGAAATATCCTATGGAAGGTATGC
  CTGGGGCCCGAATGCCCGTGCAGATTCGGATCAAGCTCTGGTTCGGGCTCTCTGTGGATGAGAAGGAATT
  CAATCAGTTTGCTGAGGGAAAGCTCTCTGTCTTTGCCGAAACGTATGAGAACCAGACAAAGCTGGCACTG
  GTCGGGAACTGGGGCACAACGGGCCTCACCTACCCCAAGTTTTCTGATGTCACAGGCAAGATCAAACTAC
  CAAAGGATAGCTTCCGCCCCTCAGCTGGCTGGGCCTGGGCAGGAGATTGGTTCGTGTGTCCAGAGAAGAC
  CCTACTCCATGATGCTGACGCTGGTCATCTGAGCTTCGTGGAGGAGGTGTTCGAGAACCAGACCCGGCTC
  CCTGGGGGCCAGTGGATTTACATGAGTGATAACTACACAGATGTGAATGGGGAGAAGGTACTTCCGAAGG
  ATGATATTGAATGCCCGCTGGGCTGGAAGTGGGAAGATGAAGAATGGTCCACAGACCTCAATCGGGCTGT
  TGATGAACAAGGCTGGGAGTACAGCATCACCATACCCCCAGACCGAAAGCCAAAACACTGGGTCCCTGTT
  GAGAAGATGTATTACACTCACAGGCGTCGACGCTGGGTCCGACTGCGCAGGAGGGACCTCAGCCAGATGG
  AAGCACTGAAGAGGCACAGGCAGGCAGAGGCCGAGGGCGAGGGCTGGGAGTATGCCTCTCTCTTCGGCTG
  GAAGTTCCACCTGGAGTACCGCAAGACAGATGCCTTCCGCCGCCGCCGCTGGCGCCGCCGCATGGAGCCG
  CTGGAGAAGACGGGACCTGCAGCTGTGTTTGCCCTCGAGGGGGCCCTGGGCGGCATGGTGGATGACAAGA
  GCGAAGACTCCATGTCTGTTTCTACCCTGAGCTTTGGCGTTAACAGACCCACAATCTCCTGCATCTTTGA
  CTATGGGAACCGCTACCATCTGCGCTGCTACCTGTACCAGGCCCGAGACCTGCCAGCCATGGACAAGGAC
  TCCTTCTCTGATCCCTACGCCATCGTCTCCTTCCTGCATCAAAGCCAGAAGACAGTGGTGGAGAAGAACA
  CCCTGAATCCCACCTGGGATCAAACCCTCATCTTCTATGAGATTGAGATCTTCGGCGAGCCAGCCAGTAT
  CGCCGAGCATCCGCCCTGCATCGTGGTGGAGCTCTATGACCACGACACCTATGGTGCTGACGAGTTCATG
  GGTCGCTGTATTTGTCAGCCAAGCCTGGAACGGATGCCCCGCCTGGCCTGGTTCCCACTGACCCGGGGCA
  GCCAGCCTGCCGGCGAGCTGCTGGCAGCGTTCGAGCTTATCCAGAGAGAGAAGCCAGCCATCCATCACAT
  CCCTGGCTTTGAGATGCATGAAACTTCAAGGATTTTGGATGAGACGGAGGACACAGATTTGCCTTACCCA
  CCACCTCAAAGGGAGGCCAACATCTACATGGTTCCCCAGAACATTAAGCCAGCTCTCCAACGTACTGCCA
  TTGAGATCCTGGCCTGGGGCCTGCGGAACATGAAGAGCTATCAGATGGCCAGCATCTCTTCCCCCAGCCT
  CGTGGTGGAGTGTGGTGGGCAGACAGTGCAGTCCTGTGTCATCAGAAACCTTCGGAAGAACCCCAACTTT
  GATGTCTGTACTCTCTTCATGGAAGTGATGTTACCCAGAGAGGACCTCTACTGTCCACCTATTGTGGTCA
  AGGTCATTGATAATCGCCAGTTTGGCCGCCGGCCAGTTGTGGGTCAGTGTACCATTCGCTCCCTGGAGAA
  CTTCCTGTGCGACCCATACTCAGCAGAGAGTCCATCCCCACAGGGTGGCCCAGATGATGTGAGCTTACTC
  AGCCCCGGGGAAGATGTGCTGATTGACATCGATGACAAGGAGCCTCTCATTCCTGTCCAGGAGGAGGAGT
  TCATTGACTGGTGGAGCAAATTCTTTGCTTCTGTCGGGGAGAGGGAAAAGTGTGGTTCCTACCTGGAGAA
  GGATTTTGACACCTTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCTTTTGGAGGCCTGTCCGAC
  TTTTGTAACACCTTCAAGCTCTACCGGGGCAGGACTCAGGAGGAGACAGATGATCCATCTGTCATCGGAG
