Taar1 (NM_053205) Mouse Tagged ORF Clone

CAT#: MR225090

Taar1 (Myc-DDK-tagged) - Mouse trace amine-associated receptor 1 (Taar1)

Product Images

Circular map for MR225090

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Taar1
• Synonyms: taR-1; Tar1; Trar1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225090 representing NM_053205
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTTTGCCACGCTATCACAAACATTTCCCACAGAAACAGCGACTGGTCAAGAGAAGTCCAAGCTT
  CCCTGTACAGCTTAATGTCACTCATAATCCTGGCCACTCTGGTTGGCAACTTAATAGTAATAATTTCCAT
  ATCCCATTTCAAGCAACTTCATACACCCACCAACTGGCTCCTTCACTCCATGGCCATTGTCGACTTTCTG
  CTGGGCTGTCTGATAATGCCCTGCAGCATGGTGAGAACTGTTGAGCGCTGTTGGTATTTTGGGGAAATCC
  TCTGTAAAGTTCACACCAGCACCGATATCATGCTGAGCTCCGCCTCCATTTTCCACTTAGCTTTCATTTC
  CATTGACCGCTACTGTGCTGTGTGTGACCCTTTGAGATACAAAGCCAAGATCAATATCTCCACTATTCTT
  GTGATGATCCTCGTTAGTTGGAGCCTTCCTGCTGTTTATGCATTTGGGATGATCTTCCTGGAACTGAACT
  TAAAAGGAGTGGAAGAGCTGTATCGCAGTCAGGTCAGCGACCTGGGCGGCTGTTCTCCCTTCTTTAGTAA
  AGTATCTGGGGTACTGGCGTTCATGACTTCCTTCTATATACCTGGATCTGTTATGTTATTTGTTTACTAT
  AGGATATATTTCATAGCTAAAGGACAAGCAAGGTCAATCAATCGTACGAATGTTCAAGTTGGATTGGAAG
  GGAAAAGCCAAGCACCACAAAGCAAGGAAACAAAAGCCGCGAAGACCTTAGGGATCATGGTGGGCGTTTT
  CCTCGTATGCTGGTGCCCGTTCTTTCTCTGCACGGTCCTGGACCCTTTCCTGGGCTATGTTATCCCACCC
  TCTCTGAATGACGCACTGTATTGGTTTGGGTACTTGAATTCTGCCCTCAATCCGATGGTTTATGCCTTTT
  TCTATCCCTGGTTCAGAAGAGCCTTGAAGATGGTTCTCCTTGGTAAAATTTTCCAAAAAGATTCATCTAG
  GTCTAAGCTATTTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225090 representing NM_053205
  Red=Cloning site Green=Tags(s)
  
  MHLCHAITNISHRNSDWSREVQASLYSLMSLIILATLVGNLIVIISISHFKQLHTPTNWLLHSMAIVDFL
  LGCLIMPCSMVRTVERCWYFGEILCKVHTSTDIMLSSASIFHLAFISIDRYCAVCDPLRYKAKINISTIL
  VMILVSWSLPAVYAFGMIFLELNLKGVEELYRSQVSDLGGCSPFFSKVSGVLAFMTSFYIPGSVMLFVYY
  RIYFIAKGQARSINRTNVQVGLEGKSQAPQSKETKAAKTLGIMVGVFLVCWCPFFLCTVLDPFLGYVIPP
  SLNDALYWFGYLNSALNPMVYAFFYPWFRRALKMVLLGKIFQKDSSRSKLFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053205
• ORF Size: 996 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053205.1, NP_444435.1
• RefSeq Size: 999 bp
• RefSeq ORF: 999 bp
• Locus ID: 111174
• UniProt ID: Q923Y8
• Cytogenetics: 10 A4
• MW: 38.1 kDa
• Gene Summary: Receptor for trace amines, including beta-phenylethylamine (b-PEA), p-tyramine (p-TYR), octopamine and tryptamine, with highest affinity for b-PEA and p-TYR. Unresponsive to classical biogenic amines, such as epinephrine and histamine and only partially activated by dopamine and serotonin. Trace amines are biogenic amines present in very low levels in mammalian tissues. Although some trace amines have clearly defined roles as neurotransmitters in invertebrates, the extent to which they function as true neurotransmitters in vertebrates has remained speculative. Trace amines are likely to be involved in a variety of physiological functions that have yet to be fully understood. The signal transduced by this receptor is mediated by the G(s)-class of G-proteins which activate adenylate cyclase.[UniProtKB/Swiss-Prot Function]