Brd7 (NM_012047) Mouse Tagged ORF Clone

CAT#: MR224080

Brd7 (Myc-DDK-tagged) - Mouse bromodomain containing 7 (Brd7)

Product Images

Circular map for MR224080

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brd7
• Synonyms: BP75; CELTIX1; Ptpn13ip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224080 representing NM_012047
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAAGCACAAGAAGCACAAGTCGGACCGCCACTTCTACGAGGAGTACGTGGAGAAGCCCCTGA
  AGCTGGTCCTCAAAGTCGGGGGGAGCGAGGTCACCGAGCTCTCCACGGGCAGCTCCGGGCACGACTCCAG
  CCTCTTCGAAGACAGAAGCGACCATGACAAACACAAGGACAGAAAACGGAAAAAGAGGAAGAAAGGCGAG
  AAGCAGGCTCCGGGGGAAGAGAAGGGGAGAAAACGGAGAAGAGTCAAGGAGGATAAAAAGAAGCGGGATC
  GAGACCGTGCAGAGAATGAGGTGGACAGAGATCTCCAGTGTCATGTCCCTATAAGATTAGACTTACCTCC
  TGAGAAGCCTCTTACAAGCTCGTTAGCCAAACAAGAAGAAGTAGAACAGACACCCCTTCAGGAAGCTTTG
  AATCAGCTCATGAGACAATTGCAAAGAAAAGACCCAAGTGCTTTCTTTTCATTTCCTGTGACGGATTTTA
  TTGCGCCTGGCTACTCCATGATTATTAAACACCCAATGGATTTTAGTACCATGAAAGAAAAGATCAAGAA
  TAACGACTACCAGTCCATAGAAGAACTAAAGGATAACTTCAAGCTAATGTGTACTAATGCAATGATTTAC
  AATAAGCCAGAGACCATTTATTATAAAGCTGCAAAGAAGCTGTTGCACTCAGGGATGAAAATTCTCAGTC
  AGGAGAGAATTCAGAGCCTGAAGCAGAGTATAGACTTCATGTCAGACTTGCAGAAAACTCGGAAGCAGAA
  AGAACGAACAGATGCCTGTCAGAGTGGGGAGGACAGCGGCTGCTGGCAGCGCGAGAGGGAAGACTCTGGA
  GATGCTGAAACACAGGCCTTCAGAAGCCCCGCTAAGGACAATAAAAGGAAAGACAAAGATGTGCTTGAAG
  ACAAATGGAGAAGCAGCAACTCAGAAAGGGAGCATGAGCAGATTGAGCGCGTTGTCCAGGAGTCAGGAGG
  CAAGCTAACACGGCGGCTGGCAAACAGTCAGTGTGAATTTGAAAGAAGAAAACCAGATGGGACAACAACA
  CTGGGGCTTCTCCATCCTGTGGATCCCATTGTGGGAGAGCCAGGCTACTGCCCTGTGAGATTGGGGATGA
  CAACTGGAAGACTGCAGTCTGGAGTGAACACTCTGCAGGGGTTCAAAGAGGATAAAAGGAACAGAGTAAC
  CCCAGTATTATACTTGAATTATGGACCCTACAGTTCTTATGCCCCACATTATGACTCTACATTTGCCAAT
  ATTAGCAAAGATGATTCTGATTTAATCTACTCAACATATGGGGAAGACTCTGACCTTCCAAACAATTTCA
  GCATCTCTGAGTTTTTGGCCACATGCCAAGATTACCCGTATGTTATGGCAGATAGTTTACTGGATGTTCT
  AACAAAAGGAGGACATTCCAGGAGCCTGCAGGACTTGGACATGTCATCTCCTGAAGATGAAGGCCAGACC
  AGAGCATTGGACACAGCAAAAGAAGCAGAGATTACACAAATAGAGCCAACAGGGCGTTTGGAGTCCAGCA
  GTCAGGACAGGCTCACAGCACTGCAAGCTGTAACAACCTTTGGTGCTCCAGCTGAAGTCTTTGACTCCGA
  AGAGGCTGAGGTGTTCCAGAGGAAGCTTGATGAGACGACAAGATTGCTCAGGGAGCTCCAGGAGGCACAG
  AATGAGCGACTGAGCACTAGGCCTCCTCCCAATATGATCTGTCTCCTGGGTCCTTCTTACAGAGAAATGT
  ACCTTGCTGAACAAGTGACCAATAACCTCAAAGAACTCACACAGCAAGTGACTCCAGGTGATGTTGTAAG
  CATACACGGAGTGCGAAAAGCAATGGGGATTTCTGTTCCTTCCCCCATCGTGGGAAACAGCTTCGTAGAT
  TTGACAGGAGAGTGTGAAGAACCTAAGGAGACCAGCACTGCTGAGTGTGGGCCTGACGCGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224080 representing NM_012047
  Red=Cloning site Green=Tags(s)
  
  MGKKHKKHKSDRHFYEEYVEKPLKLVLKVGGSEVTELSTGSSGHDSSLFEDRSDHDKHKDRKRKKRKKGE
  KQAPGEEKGRKRRRVKEDKKKRDRDRAENEVDRDLQCHVPIRLDLPPEKPLTSSLAKQEEVEQTPLQEAL
  NQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIY
  NKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMSDLQKTRKQKERTDACQSGEDSGCWQREREDSG
  DAETQAFRSPAKDNKRKDKDVLEDKWRSSNSEREHEQIERVVQESGGKLTRRLANSQCEFERRKPDGTTT
  LGLLHPVDPIVGEPGYCPVRLGMTTGRLQSGVNTLQGFKEDKRNRVTPVLYLNYGPYSSYAPHYDSTFAN
  ISKDDSDLIYSTYGEDSDLPNNFSISEFLATCQDYPYVMADSLLDVLTKGGHSRSLQDLDMSSPEDEGQT
  RALDTAKEAEITQIEPTGRLESSSQDRLTALQAVTTFGAPAEVFDSEEAEVFQRKLDETTRLLRELQEAQ
  NERLSTRPPPNMICLLGPSYREMYLAEQVTNNLKELTQQVTPGDVVSIHGVRKAMGISVPSPIVGNSFVD
  LTGECEEPKETSTAECGPDAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012047
• ORF Size: 1953 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012047.3
• RefSeq Size: 2370 bp
• RefSeq ORF: 1956 bp
• Locus ID: 26992
• UniProt ID: O88665
• Cytogenetics: 8 C3
• MW: 74.4 kDa
• Gene Summary: Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase (By similarity). Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR.[UniProtKB/Swiss-Prot Function]