Dysf (NM_021469) Mouse Untagged Clone

CAT#: MC225170

Dysf (untagged) - Mouse dysferlin (Dysf), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dysf
• Synonyms: 2310004N10Rik; AI604795; D6Pas3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225170 representing NM_021469
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTGTTGCTTGCTGGCAAGGGCCAGCAACCTCCCCAATGTGAAGAAGGACCGGCGCAGCGATCCAG
  TTGCCAGCCTGATTTTTCGAGGGGTAAAGAAGAGAACCAAAGTCATCAAGAACAGTGTGAACCCCGTGTG
  GAATGAGGGCTTTGAGTGGGACCTCAAAGGTATTCCTCTAGATCAGAGCTCAGAACTTCTCGTGGTGGTC
  AAGGACCATGAGACGATGGGAAGAAACAGGTTCCTGGGGGAAGCCAAGATCCCACTCCAGGAGGTCCTTG
  CCACCCCCAGCCTCTCTGCCAGCTTCAACGCACCTCTGCTAGACGCCAAGCAGCAACCCACGGGGGCCTC
  TCTGGTCCTGCAGGTGTCCTACACGCCACCCCCAGGAGCTGTGCCCCTGTTCCCACCACCTGCTTCTCTA
  GCACCCTCCCCGACACTGCCTGACATGGATCTGGTGCCTGGTGGGGGACAGAGCCGGGCTGAGACTTGGT
  CCCTGCTCAGTGACAGCACCATGGACACGAGATACTCTGGGAAGAAGTGGCCGGTCCCCACGGACACAGG
  AGGGGAGGAAGACACCGAGGACCAGGGCCTGACTGGAGATGAAGCTGAGCCGTTCCTGGACCAGAGTGCT
  GCTGTGGGCCCAGGGGGACCGACCACACCCAGGAAGCCACCTTCCCACCCTCCTCCCCACTATCCAGGGG
  CAAAAAGAAAAAGAAGCTCTGCCCCACCTAGAAAGCTGCTGTCAGACAAGCCACAGGACTTCCAGATCAG
  AGTCCAGGTGATAGAGGGGCGTCAGCTGCCTGGGGTGAATATTAAGCCTGTGGTCAAGGTCACAGCTGCT
  GGGCAGACCAAGCGAACTCGGATCCAGAAGGGAAATAGCCCACTCTTCAATGAGACTCTTTTCTTCAACG
  TATTTGACTCTCCCTTGGAGCTGTTTGATGAGCCCATCTTCATCACGGTGGTGGACTCACGCTCCCTTAG
  GACAGATGCCCTCCTTGGCGAGTTCCGGATGGATGTGGGCACCGTGTACAGAGAGCCCCGACACGCTTAT
  CTCCGGAAGTGGCTGCTGCTCTCCGACCCTGATGACTTCTCTGCGGGGGCCAGAGGCTACCTCAAAGCCA
  GCCTGTGTGTGCTAGGGCCTGGAGATGAAGCTCCCCTGGACAAGAAGGACCCTTCTGAAGACAAAGAGGA
  CATTGAAGGCAACCTGCTTAGGCCCACCGGTGTGGCCCTTCGAGGAGCCCACTTCTGCCTGAAGCTCTTC
  CGGGCTGAGGACTTACCACAGATGGACGATGCTGTGATGGACAACGTCAAGCAGATCTTTGGCTTTGACA
  GCAACAAGAAGAACTTGGTGGATCCCTTTGTGGAAGTCAGCTTTGCTGGGAAAATGCTCTGCAGCAAGAT
  CCTGGAGAAGACAGCTAACCCTCAGTGGAACCAGAACATCACCTTGCCTGCGATGTTTCCCTCTATGTGT
  GAAAAAATGAGGATTCGTGTCATGGACTGGGACCGCCTCACTCACAATGACACTGTGGCCACCACCTACT
  TGGGTATGTCGAAAATCTCTGCTACTGGAGGAGAAATAGAAGTGGACGACAACCTGGGCTTCCTCCCCAC
  TTTTGGGCCCTGCTATGTGAACCTCTATGGCAGTCCCCGAGAGTTCACGGGCTTCCCAGATCCTTATGCA
  GAGCTCAACACAGGAAAGGGAGAAGGTGTGGCTTATCGAGGCAGGGTTCTGTTGTCCCTGGAGACCAAAC
  TGGTGGAACACAGTGAGCAGAAGGTGGAAGACCTTCCTGCTGATGATATCCTGCGTGTGGAGAAGTACCT
  GCGGAGGCGCAAATATTCCCTCTTTGCTGCCTTCTACTCAGCCACAATGCTGCAGGATGTGGATGACGCC
  ATCCAGTTCGAGGTCAGCATTGGGAACTACGGGAACAAGTTCGACACCACCTGCCTGCCCTTGGCCTCCA
  CCACCCAGTACAGCCGGGCGGTCTTTGATGGATGCCACTACTATTACTTGCCTTGGGGCAACGTGAAGCC
