Ddr1 (NM_172962) Mouse Tagged ORF Clone

CAT#: MR224276

Ddr1 (Myc-DDK-tagged) - Mouse discoidin domain receptor family, member 1 (Ddr1), transcript variant 2

Product Images

Circular map for MR224276

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddr1
• Synonyms: 6030432F18; AI323681; Cak; CD167a; Nep; PTK3A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224276 representing NM_172962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAGGGACCCTCTCATCTCTACTGCTGCTGCTACTCTTGGTGACAATTGGAGATGCTGACATGA
  AGGGACATTTTGACCCTGCCAAGTGCCGCTATGCCCTGGGCATGCAGGACCGCACCATTCCTGACAGCGA
  TATCTCTGTGTCCAGCTCCTGGTCGGACTCTACCGCTGCCCGCCACAGCAGGCTGGAAAGCAGTGATGGA
  GATGGGGCTTGGTGCCCTGCAGGGCCTGTGTTCCCCAAAGAAGAGGAGTACTTGCAGGTGGACCTTCGTA
  GGCTACACCTGGTGGCTCTGGTGGGCACCCAGGGCCGCCATGCTGGGGGTCTGGGCAAAGAGTTCTCCCG
  AAGCTATCGGTTGCGTTACTCCCGAGATGGCCGCCGCTGGATGGACTGGAAGGACCGCTGGGGACAGGAG
  GTGATTTCGGGTAACGAGGATCCCGGGGGAGTAGTGCTGAAGGACCTTGGGCCCCCCATGGTGGCCCGGC
  TGGTCCGCTTCTACCCCAGGGCTGACCGGGTCATGAGTGTCTGTCTTCGGGTGGAGCTCTATGGCTGCCT
  CTGGCGGGATGGACTCCTGTCATATACAGCCCCCGTGGGGCAGACCATGCAGTTATCTGAGGTGATGGTA
  CATCTCAATGATTCCACTTACGATGGATATACTGCTGGAGGGCTGCAGTATGGCGGTCTGGGCCAGCTGG
  CAGATGGCGTGGTGGGCCTGGATGATTTCAGGCAGAGCCAGGAGCTGCGGGTCTGGCCAGGCTATGACTA
  TGTGGGATGGAGCAATCAGAGCTTCCCCACGGGCTACGTGGAGATGGAGTTTGAGTTTGATCGGTTGAGG
  ACCTTCCAGACCATGCAGGTCCACTGTAACAACATGCACACTCTGGGAGCCCGCCTACCAGGCGGGGTGG
  AATGCCGGTTTAAAAGGGGTCCCGCCATGGCCTGGGAAGGAGAGCCTGTCCGCCATGCTCTGGGAGGCAG
  CCTTGGAGACCCCAGAGCCCGGGCCATCTCAGTGCCCCTGGGTGGCCACGTGGGCCGCTTTCTGCAGTGC
  AGATTCCTCTTTGCAGGTCCTTGGTTACTCTTCAGTGAGATCTCTTTCATCTCAGATGTGGTGAACGACT
  CCTCTGACACCTTCCCACCAGCCCCCTGGTGGCCACCTGGCCCGCCTCCCACCAACTTCAGCAGCTTGGA
  GCTGGAGCCCCGGGGTCAACAGCCAGTGGCCAAGGCGGAGGGGAGCCCAACTGCCATCCTCATTGGCTGC
  CTGGTGGCCATCATCCTGCTGCTGCTTCTCATCATCGCGCTGATGCTCTGGAGGCTGCACTGGCGCCGGC
  TGCTCAGCAAGGCTGAGCGCCGCGTGTTGGAGGAGGAGCTGACGGTTCACCTTTCTGTCCCTGGGGACAC
  CATCCTCATCAACAACCGCCCAGGACCCCGAGAGCCACCCCCTTACCAGGAGCCCCGGCCTCGGGGGACT
  CCACCCCATTCTGCACCCTGCGTCCCCAACGGCTCTGCCTGCAGTGGGGACTATATGGAGCCCGAGAAGC
  CGGGTGCCCCGCTTCTACCCCCACCTCCCCAGAACAGCGTCCCCCATTATGCCGAAGCTGACATTGTCAC
  CCTGCAGGGCGTCACTGGGGGCAACACCTACGCTGTGCCTGCACTGCCCCCAGGGGCGGTTGGGGATGGG
  CCCCCCAGAGTGGATTTCCCTCGGTCACGGCTCCGCTTCAAGGAGAAGCTTGGCGAGGGCCAATTTGGGG
  AGGTACACCTGTGTGAAGTAGAGGACCCGCAAGATCTGGTCAGTAGTGACTTCCCTATCAGTGTGCACAA
  GGGACACCCCTTGCTGGTAGCAGTGAAGATCCTCCGGCCAGATGCCACCAAAAATGCCAGGAATGATTTC
  CTGAAGGAGGTAAAGATCATGTCACGGCTGAAGGACCCAAACATCATCCGGCTCCTGGGTGTGTGTGTGC
  AGGATGACCCCCTCTGCATGATCACAGACTACATGGAGAACGGCGATCTGAACCAGTTCCTCAGTGCCCG
  CCAGCTGGAGAACAAGGCCACTCAGGGGCTCTCTGGGGACACAGAGTCTGACCAGGGGCCCACAATCAGC
  TACCCTATGCTGTTACACGTGGGGGCCCAGATCGCCTCTGGCATGCGTTATCTCGCCACGCTGAACTTTG
  TGCATCGGGACCTGGCCACCCGGAACTGCTTGGTTGGGGAAAATTTCACCATCAAAATCGCCGACTTTGG
  CATGAGCCGGAATCTCTACGCTGGGGATTATTACCGTGTCCAGGGCCGGGCGGTGCTGCCCATCAGGTGG
  ATGGCTTGGGAGTGCATTCTCATGGGGAAGTTCACAACAGCCAGTGACGTTTGGGCCTTCGGAGTGACCC
  TGTGGGAGGTGCTGATGCTCTGCAGGTCCCAGCCCTTTGGGCAGCTTACAGATGAGCAGGTTATCGAGAA
  TGCCGGCGAGTTCTTCAGGGACCAGGGCCGGCAGGTCTACTTGTCCAGGCCACCCGCCTGCCCACAGACC
  CTGTATGAGCTGATGCTCCGGTGTTGGAGCCGGGAGCCCGAGCAGCGGCCGCCCTTCGCCCAGCTTCATC
  GGTTCCTGGCGGATGATGCGCTCAACACGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224276 representing NM_172962
  Red=Cloning site Green=Tags(s)
  
