Lsr (NM_001164185) Mouse Tagged ORF Clone

CAT#: MR225038

Lsr (Myc-DDK-tagged) - Mouse lipolysis stimulated lipoprotein receptor (Lsr), transcript variant 3

Product Images

Circular map for MR225038

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lsr
• Synonyms: ILDR3; Lisch7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225038 representing NM_001164185
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGGCGGCCAGCGCGTGTGCTGGGGCGCCTGGCTCCCACCCGGCCACCACGATCTTCGTGTGTC
  TTTTTCTCATCATTTACTGCCCAGACCGTGCCAGTGCCATCCAGGTGACCGTGCCTGACCCCTACCACGT
  AGTGATCCTGTTCCAGCCAGTGACACTACACTGCACCTACCAGATGAGCAATACCCTCACAGCCCCTATC
  GTGATCTGGAAGTATAAGTCGTTCTGTCGGGACCGTGTTGCCGACGCCTTCTCCCCTGCCAGCGTGGACA
  ACCAGCTCAACGCCCAGCTGGCGGCTGGCAACCCCGGCTACAACCCCTATGTGGAGTGCCAGGACAGCGT
  ACGCACTGTCAGGGTGGTGGCCACCAAACAGGGCAATGCTGTGACCCTGGGAGACTACTACCAGGGCAGG
  AGAATCACCATCACAGGAAATGCTGACCTGACCTTCGAGCAGACGGCCTGGGGAGACAGTGGAGTGTATT
  ACTGCTCCGTGGTCTCAGCCCAAGATCTGGATGGGAACAACGAGGCGTACGCAGAGCTCATTGTCCTTGT
  TTATGCTGCTGGCAAAGCAGCCACCTCAGGTGTGCCAAGCATCTATGCCCCCAGCATCTATACCCACCTC
  TCTCCTGCCAAGACTCCGCCACCTCCGCCTGCCATGATTCCCATGCGTCCTCCCTATGGGTACCCTGGAG
  ACTTTGACAGGACCAGCTCAGTTGGTGGCCACAGCTCCCAGGTGCCCCTGCTGCGTGAAGTGGATGGGAG
  CGTATCTTCAGAAGTACGAAGTGGCTACAGGATCCAGGCTAACCAGCAAGATGACTCCATGAGGGTCCTA
  TACTATATGGAGAAGGAGCTAGCCAACTTCGATCCTTCCCGGCCTGGCCCTCCCAATGGCCGAGTGGAAC
  GGGCCATGAGTGAAGTAACCTCCCTCCATGAAGATGACTGGCGATCTCGGCCTTCCAGGGCTCCTGCCCT
  CACACCCATCAGGGATGAGGAGTGGAATCGCCACTCCCCTCGGAGTCCCAGAACATGGGAGCAGGAACCC
  CTTCAAGAACAGCCAAGGGGTGGTTGGGGGTCTGGGCGGCCTCGGGCCCGCTCTGTGGATGCTCTAGATG
  ACATCAACCGGCCTGGCTCCACTGAATCAGGAAGGTCTTCTCCCCCAAGTAGTGGACGGAGAGGGCGGGC
  CTATGCACCTCCGAGAAGTCGCAGCCGGGATGACCTCTATGACCCCGACGATCCTAGAGACTTGCCACAT
  TCCCGAGATCCCCACTATTATGATGATTTGAGGTCTAGGGATCCACGTGCTGACCCCAGATCCCGTCAGC
  GATCCCACGATCCTCGGGATGCTGGCTTCAGGTCACGGGACCCTCAGTATGATGGGCGACTCTTAGAAGA
  GGCTTTAAAGAAAAAAGGGGCTGGGGAGAGAAGACGCGTTTACAGGGAGGAAGAAGAAGAAGAAGAGGAG
  GGCCACTATCCCCCAGCACCTCCGCCTTACTCTGAGACTGACTCGCAGGCCTCGAGGGAGCGGAGGATGA
  AAAAGAATTTGGCCCTGAGTCGGGAAAGTTTAGTCGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225038 representing NM_001164185
  Red=Cloning site Green=Tags(s)
  
  MAPAASACAGAPGSHPATTIFVCLFLIIYCPDRASAIQVTVPDPYHVVILFQPVTLHCTYQMSNTLTAPI
  VIWKYKSFCRDRVADAFSPASVDNQLNAQLAAGNPGYNPYVECQDSVRTVRVVATKQGNAVTLGDYYQGR
  RITITGNADLTFEQTAWGDSGVYYCSVVSAQDLDGNNEAYAELIVLVYAAGKAATSGVPSIYAPSIYTHL
  SPAKTPPPPPAMIPMRPPYGYPGDFDRTSSVGGHSSQVPLLREVDGSVSSEVRSGYRIQANQQDDSMRVL
  YYMEKELANFDPSRPGPPNGRVERAMSEVTSLHEDDWRSRPSRAPALTPIRDEEWNRHSPRSPRTWEQEP
  LQEQPRGGWGSGRPRARSVDALDDINRPGSTESGRSSPPSSGRRGRAYAPPRSRSRDDLYDPDDPRDLPH
  SRDPHYYDDLRSRDPRADPRSRQRSHDPRDAGFRSRDPQYDGRLLEEALKKKGAGERRRVYREEEEEEEE
  GHYPPAPPPYSETDSQASRERRMKKNLALSRESLVV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164185
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164185.1, NP_001157657.1
• RefSeq Size: 1973 bp
• RefSeq ORF: 1581 bp
• Locus ID: 54135
• UniProt ID: Q99KG5
• Cytogenetics: 7 B1
• MW: 59.1 kDa
• Gene Summary: Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells (By similarity).[UniProtKB/Swiss-Prot Function]