Nwd1 (NM_176940) Mouse Tagged ORF Clone

CAT#: MR224981

Nwd1 (Myc-DDK-tagged) - Mouse NACHT and WD repeat domain containing 1 (Nwd1)

Product Images

Circular map for MR224981

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nwd1
• Synonyms: A230063L24Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224981 representing NM_176940
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACAGAGCGAGAAGCCCTGCAGTGCACAGCCTACCCTGAGGTTCAGAGCTTCTGCCAAAGGCACG
  GCTTGGCGTTTGAGGTGGTTGATCTGAGGTGGGGTATTCCGAATACGCAGGCCACTGACTACCTGACCAC
  GGAACTCTGCTTAGAAGAACTGGAGCGCTGTCAGAAAACATCCATAGGACCAGCTTTTGTGGCCCTCCTG
  GGTGACCAGTACGGCCCCTGCCCGGTTCCCCGGAGGATTGAGGAAAAGGAGTGGGAGGCACTAAGAGCTC
  AGCTGACCTCCAGGCCTCGAGATCTGGAGCTGGTGACCAGACATTTCCAGAGAGACGACAACACCATTCC
  TCCAACCTACGTCTTGCAGCCTTCAGGCTCACTGGTGGTCCCAGGGCCTGAGGAGGCCACCTTAACCTCT
  GTCCTGAGAGGTGGAGCCCAGGAGGCCTGGAGGCTAGGCCTCATCAGCCAGGAGCAATGGATGTGCTATC
  ACCGATCAGTTATTGAGTGGGAGATCGAGCTTGGCCTACTGAGCTCAGCAAGAGGGGACCAGGGAGCTAC
  CGTCTTCCTGAGAGATGTACAGGACCTCAATAAACACATCTTGGATGATTGCTCTCTGAAGATGGTGGAT
  CGGCTGGTAGATGGCTGCCTGGATACCAATGCCCAGAGTCTTCTCAGTGGTCTTAAAGGGCGCATACTAG
  ATGCTCAGCCTGGAGCCCTAAAGTCCCACCACCTGTCCTGGAGCCGAGACCTGGTCAACCCCAAGAACAA
  GGCTCACGCTCGTTACCTAAAGCAGTTGAGCGAGCAGTTCGTGGCCAGGACCAACCATCAGGTCCTGGAG
  CAGCTTCGAGAGCTGGAGTTGGCCAGACAGGAGCTGGGTTGGCTCTACCAAGAGATCCGCCACCACCTCT
  GGCAGAGCACAGAGTCTACCAAGGTCTTCTGCGGCCACCAGGAGCTCCTGGCCCAGCTCAGGCAGCAGCT
  CAGACAGGATGAGAGCCGGACACATACTCCCTTGGTTCTCTTTGGACCACCAGGCATCGGGAAGACCTCT
  CTCATGTGCAAGCTAGCCCAGCAGGTACCAGAGCTACTTGGCCACAAGACAGTGGTTGTCCTGAGGCTGC
  TAGGGACATCAAAGCTGAGCCTGGATGCCCGCAGCCTCCTCAGGAGCCTGTCCTTCCAAGTGTGCTTGGC
  ATATGGGCTGCCACTGCCCCCAGCCCAGGTCCTGGAGGCCCACAGCAGGGTGGGCCATTTCTTCCATACT
  CTCCTCCACACCGTGTCACAGAGGAACTTTGAGTCCCTGGTGTTGCTCCTGGACTCCGTGGATGACCTGG
  ACTCCATATGCCATTCTCCGAGGGTCTCTTGGCTGCCCCTCAAGTGTCCTCCAAGGGTGCACCTCATCCT
  CTCTACATGCTCAGGCCAGCAGGTTTTACATAACCTGCAACAGACCCTCAAGGACCCCAGCACCTACTGG
  GAGGTGAAGGCCCTGTCTGGAAGTCAAGGCCAGGAGTTCATACAGCTGCTGCTGGCTGCAGAGAAGAGGA
  TGCTGAGCCCGGGTCAGAGAGATGTCCTCTGGGCCAGCCTCCCAGAGTGTGGCCACCCAGGGAGGCTAAG
  GCTGGCCTTTGAGGAGGCCCGGAAGTGGGCCTCCTTCACTGTGCCAGTGCCCTTGGCCACTACAGCAGAG
  GAAGCCACACACCAGCTCTGTATACGCCTGGAGGAGACACATGGGGCACTGCTGGTGGCTCATGTACTGG
  GCTATATTGTGTCTTCCAGGTACGGTCTCTCAGAAGCAGAGCTGAAGGATGTGTTGTCTTTGGATGATGA
  AGTCTTGCAGGCTGTGTACCGGGACTGGACGCCACCTAGCAAAGAGCTGCTGCGCTTCCCGCCCCTGCTG
  TGGGTGCGGCTCCGCCGGGACCTGGGACACTGCCTAGTCAGGAGGCCCGTGGATGGCTGCATGCTACTGG
  CCATTGCACACAGACAGCTGTCCCAGGTAATCCAAGTTCGATATCTGTCTGGGCCTGAGAGAGCCAAGAG
  GCATGGCGTGCTAGCTGAATTCTTCTCTGGAGCCTGGAGCCAGGGGATCAAGAAACTTATCACCCTGCCG
  CTTGTAGGGAAGCCCCTGAACCTGGACCGCAAGGTGGCCCCACAGCCACTGTGGTTCTCAAGCACAGTGG
