Arhgap27 (NM_133715) Mouse Untagged Clone

CAT#: MC220396

Arhgap27 (untagged) - Mouse Rho GTPase activating protein 27 (Arhgap27), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arhgap27
• Synonyms: 2310069I04Rik; 5730442P18Rik; Gm21552; Sh3d20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220396 representing NM_133715
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGATATGATTTCAAAACTGGTCAGAAGGCAGAGCCGGGCCCTGCGGGCACAGGTGGACGACCCCC
  CGGAACCAGTGTATGCCAATGTAGAGAGGCAACCTCGGGCCACGTCGCCTCGCTCAGCTGCAGCTCCTCC
  TCGGCTCAGCCCAGTGTGGGAGACACACACAGACACGGGCACTGGGCGCCCCTACTACTACAACCCCGAC
  ACGGGCGTGACCACCTGGGAGTCACCCTTTGAAACACCTGAAGGCACCACCAGCCCGGCCACCTCCCGGG
  CCTCTGTGGGTAGCGGGGAGAGCCTGGAGACAGAGTGGGGCCAGTACTGGGATGAGGAGAGCCGCAGGGT
  CTTCTTCTACAACCCCTTGACCGGTGAGACGGCCTGGGAGGACGAGACCGAGGAGCTGGAGGAAGATCAC
  CAGGAACAACTGGAAATGCAACCCAGCCTGAGCCCGCGAAGCCCCGGGCAGCAGAGGCCCCCAACTCCTG
  AAACAGACTACCCCGAATTACTGGCCAGTTACCCTGAAGAGGACTACTCTCCTGTGGGCTCCTTCAGTGA
  TCCGGGCCCCGCTTCTCCTTTGGTTGCACCCCCTGGCTGGTCCTGTCAAATTACCCCAGACAAACAGATG
  CTCTACACCAACCAGTTCACTCAAGAGCAGTGGGTAAGGTTGGAGGACCAGCACGGGAAGCCATATTTCT
  ACAACCCAGAGGACTCCTCTGTTCAGTGGGAGCTACCCCAGGTCCCGATCCCTGCCCCCCGAAGTGTCCG
  CAAATCCAGCCAGGATAGCGACACTCCAGCCCAGGCTAGCCCTCCAGAGGAGAAGATCAAGACTCTGGAC
  AAGGCAGGAGTCCTCCATCGTACCAAGACGGTAGACAAGGGGAAGAGACTTCGGAAGAAACACTGGAGCA
  CCTCCTGGACAGTGTTGGAGGGCGGGGTCCTGACCTTCTTCAAGGACTCAAAGACCTCAGCTGCAGGCGG
  CCTGAGGCAGCCTTCCAAGCTCTCCACCCCTGAATACACAGTCGAACTGAAGGGGGCCTCTCTCTCTTGG
  GCCCCCAAAGACAAATCCAGCAAGAAGAATGTGTTAGAGCTTCGAAGCCGAGATGGCTCGGAGTACCTGA
  TCCAGCACGACTCCGAAGCCATCATCAGCACCTGGCACAAAGCCATTGCCGAAGGCATCTCAGAGCTGTC
  TGCAGACCTGCTCCAAGGGGAGGAAGGTGAGCCCAGCAGTGCTGATTTCGGGTCCAGTGAGCGCCTCGGA
  AGCTGGCGGGAGGAGGATGTACGGCAGAACGCAGCCTCACCCTCGCTCAGCCCCGGAGGCCTGGAAAGCG
  ACTTGAGCAGAGTCCGGCACAAGCTCCGTAAATTCCTACAGAGACGACCCACTCTGCAGTCTTTGCGGGA
  CAAGGGCTACATCAAAGACCAGGTGTTTGGATGCGCGCTGGCTCAACTGTGTGAGCGCGAGAGGAGCCCT
  GTGCCACGCTTCGTGCAGCAATGCATCCGCACCGTCGAGGCCCGGGGGCTGGATATTGATGGGCTGTACC
  GCATCAGTGGGAACCTGGCTACCATCCAGAAGCTGCGCTATAAGGTGGATCACGATGAACGCTTGGACTT
  GGACGACGGGCGCTGGGAAGACGTCCACGTGATCACGGGTGCCCTGAAGCTCTTCTTCCGAGAGCTGCCG
  GAACCTCTCTTCCCCTTCTCGCACTTCCACCAGTTCATAGCAGCCATTAAGCTGCAGGACCCAGCCCAGC
  GCAGCCGCTGCGTGCGTGACCTGGTGCGCACGCTGCCAGCTCCCAACCAGGACACGCTCCGACTGCTCAT
  CCAGCATCTCTGCCGGGTCATCGAGCATGGCGAGCAGAACCGTATGACTGTGCAGAATGTGGCCATAGTG
  TTCGGACCCACGCTGCTGCGGCCAGAGATGGAGGAGGCCAGCATGCCCATGACCATGGTGTTCCAGAACC
  AGGTGGTGGAGCTCATCCTACATCAGTGCGCGGACATCTTCCCACCGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133715
• Insert Size: 2013 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133715.5, NP_598476.2
• RefSeq Size: 3528 bp
• RefSeq ORF: 2013 bp
• Locus ID: 544817
• UniProt ID: A2AB59
• Cytogenetics: 11 E1
• Gene Summary: Rho GTPase-activating protein which may be involved in clathrin-mediated endocytosis. GTPase activators for the Rho-type GTPases act by converting them to an inactive GDP-bound state. Has activity toward CDC42 and RAC1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate internal promoter, compared to variant 1. The encoded isoform (2) has a distinct and shorter N-terminus, compared to isoform 1.