Adam28 (NM_183366) Mouse Tagged ORF Clone

CAT#: MR223823

Adam28 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 28 (Adam28), transcript variant 2

Product Images

Circular map for MR223823

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adam28
• Synonyms: C130072N01Rik; D430033C21Rik; Dtgn; Dtgn1; eMDCII; MDC-; MDC-L; MDCL; TECADAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223823 representing NM_183366
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAATGGAGTCTTCTGGTAGTCTCTTTCCTTCTTTCTCCAGTTCCAGTAAGTGCAATAAAAGAAC
  TCCCTAAAGCCAAGAAATATGAAGTGGTTTATCCCATAAGACTTCATCCATTGCGTAAAAGAGAGACCCA
  AGAGCCAGAGCCAAAGGAAACATTTGAAACTGAGCTAAGGTACAAAATGACAGTAAATGGAAAGGTTGCT
  GTGCTGTATCTGAAGAAGAACAACAAGCTCCTTGCGCCTGACTACTCGGAAACATACTATAATTCCAGTG
  GAAACAAGGTCACCACAAGCCCGCAAATCATGGATAGCTGTTACTACCAAGGACACATCGTAAATGAGAA
  AGTTTCTGCAGCCAGCATCAGCACCTGTCAAGGACTACGGGGTTACATCAGTCAAGGAGATGAAAAGTAT
  TTTATCGAACCTTTGAGCTCGGAGAACTTGGATGAACAGGCACATGCACTCTTCAAGGACGACTCCAATG
  AAGACCAGGAGAAGAGCAACTGTGGTGTGGATGATGCGCTATGGCTCCAAGGGCTGCATCAGGACGTGGC
  CCTCCCTGCCACCAGGTTGATTAAGTTGAATGATGGGATGGTTCAAGAACCTAAGAAATACATAGAATAT
  TATGTGGTCCTGGATAATGGTGAGTTTAAGAAATACAATAAAAATCTTGCTGAAATACGAAAGATAGTGC
  TTGAGATGGCCAATTACATCAACATGCTTTACAATAAGCTTGATGCCCACGTGGCCTTAGTTGGAGTGGA
  AATCTGGACCGATGGGGATAAAATAAAGATAACACCAGATGCCAACACCACCCTGGAAAACTTCTCTAAG
  TGGAGGGGAAATGATCTGCTAAAACGAAAGCATCATGATATTGCCCAGCTAATCTCATCAACAGACTTTT
  CTGGATCAACAGTGGGTCTAGCCTTCATGTCGTCGATGTGTTCACCTTACCATTCTGTTGGCATTGTTCA
  GGACCACAGTAACTACCATCTTCGAGTCGCAGGAACAATGGCTCATGAAATGGGTCACAATCTTGGCATG
  ATTCATGACTACTTGAGCTGCAAGTGTCCATCTGAAGTCTGTGTAATGGAGCAGTCACTAAGGTTCCATA
  TGCCTACAGACTTCAGCTCCTGCAGTCGTGTCAATTACAAACAGTTTCTTGAAGAAAAATTATCGCATTG
  CCTCTTTAATAGCCCATTGCCATCAGATATCATATCCACCCCAGTCTGTGGGAACCAGTTGTTGGAAATG
  AATGAGGACTGTGACTGTGGCACACCCAAGGAGTGTACTAACAAATGCTGTGATGCAAGGACCTGTAAAA
  TTAAAGCAGGTTTCCAGTGTGCCCTGGGGGAATGCTGTGAGAAATGCCAACTTAAAAAACCTGGGGTTGT
  GTGCAGAGCAGCAAAAGATGAGTGTGATCTGCCTGAAGTGTGTGATGGTAAATCCAGCCACTGCCCAGGT
  GACAGATTCAGAGTCAATGGCTCCCCTTGCCAAAATGGGCATGGTTACTGCTTGAAGGGCAAATGTCCCA
  CCCTGCAGCAGCAGTGCATGGACATGTGGGGTCCAGGAACCAAGGTTGCAAATACATCATGTTACAAGCA
  GAATGAAGGTGGGACAAAGTACGGATACTGTCATGTGGAGAATGGCACACACATGCCCTGCAAAGCAAAA
  GATGCCATGTGTGGGAAATTGTTCTGTGAAGGCGGATCAGGTGATTTGCCCTGGAAAGGACTTACCATAT
  CTTTCCTGACATGTAAATTATTTGATCCTGAAGACACAAGTCAAGGAGTAGACATGGTGGCCAATGGAAC
  CAAGTGTGGAACTAACAAGGTGTGCATTAATGCTGAGTGTGTGGACATGGAGAAGACTTACAAGTCAGCC
  AACTGCTCCTCAAAGTGCAAGGGGCACGCAGTGTGTGACCATGAGCTTCAGTGTCAGTGCAAGGAAGGAT
  GGGCCCCTCCTGACTGCGAGAATTCAGCCACAGTCTTCCACTTCTCCATCGTGGTTGGCGTGCTTTTCCC
  CCTAGCAGTCATATTTGTGGTGGTTGCTATAGTGATCCAGCGCCAAAGTGCCAGAAGGAAGCAGAGGAGA
  GTTCAGAGGCTACCATCCACCAAGGATGCCAAGCTACACAATCAGAAGTGTAGACCCCAAAAGGTGAAGG
  ATGTTCAACCCCAGGAGATGAGTCAGATGAAAAAGCTCCATGTGTCTGATCTGCCCTCTGAAGAGCCGGA
  GCCTCCACCTGATGTCCTAATCACAAAGCCAAATTTCCCACCACCACCAATTCCTGTTTCCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223823 representing NM_183366
  Red=Cloning site Green=Tags(s)
  