  CCAACCTGAGGAAGCTGACCGAACTGCCCTTCCATCTGCTGCATGCAGGCCGCTTGGAGGAGTTGAAGCA
  GGAGGTTCTGGGGAACATGAGTTGGATCTCATGTCGGGGCATCTCTGGAGGCATTGAGGTCCTGCTGGAT
  GATTTTGACATGTGTGCCCCTCACATGAACTCTCCTGAGGTTGATCTGGTTAGAGAAGCCATCCAGCTGT
  GTGGCCCTGCTGTGGAGCTCCGGGGCCTGGAGAAGAGCGTCCTATACACCGAGCTCCTGGCCAGACTCCT
  TTTCTTTGCTGCATCACATCCAGCAATGATCGGACAACTGTGCCAACAAGCCCAGAGCTGGTTCCGTGCC
  TGCCCCTACCCAATGCTGGTGCCCCTGGCAGGATTCCTGCAGCCACCTGGTGGACCTCTTCGAGCCACTC
  TTACTGGGTGTCACAAAGGCATCACCGCCATAGCATGGAGCCTGGAGGAGAAACTCCTGGTGGTGGGCAC
  CCAGGATGGTGCCATGGTCGTGTGGGACGTGGAGGAACAACAGGTGGTACACGTTCTAATGGGACACACA
  GCTGAAGTGAAGTGTGTGCGAGTGTTTGCCCAAGGGACTCTTGCCATCTCTGCATCAAAGGACCATACCC
  TGCGTCTGTGGAGTCTGCTTTCCGGCCAGGAGAAAGTCACCATTTTGGATGGAGGGTCACAAAATCCCAC
  AGAGCCCCAGAGTTGGGACCTTCATGTGGATGAGAGAAACAATGTTGTATACTCGACATCTGGTGCAAGG
  ATCAACATGTGGAATCTGGAAACTTCGAAGCTGGTTTTCTGTATCACAGGAGATGTGTCTGATCCCTGGG
  TCTGTGTGGCTTTGTTGGCTGCCCAGGGTTTGCTGCTTGCCCTGTCCAAGGGTGGCCAGGTCAGTCTGTG
  GAGCTCAGCCATGGGAAAACTGCAGGAGAAACACCAGTTATCCAGCATCAAAGAAGAGACACCCACCTGT
  GCCGTCTCCATCCAGTCCCGGGCAAGGCTGGTCGCTGGCTTCAGCAGTGGCTCCATTGCCCTGGTCTCCG
  CTGGAGAAGACAGGCTGCTGGAGAAGCTCCCAGAAGCTGTGGGGTTCCTGGTGGTTTCTGAAGACGATTC
  CCTTCTTGTGGCAGGCTTTGGAAGATTTGTGCGGATTTTCTTGGCTGACTCGCAAGGCTTCCACCGGTTC
  ATGGCTTCGGATCTGGAACACGAGGACATGGTGGAGACAGCAGTCTTGGGTCCTGAGAACAATCTGATCA
  TCACAGGGTCCCGGGATGCACTTATCCAGGTCTGGGATCTCAAGTCCACTAAGAAGCTGCAGTCTCCGAC
  TCCATTTCTGGACCGCACCGGCCTCGCTGCAGTGTCCCACCATGGCAGCTTTGTCTACTTCCCTAAGGTT
  GGGGACAAAAACAAAGTCACAATTTGGGACTTGGCAGAAGGTGAGGAGCAAGATTGCCTGGACACGTCCA
  ATGAGGTCCGCTGTCTGGAGGTGGCCGAGCAGGCCAAGCTCCTCTTCACAGGCCTGGTTTCTGGGATCGT
  GCTTGTGTTCCCCCTGAATTCCAGGCAAGATGTACTGTGCATCCCCCCACCTGAGGCACGCAAAGCTGTC
  AACTGCATGTCCCTGAGCAAGAGTGAGAATCGTCTGGCCATTGCCTATGACAACATTGTCCTGGTGCTTG
  ACATCAGCCCTGGGGACCCATGTCCGGCCATTGAAGGCCCCACCTACACCTTTTACACCCAGCTGCCTGA
  GACCATAGTGAGTGTGGCTGTCCTGGCTGACTATCGTGTGGTCTACGGCATGAGCGATGGCAGCCTCTTC
  CTGTACGACTGTGCATGCTCTAAAGTGTTCCCTCTGGAGGCCCATGGGAGCAGGGTGAGCTGTGTAGAGG
  TCAGCCACAGTGAGCAGCTAGCAGTGAGCGGGGCTGAAGATGCACTGCTATGCCTCTGGGACCTGCAGGC
  TTGCAGGGGGATGTTCGAGATGAGCTATGAGAACTCCTGCTGCAGAGGGGTGCGGTGTGCCTGCTTCTCC
  AGGGATGACAAGCATGTATTTGCAGGCATGGAGGACCGCTCTGTCACTGCCTGGAGCACGGTGGATGGCA
  CTCTTTTGGCTGTCCAGTTTGTTCATGCTGTGATCAACCGAATCATCCCTACCTCCAATGGCTTCATGGC
  CCCCACAAGCCACGGCTATCTCATCCGTGAGCGCTTCCAGTGCCCGTCAGTCAGAGCCTCCCAGCAAGAC
  CCTCTCAAGAACTTCAAGAAGGCAGTGTGGCTGGTAAAGACCAGGCAGAGGGAAGAGCTGGCTGCAGCAG
  AAGCCTCCCAGGATGCAGAACCAGTGGCCGTAGAGGGAAAGGAATCAAAGTCAAATAAACGCTCACAGGT
  CTGCCTCATACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224981 representing NM_176940
  Red=Cloning site Green=Tags(s)
  
