Arhgap27 (NM_133715) Mouse Tagged ORF Clone

CAT#: MR224354

Arhgap27 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 27 (Arhgap27), transcript variant 2

Product Images

Circular map for MR224354

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap27
• Synonyms: 2310069I04Rik; 5730442P18Rik; Gm21552; Sh3d20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224354 representing NM_133715
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGATATGATTTCAAAACTGGTCAGAAGGCAGAGCCGGGCCCTGCGGGCACAGGTGGACGACCCCC
  CGGAACCAGTGTATGCCAATGTAGAGAGGCAACCTCGGGCCACGTCGCCTCGCTCAGCTGCAGCTCCTCC
  TCGGCTCAGCCCAGTGTGGGAGACACACACAGACACGGGCACTGGGCGCCCCTACTACTACAACCCCGAC
  ACGGGCGTGACCACCTGGGAGTCACCCTTTGAAACACCTGAAGGCACCACCAGCCCGGCCACCTCCCGGG
  CCTCTGTGGGTAGCGGGGAGAGCCTGGAGACAGAGTGGGGCCAGTACTGGGATGAGGAGAGCCGCAGGGT
  CTTCTTCTACAACCCCTTGACCGGTGAGACGGCCTGGGAGGACGAGACCGAGGAGCTGGAGGAAGATCAC
  CAGGAACAACTGGAAATGCAACCCAGCCTGAGCCCGCGAAGCCCCGGGCAGCAGAGGCCCCCAACTCCTG
  AAACAGACTACCCCGAATTACTGGCCAGTTACCCTGAAGAGGACTACTCTCCTGTGGGCTCCTTCAGTGA
  TCCGGGCCCCGCTTCTCCTTTGGTTGCACCCCCTGGCTGGTCCTGTCAAATTACCCCAGACAAACAGATG
  CTCTACACCAACCAGTTCACTCAAGAGCAGTGGGTAAGGTTGGAGGACCAGCACGGGAAGCCATATTTCT
  ACAACCCAGAGGACTCCTCTGTTCAGTGGGAGCTACCCCAGGTCCCGATCCCTGCCCCCCGAAGTGTCCG
  CAAATCCAGCCAGGATAGCGACACTCCAGCCCAGGCTAGCCCTCCAGAGGAGAAGATCAAGACTCTGGAC
  AAGGCAGGAGTCCTCCATCGTACCAAGACGGTAGACAAGGGGAAGAGACTTCGGAAGAAACACTGGAGCA
  CCTCCTGGACAGTGTTGGAGGGCGGGGTCCTGACCTTCTTCAAGGACTCAAAGACCTCAGCTGCAGGCGG
  CCTGAGGCAGCCTTCCAAGCTCTCCACCCCTGAATACACAGTCGAACTGAAGGGGGCCTCTCTCTCTTGG
  GCCCCCAAAGACAAATCCAGCAAGAAGAATGTGTTAGAGCTTCGAAGCCGAGATGGCTCGGAGTACCTGA
  TCCAGCACGACTCCGAAGCCATCATCAGCACCTGGCACAAAGCCATTGCCGAAGGCATCTCAGAGCTGTC
  TGCAGACCTGCTCCAAGGGGAGGAAGGTGAGCCCAGCAGTGCTGATTTCGGGTCCAGTGAGCGCCTCGGA
  AGCTGGCGGGAGGAGGATGTACGGCAGAACGCAGCCTCACCCTCGCTCAGCCCCGGAGGCCTGGAAAGCG
  ACTTGAGCAGAGTCCGGCACAAGCTCCGTAAATTCCTACAGAGACGACCCACTCTGCAGTCTTTGCGGGA
  CAAGGGCTACATCAAAGACCAGGTGTTTGGATGCGCGCTGGCTCAACTGTGTGAGCGCGAGAGGAGCCCT
  GTGCCACGCTTCGTGCAGCAATGCATCCGCACCGTCGAGGCCCGGGGGCTGGATATTGATGGGCTGTACC
  GCATCAGTGGGAACCTGGCTACCATCCAGAAGCTGCGCTATAAGGTGGATCACGATGAACGCTTGGACTT
  GGACGACGGGCGCTGGGAAGACGTCCACGTGATCACGGGTGCCCTGAAGCTCTTCTTCCGAGAGCTGCCG
  GAACCTCTCTTCCCCTTCTCGCACTTCCACCAGTTCATAGCAGCCATTAAGCTGCAGGACCCAGCCCAGC
  GCAGCCGCTGCGTGCGTGACCTGGTGCGCACGCTGCCAGCTCCCAACCAGGACACGCTCCGACTGCTCAT
  CCAGCATCTCTGCCGGGTCATCGAGCATGGCGAGCAGAACCGTATGACTGTGCAGAATGTGGCCATAGTG
  TTCGGACCCACGCTGCTGCGGCCAGAGATGGAGGAGGCCAGCATGCCCATGACCATGGTGTTCCAGAACC
  AGGTGGTGGAGCTCATCCTACATCAGTGCGCGGACATCTTCCCACCGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224354 representing NM_133715
  Red=Cloning site Green=Tags(s)
  
  MVDMISKLVRRQSRALRAQVDDPPEPVYANVERQPRATSPRSAAAPPRLSPVWETHTDTGTGRPYYYNPD
  TGVTTWESPFETPEGTTSPATSRASVGSGESLETEWGQYWDEESRRVFFYNPLTGETAWEDETEELEEDH
  QEQLEMQPSLSPRSPGQQRPPTPETDYPELLASYPEEDYSPVGSFSDPGPASPLVAPPGWSCQITPDKQM
  LYTNQFTQEQWVRLEDQHGKPYFYNPEDSSVQWELPQVPIPAPRSVRKSSQDSDTPAQASPPEEKIKTLD
  KAGVLHRTKTVDKGKRLRKKHWSTSWTVLEGGVLTFFKDSKTSAAGGLRQPSKLSTPEYTVELKGASLSW
  APKDKSSKKNVLELRSRDGSEYLIQHDSEAIISTWHKAIAEGISELSADLLQGEEGEPSSADFGSSERLG
  SWREEDVRQNAASPSLSPGGLESDLSRVRHKLRKFLQRRPTLQSLRDKGYIKDQVFGCALAQLCERERSP
  VPRFVQQCIRTVEARGLDIDGLYRISGNLATIQKLRYKVDHDERLDLDDGRWEDVHVITGALKLFFRELP
  EPLFPFSHFHQFIAAIKLQDPAQRSRCVRDLVRTLPAPNQDTLRLLIQHLCRVIEHGEQNRMTVQNVAIV
  FGPTLLRPEMEEASMPMTMVFQNQVVELILHQCADIFPPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133715
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133715.5, NP_598476.2
• RefSeq Size: 3528 bp
• RefSeq ORF: 2013 bp
• Locus ID: 544817
• UniProt ID: A2AB59
• Cytogenetics: 11 E1
• MW: 75.8 kDa
• Gene Summary: Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).[UniProtKB/Swiss-Prot Function]