  AATTTAAGGGTCTCTTCAAAATTTATCCCCTCCCAGAAGATCCAGCCATCCCCATGCCCCCGAGACAATT
  CCATCAGCTGGCTGCTCAGGGTCCCCAGGAATGCTTGGTCCGTATTTATATCGTCCGAGCATTTGGCTTA
  CAGCCCAAGGACCCCAATGGCAAGTGTGACCCTTACATCAAGATCTCCATAGGGAAGAAATCAGTGAGTG
  ACCAGGATAACTACATCCCTTGCACCCTGGAGCCTGTATTCGGAAAGATGTTTGAACTCACCTGCACTCT
  ACCTCTGGAGAAGGACCTGAAGATCACACTCTACGATTATGACCTGCTCTCCAAGGATGAGAAGATTGGG
  GAGACGGTCATAGACCTGGAGAACAGGCTGCTGTCCAAGTTTGGGGCTCGCTGTGGGCTCCCACAGACCT
  ACTGTGTCTCTGGACCAAATCAGTGGAGAGATCAGCTCCGCCCCTCCCAGCTCCTCCACCTCTTCTGCCA
  GCAGCACAGGATCAAGGCCCCCGTGTACCGGACAGACCGAGTGACGTTTCAGGATAAGGACTACACCATT
  GAGGAGATAGAGGCTGGCAGACTCCCAAACCCACACCTGGGCCCAGTGGAGGAACGCTTAGCCCTGCATG
  TCCTTCAGCAACAAGGCTTGGTTCCTGAGCATGTGGAGTCACGGCCTCTTTATAGTCCTCTGCAGCCAGA
  TATCGAGCAGGGGAAGCTACAGATGTGGATTGACATATTTCCAAAGGTGCTGGGCCGGCCTGGACCTCCC
  TTCAACATCACCCCACGGAAAGCTAGAAGGTTTTTTCTGCGTTGTATTATTTGGAACACAAAAGATGTGA
  TCTTGGATGACCTCAGTCTCACGGGGGAGAAGATGAGTGACATCTATGTGAAAGGCTGGATGGTGGGATT
  CGAAGAACACAAGCAGAAGACAGATGTGCACTACCGCTCCCTGGGTGGGGAGGGCAATTTCAACTGGAGG
  TTCGTGTTTCCCTTCGACTATCTGCCTGCTGAGCAAGTCTGTGCTGTTGCCAAGAAGGATGCCTTCTGGA
  GACTCGACAAGACAGAGAGCAAGATCCCAGCACGTGTGGTCTTCCAGATCTGGGACAATGACAAATTCTC
  TTTTGATGACTTTCTGGGCTCTCTGCAGCTGGATCTCAACCGAATGCCCAAGCCAGCCAAGACGGCTGAG
  AAGTGCTCCTTGGACCAGCTGGATGACACCTTCCACCCAGAATGGTTTGTGTCCCTTTTTGAGCAGAAGA
  CAGTGAAAGGATGGTGGCCTTGTGTGACAGAGGAGGGCGAGAAGAAGATGTTGGCGGGCAAGCTGGAAAT
  GACCTTGGAGATTGTTGCAGAAAGTGAACATGAAGAGCGGCCTGCTGGCCAAGGTCGGGATGAACCCAAC
  ATGAATCCGAAGCTAGAGGATCCAAGGCGCCCCGATACTTCTTTCCTGTGGTTCACCTCCCCGTACAAGA
  CCATGAAGTTCATCTTGTGGCGACGCTTCCGGTGTGCCATCATCCTCTTCATCATCCTCTTCATCCTCCT
  GCTCTTCCTGGGTGTCTTTGTCTACGCCTTCCCGAACTACGCTGCCATGAAGCTGGTGAAGCCCTTTCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225131 representing NM_021469
  Red=Cloning site Green=Tags(s)
  
  MLCCLLARASNLPNVKKDRRSDPVASLIFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELLVVV
  KDHETMGRNRFLGEAKIPLQEVLATPSLSASFNAPLLDAKQQPTGASLVLQVSYTPPPGAVPLFPPPASL
  APSPTLPDMDLVPGGGQSRAETWSLLSDSTMDTRYSGKKWPVPTDTGGEEDTEDQGLTGDEAEPFLDQSA
  AVGPGGPTTPRKPPSHPPPHYPGAKRKRSSAPPRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAA
  GQTKRTRIQKGNSPLFNETLFFNVFDSPLELFDEPIFITVVDSRSLRTDALLGEFRMDVGTVYREPRHAY
  LRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLDKKDPSEDKEDIEGNLLRPTGVALRGAHFCLKLF
  RAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMC
  EKMRIRVMDWDRLTHNDTVATTYLGMSKISATGGEIEVDDNLGFLPTFGPCYVNLYGSPREFTGFPDPYA
  ELNTGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDA
  IQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIEIQNQLLR
  VADRLEANLEQVHLALKAQCSSEDVDALVAQLTDELLADCSQPLCDIHEIPSATHLDQYLLRLRTRHLSQ
  IKEAALALKLGHSELSTALEQAEDWLLHLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHEVLFSRRG
  PSYCGRNCGKLQTIFLKYPMEGMPGARMPVQIRIKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLAL
  VGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWAWAGDWFVCPEKTLLHDADAGHLSFVEEVFENQTRL
  PGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPV
  EKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEP
  LEKTGPAAVFALEGALGGMVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYLYQARDLPAMDKD
  SFSDPYAIVSFLHQSQKTVVEKNTLNPTWDQTLIFYEIEIFGEPASIAEHPPCIVVELYDHDTYGADEFM
  GRCICQPSLERMPRLAWFPLTRGSQPAGELLAAFELIQREKPAIHHIPGFEMHETSRILDETEDTDLPYP
  PPQREANIYMVPQNIKPALQRTAIEILAWGLRNMKSYQMASISSPSLVVECGGQTVQSCVIRNLRKNPNF
  DVCTLFMEVMLPREDLYCPPIVVKVIDNRQFGRRPVVGQCTIRSLENFLCDPYSAESPSPQGGPDDVSLL
  SPGEDVLIDIDDKEPLIPVQEEEFIDWWSKFFASVGEREKCGSYLEKDFDTLKVYDTQLENVEAFGGLSD
  FCNTFKLYRGRTQEETDDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGL
  QPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIG
  ETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRIKAPVYRTDRVTFQDKDYTI
  EEIEAGRLPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDIFPKVLGRPGPP
  FNITPRKARRFFLRCIIWNTKDVILDDLSLTGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWR
  FVFPFDYLPAEQVCAVAKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAE
  KCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVTEEGEKKMLAGKLEMTLEIVAESEHEERPAGQGRDEPN
  MNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGVFVYAFPNYAAMKLVKPFR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021469
• ORF Size: 6300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021469.3, NP_067444.2
• RefSeq Size: 6643 bp
• RefSeq ORF: 6303 bp
• Locus ID: 26903
• UniProt ID: Q9ESD7
• Cytogenetics: 6 36.14 cM
• MW: 239.7 kDa
• Gene Summary: Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.[UniProtKB/Swiss-Prot Function]