Ofd1 (NM_177429) Mouse Tagged ORF Clone

CAT#: MR225128

Ofd1 (Myc-DDK-tagged) - Mouse oral-facial-digital syndrome 1 gene homolog (human) (Ofd1)

Product Images

Circular map for MR225128

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ofd1
• Synonyms: Cxorf5; DXGgc7e; ORF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225128 representing NM_177429
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATGGCTCAGTCCAACATGCCCCACAAGTCTGATGTGTTGAGTCAAGATGAACTGCGCAAAAAGC
  TATACCAGACGTTTAAGGATCGGGGTGTACTGGACACACTTCAGACCCAACTCCGAAACCAGCTAATTCA
  TGAATTGATGCATCCTGTATTGAGTGGAGAAGTGAAGCCGCCATCCATTTCAGTGGAAGGGAGTGCCCTC
  CTAATAGGGGCTTCTAACTCGCTGGTGGCAGACCACTTACAAAGATGTGGCTATGAATATTCACTCTCTG
  TTTTCTTTCCAGAAAGTGGTTTGGCAAAAGAAAAGATATTTACTATGCAGGATCTACTACAACTCATTCG
  GATTAACCCCTCATCCAGTCTCTATAAATCATTGATTTCAGGATTTGATAAAGAAAATAAAAAAGGTTTT
  CTTATGAGTTTCTTAAAGGAGTTGGCAGAATATTATCAAGCTAAAGAGAGCTGTGATGCGGAAACTCAGA
  CAAGCACCACATTTCCCAGCCAAGTGTCCCTAGCCGAGAAGTTTCAGCTTATTGACGCTCAATTTGCAGA
  TGGTTTTCCTCACCGTTCCAAGCTAGAATCTTTAGAGACAAAGTTAAACGAGTATAAGAAAGAAGTACAA
  CACCAGCTTCAGGTAGAAATGTGTCATAAGCTGAAGTATTTTAGAGAAGCTGAAATAACAAAAGTTAAGA
  TGGAAGAGAGAAGAAAGTATGAAAAGGAATTGGCTGAGTTCCAGAATGAATTTGAAAGAACTTGTCAAGC
  AAAAAATGAAGCCCTCATTTCTCAGGAGAAGAATTCTCTGGAAAGAATTAAAAAACACCGAGAGATGGAA
  TCAAAAGAAATTTATGCTCAAAGACAGCTTCTACTAAATGATATAGCTTTGCTTAGAGGTAGAGAAGCAG
  AGCTGAAGGAGCGAATCGAAACTTTTGAATTGACCCAGAAGCTCCAAGAAGAAAAAATTAAAAGTGAGGC
  TGAGGCACTTGAGAGACGGGAACAGAATCTGAAGAATATTGAAGACACCTATGATCAGAAACTCAAAACT
  GAACTGCTAAAGTACCAACTGGAGCTAAAGGATGACTACATCACTAGGACAAATAAACTCCTGGAAGAGG
  AAAGGAAGAATAAAGAAAAAACTATTCATTTGCAAGAGGAGCTCACAGTTATTAATTCAAAAAAGGAAGA
  ACTCAGTAAATCTGTTAAGCATATGAAAGAAGTTGAGCTGGAACTGGAGTCTGTGAAAGCTCAGTTTTTA
  GCAATATCAAAACAAAACCACTTGCTGAATGAAAAGGTGAGAGAGATGAGTGATTACTCACAACTGAAAG
  AAGAGAAAGTGGAGCTTCAGGCACAAAATAAACTACTTAAACTGCAACTGGAAGAGACTAGGAATGAAAA
  CTTACGACTCCTAGACCGCATAACTCAGCCACCTCCTGAGCTTGTGATTTTTCAGAAGGAATTACAGAAA
  ACAGAAAAGGCTATGGAACTGGAGCACAAGGACTTTGAAACTCATAGGCAGGCTCTTGAGAAACAGCTGC
  AGAGTGAAATTGAAAATTCTGCACAGCTAAGGACCCAGATAGCAGAATATGATGCTTCTGTCAAAAGGTT
  AACTGTTCAGGTGGCTGAGTTAAAGTCACAACTAAAACAAACTCAGATAGCCCTAGAGAATGAAGTGTAC
  CGAAATCCAAAACACTCTCTGATTCATTCCCTGAGTGGCCTCCTATTGAGCGGCAAGATGGCACCCCACA
  GTGAAGATAAAAGTGGGGATTTCTTGAATGTACCCCTTGAACAGAACAAGGTTATAGCAGGTGCAGTTAT
  GTCAAGGGTCCCACCTTATGTAAATACAGCAACAGAAGCTAGTTCCCCAGAGTCTGACTTTGAGTTTATA
  GCCAGTAGTACTAAAGCCAAAGTAAGAGAGCTAGAGCAAGAGGCTGAACGCTTGGAAAAGGCATTCAGAA
  CTTACTATCAAAGAGCTACTCAGAATCCTTCTACGAGCCCTCAGCCGGCAAAGAGTCCTCCATCTGTAAA
  CTCGGTGGCAGCTCTCAGAAGCATAGCTTCCAGTTCCATGGACAGACCTGTTTCTGCAGAGGACAGAGTT
  GTCTCGGAACAGCCTCTTGGGGACATGCTGAAGGAAGAAATGAGTGATATGTCCAAAGCATTCATGGGCA
  GTGTGGTTTCACGGCCACGCAGGACCTCTTCCTCCACACGCCTCTCCTCTACACCCCATCCAAAATCGAG
  AAGAAGTCTTGATAATGAAATGTATCTGGAAGGTCTCGGGAGATTACACATGACTTCCTCCAGCCCTCTT
  CTTGACAGAGTTTCTGCATCACCTGCTGCTTCCCCTAGTCCTTGTCCTGAGAGAACAGCCCAGGCGTCAC
  CTGTTCCATCTAGGCACAGCTTCTCTGGTCTTCCAGAGCAAAATGCCTGTTTATATCAGAGACAAACTGA
  AACGCAAGATAAAAGTGAGCTTTCAAATGTGGACAAGCAATCTTTGAAAGATGAGAAATTTGAACCACCT
  TTCAGATGGAACAAGACAGAACAGTTTGAAGCAGAAGGACTCCACCCTGCTGGTGACATGCCTGGAATAG
  ATTTTGCTGTGGCTACTCAGTCTTCAAGACTCATTTCATACGACTACCCAAGTGCTGTTCAGAGCCAAAC
  TGGAGAACAAGACGAACAGGAGCTATGGGAGCTTCACATGAAAGAACGCAGGCAGAGAGAAGAACAAAGG
  CATAATGAACGGCAAGAAGCTTTAGAGAGGGAAAGAAGAGAGCTGGGAAAACTAGAGCAGGAGCGGAGGA
  TGATTGAAGAGTCACTGAAGATGGAGATGGAAGAAGAATTAGAAAAGAGTGTTCAAGACCAGAAAGACAA
  ATCAGCTCATTGTGAAAATACTTTAGAAAAATACATGAAAATTATCCAGCAGAGACAGGAAGAATCAAAT
  GCAGATAAGAGCTCCAAAAAATCGGGCAAAGAATGCTCACTAGTGGACATGATGATGCCTAGTGACAAGG
  ATGAAAGTTCTCCAGGATTTTCTCATGAAGAACCAGATGACATGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225128 representing NM_177429
  Red=Cloning site Green=Tags(s)
  