  MDTEREALQCTAYPEVQSFCQRHGLAFEVVDLRWGIPNTQATDYLTTELCLEELERCQKTSIGPAFVALL
  GDQYGPCPVPRRIEEKEWEALRAQLTSRPRDLELVTRHFQRDDNTIPPTYVLQPSGSLVVPGPEEATLTS
  VLRGGAQEAWRLGLISQEQWMCYHRSVIEWEIELGLLSSARGDQGATVFLRDVQDLNKHILDDCSLKMVD
  RLVDGCLDTNAQSLLSGLKGRILDAQPGALKSHHLSWSRDLVNPKNKAHARYLKQLSEQFVARTNHQVLE
  QLRELELARQELGWLYQEIRHHLWQSTESTKVFCGHQELLAQLRQQLRQDESRTHTPLVLFGPPGIGKTS
  LMCKLAQQVPELLGHKTVVVLRLLGTSKLSLDARSLLRSLSFQVCLAYGLPLPPAQVLEAHSRVGHFFHT
  LLHTVSQRNFESLVLLLDSVDDLDSICHSPRVSWLPLKCPPRVHLILSTCSGQQVLHNLQQTLKDPSTYW
  EVKALSGSQGQEFIQLLLAAEKRMLSPGQRDVLWASLPECGHPGRLRLAFEEARKWASFTVPVPLATTAE
  EATHQLCIRLEETHGALLVAHVLGYIVSSRYGLSEAELKDVLSLDDEVLQAVYRDWTPPSKELLRFPPLL
  WVRLRRDLGHCLVRRPVDGCMLLAIAHRQLSQVIQVRYLSGPERAKRHGVLAEFFSGAWSQGIKKLITLP
  LVGKPLNLDRKVAPQPLWFSSTVANLRKLTELPFHLLHAGRLEELKQEVLGNMSWISCRGISGGIEVLLD
  DFDMCAPHMNSPEVDLVREAIQLCGPAVELRGLEKSVLYTELLARLLFFAASHPAMIGQLCQQAQSWFRA
  CPYPMLVPLAGFLQPPGGPLRATLTGCHKGITAIAWSLEEKLLVVGTQDGAMVVWDVEEQQVVHVLMGHT
  AEVKCVRVFAQGTLAISASKDHTLRLWSLLSGQEKVTILDGGSQNPTEPQSWDLHVDERNNVVYSTSGAR
  INMWNLETSKLVFCITGDVSDPWVCVALLAAQGLLLALSKGGQVSLWSSAMGKLQEKHQLSSIKEETPTC
  AVSIQSRARLVAGFSSGSIALVSAGEDRLLEKLPEAVGFLVVSEDDSLLVAGFGRFVRIFLADSQGFHRF
  MASDLEHEDMVETAVLGPENNLIITGSRDALIQVWDLKSTKKLQSPTPFLDRTGLAAVSHHGSFVYFPKV
  GDKNKVTIWDLAEGEEQDCLDTSNEVRCLEVAEQAKLLFTGLVSGIVLVFPLNSRQDVLCIPPPEARKAV
  NCMSLSKSENRLAIAYDNIVLVLDISPGDPCPAIEGPTYTFYTQLPETIVSVAVLADYRVVYGMSDGSLF
  LYDCACSKVFPLEAHGSRVSCVEVSHSEQLAVSGAEDALLCLWDLQACRGMFEMSYENSCCRGVRCACFS
  RDDKHVFAGMEDRSVTAWSTVDGTLLAVQFVHAVINRIIPTSNGFMAPTSHGYLIRERFQCPSVRASQQD
  PLKNFKKAVWLVKTRQREELAAAEASQDAEPVAVEGKESKSNKRSQVCLIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176940
• ORF Size: 4563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176940.5, NP_795914.3
• RefSeq Size: 7732 bp
• RefSeq ORF: 4566 bp
• Locus ID: 319555
• Cytogenetics: 8 B3.3
• MW: 169 kDa
• Gene Summary: May play a role in the control of androgen receptor (AR) protein steady-state levels.[UniProtKB/Swiss-Prot Function]