  CGTGGTGGTGCTGTCCTCATACTGGGAAGACATCAGCCATCGAATTGAGATCCAAAACCAGCTCCTCAGG
  GTCGCTGACCGCCTGGAAGCTAACCTGGAGCAGGTCCACTTGGCTCTGAAGGCACAGTGTTCCTCCGAGG
  ATGTGGACGCCCTGGTGGCTCAGTTGACAGATGAGCTCCTTGCAGACTGCAGCCAGCCCCTGTGTGACAT
  CCACGAGATACCTTCTGCCACCCACCTTGATCAGTACCTGCTCCGGCTACGCACCCGTCACCTGAGCCAG
  ATCAAAGAAGCTGCTCTGGCCTTGAAGCTTGGCCATAGTGAGCTCTCCACTGCCCTGGAGCAGGCCGAGG
  ACTGGCTTCTGCATCTCCGTGCCCTGGCCGAGGAGCCCCAGAACAGCCTGCCGGACATCATCATCTGGAT
  GCTGCAGGGTGACAAGCGTGTGGCCTACCAGCGGGTGCCTGCCCATGAAGTCCTCTTCTCCCGGCGGGGC
  CCCAGCTACTGCGGCAGGAACTGTGGGAAGCTGCAGACCATCTTTCTGAAATATCCTATGGAAGGTATGC
  CTGGGGCCCGAATGCCCGTGCAGATTCGGATCAAGCTCTGGTTCGGGCTCTCTGTGGATGAGAAGGAATT
  CAATCAGTTTGCTGAGGGAAAGCTCTCTGTCTTTGCCGAAACGTATGAGAACCAGACAAAGCTGGCACTG
  GTCGGGAACTGGGGCACAACGGGCCTCACCTACCCCAAGTTTTCTGATGTCACAGGCAAGATCAAACTAC
  CAAAGGATAGCTTCCGCCCCTCAGCTGGCTGGGCCTGGGCAGGAGATTGGTTCGTGTGTCCAGAGAAGAC
  CCTACTCCATGATGCTGACGCTGGTCATCTGAGCTTCGTGGAGGAGGTGTTCGAGAACCAGACCCGGCTC
  CCTGGGGGCCAGTGGATTTACATGAGTGATAACTACACAGATGTGAATGGGGAGAAGGTACTTCCGAAGG
  ATGATATTGAATGCCCGCTGGGCTGGAAGTGGGAAGATGAAGAATGGTCCACAGACCTCAATCGGGCTGT
  TGATGAACAAGGCTGGGAGTACAGCATCACCATACCCCCAGACCGAAAGCCAAAACACTGGGTCCCTGTT
  GAGAAGATGTATTACACTCACAGGCGTCGACGCTGGGTCCGACTGCGCAGGAGGGACCTCAGCCAGATGG
  AAGCACTGAAGAGGCACAGGCAGGCAGAGGCCGAGGGCGAGGGCTGGGAGTATGCCTCTCTCTTCGGCTG
  GAAGTTCCACCTGGAGTACCGCAAGACAGATGCCTTCCGCCGCCGCCGCTGGCGCCGCCGCATGGAGCCG
  CTGGAGAAGACGGGACCTGCAGCTGTGTTTGCCCTCGAGGGGGCCCTGGGCGGCATGGTGGATGACAAGA
  GCGAAGACTCCATGTCTGTTTCTACCCTGAGCTTTGGCGTTAACAGACCCACAATCTCCTGCATCTTTGA
  CTATGGGAACCGCTACCATCTGCGCTGCTACCTGTACCAGGCCCGAGACCTGCCAGCCATGGACAAGGAC
  TCCTTCTCTGATCCCTACGCCATCGTCTCCTTCCTGCATCAAAGCCAGAAGACAGTGGTGGAGAAGAACA
  CCCTGAATCCCACCTGGGATCAAACCCTCATCTTCTATGAGATTGAGATCTTCGGCGAGCCAGCCAGTAT
  CGCCGAGCATCCGCCCTGCATCGTGGTGGAGCTCTATGACCACGACACCTATGGTGCTGACGAGTTCATG
  GGTCGCTGTATTTGTCAGCCAAGCCTGGAACGGATGCCCCGCCTGGCCTGGTTCCCACTGACCCGGGGCA
  GCCAGCCTGCCGGCGAGCTGCTGGCAGCGTTCGAGCTTATCCAGAGAGAGAAGCCAGCCATCCATCACAT
  CCCTGGCTTTGAGATGCATGAAACTTCAAGGATTTTGGATGAGACGGAGGACACAGATTTGCCTTACCCA
  CCACCTCAAAGGGAGGCCAACATCTACATGGTTCCCCAGAACATTAAGCCAGCTCTCCAACGTACTGCCA
  TTGAGATCCTGGCCTGGGGCCTGCGGAACATGAAGAGCTATCAGATGGCCAGCATCTCTTCCCCCAGCCT
  CGTGGTGGAGTGTGGTGGGCAGACAGTGCAGTCCTGTGTCATCAGAAACCTTCGGAAGAACCCCAACTTT
  GATGTCTGTACTCTCTTCATGGAAGTGATGTTACCCAGAGAGGACCTCTACTGTCCACCTATTGTGGTCA
  AGGTCATTGATAATCGCCAGTTTGGCCGCCGGCCAGTTGTGGGTCAGTGTACCATTCGCTCCCTGGAGAA
