Ski (NM_011385) Mouse Tagged ORF Clone

CAT#: MR223989

Ski (Myc-DDK-tagged) - Mouse ski sarcoma viral oncogene homolog (avian) (Ski)

Product Images

Circular map for MR223989

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ski
• Synonyms: 2310012I02Rik; 2610001A11Rik; AA062172; AA589460; BC004088
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223989 representing NM_011385
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCTGCAGCGGCCGGCCGGGGCGGCTTCCAGCCGCACCCGGGGCTGCAGAAGACGCTGGAGCAGT
  TCCACCTGAGCTCCATGAGCTCGCTGGGCGGCCCGGCCGCTTTCTCGGCGCGCTGGGCGCAGGAGGCCTA
  CAAGAAGGAGAGCGCCAAGGAGGCGGGTGCGGCCACAGTGCCGGCGCCGGTGCCCACTGCCGCGGAGCCG
  CCGCCCGTGCTGCACCTGCCTGCCATCCAGCCGCCGCCGCCCGTGCTTCCCGGGCCCTTCTTCATGCCGT
  CGGACCGCTCCACCGAGCGCTGTGAGACCGTGCTGGAAGGGGAGACCATCTCTTGTTTCGTGGTGGGAGG
  TGAGAAGCGTCTGTGCCTGCCGCAGATCCTCAACTCGGTGCTGCGCGACTTCTCACTGCAGCAGATCAAC
  TCCGTGTGCGATGAGCTGCACATCTACTGCTCGCGCTGCACCGCCGACCAGCTGGAGATCCTCAAAGTCA
  TGGGCATCCTGCCCTTCTCCGCGCCCTCCTGCGGGCTCATCACTAAGACGGACGCCGAGCGCCTGTGCAA
  CGCGCTGCTCTACGGCGGCGCCTACCCGCCGCCTTGCAAGAAGGAGTTGGCGGCCAGCCTGGCGCTGGGC
  CTGGAGCTCAGCGAACGCAGCGTCCGCGTGTACCACGAGTGCTTCGGCAAGTGCAAAGGGCTGCTGGTGC
  CCGAGCTGTACAGCAGTCCCAGCGCTGCCTGCATCCAGTGCCTCGACTGCCGCCTCATGTACCCGCCGCA
  CAAGTTCGTTGTACACTCGCACAAGGCCCTGGAGAACCGGACCTGCCACTGGGGCTTCGACTCAGCCAAC
  TGGCGGGCCTACATCCTGCTGAGCCAGGACTACACGGGCAAGGAGGAGCAGGCCCGCCTTGGCCGCTGCC
  TGGACGACGTGAAGGAGAAGTTCGACTATGCCAACAAGTACAAGCGGAGGGTGCCCCGGGTCTCAGAGCC
  CCCAGCTTCCATAAGACCCAAAACAGACGACACTTCCTCACAGTCCCCTGCGTCTTCTGAGAAGGACAAG
  CAGTCCACTTGGCTGCGGACCTTGGCTGGTTCCTCCAATAAGAGCCTCGGCTGCACTCACCCTCGCCAGC
  GCCTTTCTGCTTTCCGGCCCTGGTCCCCTGCAGTGTCTGCGAGTGAGAAAGAGACGTCCCCACACCTCCC
  AGCTTTGATTCGAGACAGCTTCTACTCCTACAAGAGCTTTGAGACAGCCGTGGCCCCCAATGTGGCCCTA
  GCACCACCAACCCAGCAAAAGGTTGTGAACAGTCCACCCTGCACCACCGTGGTCTCCCGGGCACCAGAGC
  CTCTTACTACTTGTATCCAGCCACGAAAACGGAAGCTGACTCTGGACACAGCAGGAGCCCCAGACATGCT
  GACACCTGTGGCTGCTGCAGAGGAAGACAAGGATTCTGAGGCCGAGGTTGAAGTTGAAAGCAGAGAGGAG
  TTCACCTCCTCCTTGTCCTCGCTGTCCTCTCCGTCCTTTACCTCGTCCAGCTCTGCCAAGGATCTAAGCT
  CCCCAGGCATGCATGCTCCGCCCGTGGTTGCCCCCGATGCTGCAGCCCATGTTGATGCCCCAAGTGGGCT
  GGAGGCAGAGTTGGAGCACCTGCGGCAAGCCCTGGAGGGTGGCCTGGATACTAAGGAAGCCAAAGAAAAG
  TTCCTGCATGAGGTGGTCAAAATGCGAGTTAAGCAAGAGGAAAAGCTGACAGCAGCCCTGCAGGCTAAGC
  GCAGCCTGCACCAGGAGCTAGAATTCTTGCGTGTGGCCAAGAAGGAGAAACTGCGGGAAGCCACAGAGGC
  CAAGCGCAGTTTGAGGAAAGAGATTGAGCGGCTCCGAGCTGAAAACGAGAAGAAGATGAAAGAGGCCAAC
  GAGTCCCGGGTGCGCCTGAAGCGAGAGCTGGAGCAGGCGCGGCAGGTCCGGGTGTGTGACAAGGGCTGCG
  AGGCCGGGCGCCTGCGTGCCAAGTACTCAGCCCAGATTGAGGACCTGCAAGCGAAGCTGCAGCATGCGGA
  GGCCGACCGCGAGCAGCTTCGAGCTGACCTGCTGCGGGAACGTGAGGCCCGCGAGCATCTGGAGAAGGTG
  GTGAGGGAGCTTCAGGAGCAGCTGCGGCCTCGGCCACGGCCTGAGCATCCTGGAGGTGAGAGCAACGCTG
  AGCTGGGTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223989 representing NM_011385
  Red=Cloning site Green=Tags(s)
  
  MEAAAAGRGGFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAATVPAPVPTAAEP
  PPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQIN
  SVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALG
  LELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSAN
  WRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYANKYKRRVPRVSEPPASIRPKTDDTSSQSPASSEKDK
  QSTWLRTLAGSSNKSLGCTHPRQRLSAFRPWSPAVSASEKETSPHLPALIRDSFYSYKSFETAVAPNVAL
  APPTQQKVVNSPPCTTVVSRAPEPLTTCIQPRKRKLTLDTAGAPDMLTPVAAAEEDKDSEAEVEVESREE
  FTSSLSSLSSPSFTSSSSAKDLSSPGMHAPPVVAPDAAAHVDAPSGLEAELEHLRQALEGGLDTKEAKEK
  FLHEVVKMRVKQEEKLTAALQAKRSLHQELEFLRVAKKEKLREATEAKRSLRKEIERLRAENEKKMKEAN
  ESRVRLKRELEQARQVRVCDKGCEAGRLRAKYSAQIEDLQAKLQHAEADREQLRADLLREREAREHLEKV
  VRELQEQLRPRPRPEHPGGESNAELGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011385
• ORF Size: 2181 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011385.2, NP_035515.2
• RefSeq Size: 5481 bp
• RefSeq ORF: 2184 bp
• Locus ID: 20481
• Cytogenetics: 4 86.17 cM
• MW: 80.8 kDa