  MQQWSLLVVSFLLSPVPVSAIKELPKAKKYEVVYPIRLHPLRKRETQEPEPKETFETELRYKMTVNGKVA
  VLYLKKNNKLLAPDYSETYYNSSGNKVTTSPQIMDSCYYQGHIVNEKVSAASISTCQGLRGYISQGDEKY
  FIEPLSSENLDEQAHALFKDDSNEDQEKSNCGVDDALWLQGLHQDVALPATRLIKLNDGMVQEPKKYIEY
  YVVLDNGEFKKYNKNLAEIRKIVLEMANYINMLYNKLDAHVALVGVEIWTDGDKIKITPDANTTLENFSK
  WRGNDLLKRKHHDIAQLISSTDFSGSTVGLAFMSSMCSPYHSVGIVQDHSNYHLRVAGTMAHEMGHNLGM
  IHDYLSCKCPSEVCVMEQSLRFHMPTDFSSCSRVNYKQFLEEKLSHCLFNSPLPSDIISTPVCGNQLLEM
  NEDCDCGTPKECTNKCCDARTCKIKAGFQCALGECCEKCQLKKPGVVCRAAKDECDLPEVCDGKSSHCPG
  DRFRVNGSPCQNGHGYCLKGKCPTLQQQCMDMWGPGTKVANTSCYKQNEGGTKYGYCHVENGTHMPCKAK
  DAMCGKLFCEGGSGDLPWKGLTISFLTCKLFDPEDTSQGVDMVANGTKCGTNKVCINAECVDMEKTYKSA
  NCSSKCKGHAVCDHELQCQCKEGWAPPDCENSATVFHFSIVVGVLFPLAVIFVVVAIVIQRQSARRKQRR
  VQRLPSTKDAKLHNQKCRPQKVKDVQPQEMSQMKKLHVSDLPSEEPEPPPDVLITKPNFPPPPIPVSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183366
• ORF Size: 2304 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183366.3, NP_899222.1
• RefSeq Size: 2638 bp
• RefSeq ORF: 2307 bp
• Locus ID: 13522
• UniProt ID: Q9JLN6
• Cytogenetics: 14 D1
• MW: 86.3 kDa
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are typically membrane-anchored, although a form of this protein may be secreted. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a mature protein product. This protein may bind to integrins and regulate lymphocyte migration by enhancing cell adhesion. [provided by RefSeq, Aug 2015]