  CTTCCTGTGCGACCCATACTCAGCAGAGAGTCCATCCCCACAGGGTGGCCCAGATGATGTGAGCTTACTC
  AGCCCCGGGGAAGATGTGCTGATTGACATCGATGACAAGGAGCCTCTCATTCCTGTCCAGGAGGAGGAGT
  TCATTGACTGGTGGAGCAAATTCTTTGCTTCTGTCGGGGAGAGGGAAAAGTGTGGTTCCTACCTGGAGAA
  GGATTTTGACACCTTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCTTTTGGAGGCCTGTCCGAC
  TTTTGTAACACCTTCAAGCTCTACCGGGGCAGGACTCAGGAGGAGACAGATGATCCATCTGTCATCGGAG
  AATTTAAGGGTCTCTTCAAAATTTATCCCCTCCCAGAAGATCCAGCCATCCCCATGCCCCCGAGACAATT
  CCATCAGCTGGCTGCTCAGGGTCCCCAGGAATGCTTGGTCCGTATTTATATCGTCCGAGCATTTGGCTTA
  CAGCCCAAGGACCCCAATGGCAAGTGTGACCCTTACATCAAGATCTCCATAGGGAAGAAATCAGTGAGTG
  ACCAGGATAACTACATCCCTTGCACCCTGGAGCCTGTATTCGGAAAGATGTTTGAACTCACCTGCACTCT
  ACCTCTGGAGAAGGACCTGAAGATCACACTCTACGATTATGACCTGCTCTCCAAGGATGAGAAGATTGGG
  GAGACGGTCATAGACCTGGAGAACAGGCTGCTGTCCAAGTTTGGGGCTCGCTGTGGGCTCCCACAGACCT
  ACTGTGTCTCTGGACCAAATCAGTGGAGAGATCAGCTCCGCCCCTCCCAGCTCCTCCACCTCTTCTGCCA
  GCAGCACAGGATCAAGGCCCCCGTGTACCGGACAGACCGAGTGACGTTTCAGGATAAGGACTACACCATT
  GAGGAGATAGAGGCTGGCAGACTCCCAAACCCACACCTGGGCCCAGTGGAGGAACGCTTAGCCCTGCATG
  TCCTTCAGCAACAAGGCTTGGTTCCTGAGCATGTGGAGTCACGGCCTCTTTATAGTCCTCTGCAGCCAGA
  TATCGAGCAGGGGAAGCTACAGATGTGGATTGACATATTTCCAAAGGTGCTGGGCCGGCCTGGACCTCCC
  TTCAACATCACCCCACGGAAAGCTAGAAGGTTTTTTCTGCGTTGTATTATTTGGAACACAAAAGATGTGA
  TCTTGGATGACCTCAGTCTCACGGGGGAGAAGATGAGTGACATCTATGTGAAAGGCTGGATGGTGGGATT
  CGAAGAACACAAGCAGAAGACAGATGTGCACTACCGCTCCCTGGGTGGGGAGGGCAATTTCAACTGGAGG
  TTCGTGTTTCCCTTCGACTATCTGCCTGCTGAGCAAGTCTGTGCTGTTGCCAAGAAGGATGCCTTCTGGA
  GACTCGACAAGACAGAGAGCAAGATCCCAGCACGTGTGGTCTTCCAGATCTGGGACAATGACAAATTCTC
  TTTTGATGACTTTCTGGGCTCTCTGCAGCTGGATCTCAACCGAATGCCCAAGCCAGCCAAGACGGCTGAG
  AAGTGCTCCTTGGACCAGCTGGATGACACCTTCCACCCAGAATGGTTTGTGTCCCTTTTTGAGCAGAAGA
  CAGTGAAAGGATGGTGGCCTTGTGTGACAGAGGAGGGCGAGAAGAAGATGTTGGCGGGCAAGCTGGAAAT
  GACCTTGGAGATTGTTGCAGAAAGTGAACATGAAGAGCGGCCTGCTGGCCAAGGTCGGGATGAACCCAAC
  ATGAATCCGAAGCTAGAGGATCCAAGGCGCCCCGATACTTCTTTCCTGTGGTTCACCTCCCCGTACAAGA
  CCATGAAGTTCATCTTGTGGCGACGCTTCCGGTGTGCCATCATCCTCTTCATCATCCTCTTCATCCTCCT
  GCTCTTCCTGGGTGTCTTTGTCTACGCCTTCCCGAACTACGCTGCCATGAAGCTGGTGAAGCCCTTTCGA
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021469
• Insert Size: 6303 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021469.3, NP_067444.2
• RefSeq Size: 6663 bp
• RefSeq ORF: 6303 bp
• Locus ID: 26903
• UniProt ID: Q9ESD7
• Cytogenetics: 6 36.14 cM
• Gene Summary: Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).