  MGTGTLSSLLLLLLLVTIGDADMKGHFDPAKCRYALGMQDRTIPDSDISVSSSWSDSTAARHSRLESSDG
  DGAWCPAGPVFPKEEEYLQVDLRRLHLVALVGTQGRHAGGLGKEFSRSYRLRYSRDGRRWMDWKDRWGQE
  VISGNEDPGGVVLKDLGPPMVARLVRFYPRADRVMSVCLRVELYGCLWRDGLLSYTAPVGQTMQLSEVMV
  HLNDSTYDGYTAGGLQYGGLGQLADGVVGLDDFRQSQELRVWPGYDYVGWSNQSFPTGYVEMEFEFDRLR
  TFQTMQVHCNNMHTLGARLPGGVECRFKRGPAMAWEGEPVRHALGGSLGDPRARAISVPLGGHVGRFLQC
  RFLFAGPWLLFSEISFISDVVNDSSDTFPPAPWWPPGPPPTNFSSLELEPRGQQPVAKAEGSPTAILIGC
  LVAIILLLLLIIALMLWRLHWRRLLSKAERRVLEEELTVHLSVPGDTILINNRPGPREPPPYQEPRPRGT
  PPHSAPCVPNGSACSGDYMEPEKPGAPLLPPPPQNSVPHYAEADIVTLQGVTGGNTYAVPALPPGAVGDG
  PPRVDFPRSRLRFKEKLGEGQFGEVHLCEVEDPQDLVSSDFPISVHKGHPLLVAVKILRPDATKNARNDF
  LKEVKIMSRLKDPNIIRLLGVCVQDDPLCMITDYMENGDLNQFLSARQLENKATQGLSGDTESDQGPTIS
  YPMLLHVGAQIASGMRYLATLNFVHRDLATRNCLVGENFTIKIADFGMSRNLYAGDYYRVQGRAVLPIRW
  MAWECILMGKFTTASDVWAFGVTLWEVLMLCRSQPFGQLTDEQVIENAGEFFRDQGRQVYLSRPPACPQT
  LYELMLRCWSREPEQRPPFAQLHRFLADDALNTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172962
• ORF Size: 2622 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172962.1, NP_766550.1
• RefSeq Size: 3703 bp
• RefSeq ORF: 2625 bp
• Locus ID: 12305
• UniProt ID: Q03146
• Cytogenetics: 17 18.7 cM
• MW: 97.7 kDa
• Gene Summary: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing, but also tumor cell invasion. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Phosphorylates PTPN11 (By similarity). Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing.[UniProtKB/Swiss-Prot Function]