Ak5 (NM_001081277) Mouse Tagged ORF Clone

CAT#: MR224918

Ak5 (Myc-DDK-tagged) - Mouse adenylate kinase 5 (Ak5)

Product Images

Circular map for MR224918

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ak5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224918 representing NM_001081277
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACCAACGACGCCAAGGAGTACCTGGCCCGGAGGGACATTCCTCAGCTTTTCGAGAGCCTGCTCA
  ATGGCTTGATGTGTTCCAAGCCAGAAGACCCCATAGAGTACTTGGAAACTTGTTTACAGAAAGTCAAGGA
  GCTAGGAGGCTGTGACAAGGTGAAGTGGGACACATTTGTCAGCCAGGAAAAGAAGACGTTACCGCCCCTA
  AATGGAGGACAATCACGGAGATCCTTCCTCCGAAATGTCATGCCTGAAAATTCAAACTTTCCATATCGGC
  GGTATGACCGGCTCCCTCCAATCCACCAGTTTTCCATAGAGAGTGACACGGACCTGTCGGAGACGGCGGA
  GCTGATAGAAGAATATGAGGTTTTTGACCCGACTAGACCTCGGCCCAAAATCATTCTTGTCATAGGTGGT
  CCAGGAAGTGGAAAGGGTACTCAGAGTTTGAAAATTGCAGAGCGGTATGGATTCCAGTACATCTCAGTGG
  GAGAATTATTGAGGAAGAAAATCCACAGTGCCAGCAGCAACAGAAAATGGAGTCTGATCGCCAAGATAAT
  CACCAATGGGGAGCTGGCCCCACAGGAGACAACAATTACGGAGATAAAACAAAAATTGATGCAAATACCC
  GATGAAGAAGGCATTGTGATTGATGGATTCCCAAGGGATGTTGCCCAGGCTCTGTCTTTTGAGGACCAGA
  TCTGCACCCCTGACCTGGTGGTGTTCCTGGCTTGTGCCAACCAGAGGCTCAAGGAGAGGTTACAGAAGCG
  TGCGGAGCAGCAGGGGCGGCCAGATGACAACCTGAAAGCAACCCAAAGGAGACTAGTGAACTTCAAGCAG
  AATGCCGCCCCACTAGTGAAGTACTTCCAAGAGAAGGGACTCATCGTGACTTTTGATGCCGACCGAGATG
  AAGACGCAGTGTTCCATGACATCAGCGTGGCCGTCGACAGCAAGCTGTTTCCAAACAAAGAGGCTCCGAT
  GGATTCAAGTGACCTTGACCCTTCCATGATGTTTGATGCTGGAGAGATTATTGACACAGGATCAGATTAC
  GATAACCAGGACGATGATCAGCTAAATGTATTTGGAGAAGACACAGAGGGAGGCTTCATGGAGGATTTGA
  GGAAGTGTAAAATTATTTTCTTGATGGGTGGCCCTGGCTCTGGCAAAGGCACCCAGTGTGAGAAGCTGGC
  AGAAAAATATGGCTTCACCCATCTCTCTACTGGTGAGCTCCTGCGCCAGGAACTGACCTCAGAGTCAGAA
  AGAAGCAAACTGATCCGTGACATCATGGAGCGTGGAGATCTGGTGCCCTCGGGTGTCGTTCTGGAGCTGC
  TGAAGGAGGCCATGGTGGCCAGCCTGGGCAACACCAAGGGCTTCCTGATTGACGGCTACCCCCGGGAAGT
  GAAGCAAGGGGAAGAGTTTGGACGAAGGATCGGAGACCCACACTTGGTGATCTGCATGGACTGCTCAGCA
  GACACCATGACTAACCGCCTTCTTCAGAGAAGCCAGAGCAGCCAGCGGGGGGAGGACGGTGCCAAGAGCA
  TTGCCAAGCGCCTGGAGGCCTACCACCGAGCGTCCATCCCCGTCGTCACCTACTACGAGAGGAAAACACA
  GCTACGGAAGGTAAATGCAGAGGGAACACCAGAGCAAGTTTTTCTTCAGCTCTGCACAGCTATTGACTCT
  GTTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224918 representing NM_001081277
  Red=Cloning site Green=Tags(s)
  
  MNTNDAKEYLARRDIPQLFESLLNGLMCSKPEDPIEYLETCLQKVKELGGCDKVKWDTFVSQEKKTLPPL
  NGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDLSETAELIEEYEVFDPTRPRPKIILVIGG
  PGSGKGTQSLKIAERYGFQYISVGELLRKKIHSASSNRKWSLIAKIITNGELAPQETTITEIKQKLMQIP
  DEEGIVIDGFPRDVAQALSFEDQICTPDLVVFLACANQRLKERLQKRAEQQGRPDDNLKATQRRLVNFKQ
  NAAPLVKYFQEKGLIVTFDADRDEDAVFHDISVAVDSKLFPNKEAPMDSSDLDPSMMFDAGEIIDTGSDY
  DNQDDDQLNVFGEDTEGGFMEDLRKCKIIFLMGGPGSGKGTQCEKLAEKYGFTHLSTGELLRQELTSESE
  RSKLIRDIMERGDLVPSGVVLELLKEAMVASLGNTKGFLIDGYPREVKQGEEFGRRIGDPHLVICMDCSA
  DTMTNRLLQRSQSSQRGEDGAKSIAKRLEAYHRASIPVVTYYERKTQLRKVNAEGTPEQVFLQLCTAIDS
  VF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081277
• ORF Size: 1686 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081277.2
• RefSeq Size: 3103 bp
• RefSeq ORF: 1689 bp
• Locus ID: 229949
• UniProt ID: Q920P5
• Cytogenetics: 3 H3
• MW: 63.8 kDa
• Gene Summary: Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.[UniProtKB/Swiss-Prot Function]