  MRMAQSNMPHKSDVLSQDELRKKLYQTFKDRGVLDTLQTQLRNQLIHELMHPVLSGEVKPPSISVEGSAL
  LIGASNSLVADHLQRCGYEYSLSVFFPESGLAKEKIFTMQDLLQLIRINPSSSLYKSLISGFDKENKKGF
  LMSFLKELAEYYQAKESCDAETQTSTTFPSQVSLAEKFQLIDAQFADGFPHRSKLESLETKLNEYKKEVQ
  HQLQVEMCHKLKYFREAEITKVKMEERRKYEKELAEFQNEFERTCQAKNEALISQEKNSLERIKKHREME
  SKEIYAQRQLLLNDIALLRGREAELKERIETFELTQKLQEEKIKSEAEALERREQNLKNIEDTYDQKLKT
  ELLKYQLELKDDYITRTNKLLEEERKNKEKTIHLQEELTVINSKKEELSKSVKHMKEVELELESVKAQFL
  AISKQNHLLNEKVREMSDYSQLKEEKVELQAQNKLLKLQLEETRNENLRLLDRITQPPPELVIFQKELQK
  TEKAMELEHKDFETHRQALEKQLQSEIENSAQLRTQIAEYDASVKRLTVQVAELKSQLKQTQIALENEVY
  RNPKHSLIHSLSGLLLSGKMAPHSEDKSGDFLNVPLEQNKVIAGAVMSRVPPYVNTATEASSPESDFEFI
  ASSTKAKVRELEQEAERLEKAFRTYYQRATQNPSTSPQPAKSPPSVNSVAALRSIASSSMDRPVSAEDRV
  VSEQPLGDMLKEEMSDMSKAFMGSVVSRPRRTSSSTRLSSTPHPKSRRSLDNEMYLEGLGRLHMTSSSPL
  LDRVSASPAASPSPCPERTAQASPVPSRHSFSGLPEQNACLYQRQTETQDKSELSNVDKQSLKDEKFEPP
  FRWNKTEQFEAEGLHPAGDMPGIDFAVATQSSRLISYDYPSAVQSQTGEQDEQELWELHMKERRQREEQR
  HNERQEALERERRELGKLEQERRMIEESLKMEMEEELEKSVQDQKDKSAHCENTLEKYMKIIQQRQEESN
  ADKSSKKSGKECSLVDMMMPSDKDESSPGFSHEEPDDMW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177429
• ORF Size: 3057 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177429.3, NP_803178.2
• RefSeq Size: 4453 bp
• RefSeq ORF: 3060 bp
• Locus ID: 237222
• UniProt ID: Q80Z25
• Cytogenetics: X 77.28 cM
• MW: 118.1 kDa
• Gene Summary: Component of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis. Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation.[UniProtKB/Swiss-Prot Function]