Tomm40 (NM_016871) Mouse Tagged ORF Clone

CAT#: MR224111

Tomm40 (Myc-DDK-tagged) - Mouse translocase of outer mitochondrial membrane 40 homolog (yeast) (Tomm40), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Circular map for MR224111

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tomm40
• Synonyms: AW539759; Mom35; Tom40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224111 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACGTGTTGGCTGCCAGCTCTCCGCCCGCAGGGCCACCGCCGCCTCCTACGCCGTCCCTCGTGG
  GGCTCCCGCCGCCGCCTCCTTCGCCGCCAGGCTTCACTCTGCCGCCGCTCGGCGGCGGCCTGGGCACTGG
  GTCAAGCACTGGCCGTGGTTCGGAACGGACTCCCGGGGCTGCGGCCAGCGGCGCTGCGGCGGCCTCGGAA
  GATGGGAGCTGCGGATGCCTGCCCAACCCGGGGACGTTCGAGGAGTGCCACCGGAAGTGCAAGGAGCTGT
  TTCCAGTTCAGATGGAAGGTGTCAAACTTACAGTCAACAAAGGGTTGAGCAACCGTTTCCAGGTGACCCA
  CACAGTAGCCCTCGGCACAATTGGGGAGTCCAACTACCACTTTGGGGTCACATACGTGGGGACGAAGCAG
  CTGAGTCCCACAGAGGCGTTCCCCGTGCTGGTGGGTGACATGGACAATAGTGGCAGCCTCAATGCACAGG
  TCATCCACCAGCTGAGCCCAGGCCTCAGGTCCAAAATGGCCATCCAGACCCAGCAGTCCAAGTTCGTGAA
  CTGGCAGGTGGATGGCGAGTACCGTGGTTCTGACTTCACAGCTGCTGTCACCCTGGGCAACCCAGATGTC
  TTGGTGGGTTCAGGAATCCTCGTGGCCCACTACCTCCAGAGCATCACACCGTGTCTGGCCCTGGGTGGAG
  AGCTCGTCTACCACCGGCGGCCAGGAGAGGAGGGCACTGTCATGTCTCTAGCTGGGAAATACACACTGAA
  CAACTGGTTGGCTACAGTAACGCTGGGCCAGGCAGGCATGCATGCGACGTATTACCACAAAGCCAGTGAC
  CAGCTGCAGGTGGGTGTGGAGTTTGAGGCCAGCACCAGGATGCAGGACACCAGTGCCTCCTTTGGGTATC
  AGCTGGACCTGCCCAAGGCCAACTTCCTTTTTAAAGGCTCTGTGAACAGTAACTGGATCGTGGGCGCCAC
  GCTGGAGAAGAAGCTTCCGCCCTTGCCCCTGACACTGTCCCTCTGCGCCTTCCTGAACCACCGCAAGAAT
  AAGTTCCTGTGTGGCTTCGGCCTCACCATCGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224111 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGNVLAASSPPAGPPPPPTPSLVGLPPPPPSPPGFTLPPLGGGLGTGSSTGRGSERTPGAAASGAAAASE
  DGSCGCLPNPGTFEECHRKCKELFPVQMEGVKLTVNKGLSNRFQVTHTVALGTIGESNYHFGVTYVGTKQ
  LSPTEAFPVLVGDMDNSGSLNAQVIHQLSPGLRSKMAIQTQQSKFVNWQVDGEYRGSDFTAAVTLGNPDV
  LVGSGILVAHYLQSITPCLALGGELVYHRRPGEEGTVMSLAGKYTLNNWLATVTLGQAGMHATYYHKASD
  QLQVGVEFEASTRMQDTSASFGYQLDLPKANFLFKGSVNSNWIVGATLEKKLPPLPLTLSLCAFLNHRKN
  KFLCGFGLTIG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016871
• ORF Size: 1086 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016871.2, NP_058567.2
• RefSeq Size: 1571 bp
• RefSeq ORF: 1086 bp
• Locus ID: 53333
• UniProt ID: Q9QYA2
• Cytogenetics: 7 A3
• MW: 37.9 kDa
• Gene Summary: Channel-forming protein essential for import of protein precursors into mitochondria.[UniProtKB/Swiss-Prot Function]