Pou2f1 (NM_198934) Mouse Tagged ORF Clone

CAT#: MR223773

Pou2f1 (Myc-DDK-tagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 4

Product Images

Circular map for MR223773

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pou2f1
• Synonyms: 2810482H01Rik; NF-A1; Oct-1; Oct1; Otf-1; Otf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223773 representing NM_198934
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACTGCAGTGACTGTGTTCTAGACTCAAGAATGAATAATCCATCAGAAACCAATAAATCATCTA
  TGGAGAGTGAAGATGCCAGCACAGGCACACAAACCAATGGTCTGGACTTTCAGAAACAGCCCGTGCCCGT
  TGGAGGAGCGATCTCCACAGCCCAGGCCCAGGCCTTCCTCGGACATCTTCACCAGGTCCAGCTAGCTGGG
  ACAAGTTTACAGGCTGCTGCTCAGTCTTTAAATGTACAGTCTAAATCCAGTGAAGAGTCGGGAGATTCGC
  AGCAGTCGAGCCAGCCTTCTTCCCAGCCGCCTTCAGTGCAGTCAGCCATTCCCCAGACCCAGCTAATGCT
  GGCTGGGGGACAGATAACTGGGCTCACGTTGACCCCAGCCCAGCAACAGTTACTGCTACAGCAGGCGCAG
  GCCCAGGCCCAGCTCCTGGCCGCTGCAGTGCAGCAACACTCCGCCAGCCAACAGCACAGTGCTGCTGGGG
  CCACCATCTCAGCCTCCGCCGCCACACCCATGACGCAGATCCCCCTGTCTCAGCCCATACAGATTGCACA
  GGATCTTCAACAATTGCAACAGCTTCAGCAGCAAAATCTCAACTTGCAACAGTTTGTGTTGGTGCACCCA
  ACCACCAACCTGCAACCAGCACAGTTTATCATCTCACAGACCCCCCAGGGCCAGCAGGGTCTCCTGCAAG
  CGCAAAATCTTTTAACGCAACTACCTCAGCAAAGCCAAGCCAACCTCCTACAGCCACAGCCAAGCATCAC
  CCTCACGTCCCAGCCTACCACCCCAACTCGCACAATAGCAGCAGCCTCAGTTCAGACACTTCCACAGAGC
  CAGTCAACACCAAAGCGAATTGACACTCCCAGCTTGGAGGAGCCCAGTGACCTTGAGGAGCTTGAGCAGT
  TTGCCAAGACTTTCAAACAAAGACGAATCAAACTTGGATTCACTCAGGGTGATGTTGGGCTCGCTATGGG
  GAAATTATATGGAAATGACTTCAGCCAAACCACCATCTCTCGCTTTGAAGCCTTGAACCTCAGCTTTAAG
  AACATGTGCAAGTTAAAGCCCCTTTTAGAGAAGTGGCTAAATGATGCAGAGAACCTCTCATCTGATTCTA
  CAGCATCTAGCCCAAGTGCTTTGAATTCTCCAGGATTGGGGGCTGAGGGCTTGAATCGTAGGAGGAAAAA
  ACGCACCAGCATAGAGACCAACATCCGTGTGGCCTTAGAGAAGAGTTTCATGGAGAATCAAAAGCCTACC
  TCGGAAGACATCACCTTGATTGCTGAACAGCTCAATATGGAAAAGGAGGTGATTCGTGTTTGGTTTTGTA
  ACCGCCGCCAGAAGGAGAAAAGAATCAACCCGCCCAGCAGTGGTGGGACCAGCAGCTCACCTATCAAAGC
  AATTTTCCCCAGCCCAGCCTCATTGGTGGCAACCACTCCAAGCCTTGTGACAAGCAGTACGGCAACTACC
  CTCACAGTCAACCCTGTCCTCCCTTTAACCAGTGCTGCTGTGACTAATCTCTCTCTTACAGATCAAGATC
  TTAGAAGAGGATGCAGCTGGGAAGTGCTTAGGAGTCTACCAGACAGAGTCACCACCACAGCAGGCACTAC
  AGACTCGACGTCCAACAACAACACGGCCACGGTGATTTCCACAGCACCCCCTGCTTCCTCAGCAGTCACA
  TCCCCTTCCTTGAGTCCCTCTCCCTCTGCCTCGGCCTCCACCTCAGAGGCCTCCAGTGCCAGTGAGACCA
  ACACGACACAGACCACCTCCACGCCTCTTCCCTCCCCTCTCGGAGCCAGCCAGGTGATGGTGACCACGCC
  CGGCTTACAGACAGCAGCCGCCGCTCTCCAAGGAGCGGCACAGTTGCCAGCAAACGCCAGTCTTGCTGCT
  ATGGCTGCTGCTGCGGGACTCAGCCCAGGCCTCATGGCACCCTCACAGTTTGCTGCTGGAGGTGCCTTAC
  TCAGTCTCAGTCCGGGGACTCTGGGCAGTGCTCTCAGCCCAGCCCTAATGAGCAACAGTACACTGGCAAC
  GATTCAAGCTCTTGCTTCTAGTGGCTCTCTTCCAATAACGTCTCTGGATGCAACTGGGAACCTGGTATTT
  GCCAATGCAGGAGGAGCCCCGAACATCGTGACTGCACCTCTGTTCCTGAACCCTCAGAACCTCTCTCTGC
  TCACCAGCAACCCAGTAAGCTTGGTTTCTGCCGCTGCAGCCTCCACAGGGAACTCTGCACCTACAGCCAG
  CCTTCATGCCTCCTCCACCTCAACTGAGTCCATCCAGAGCTCTCTGTTCACAGTCGCCTCTGCCAGTGGG
  CCTGCTTCCACCACCACAGCTGCCTCCAAGGCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223773 representing NM_198934
  Red=Cloning site Green=Tags(s)
  
  MLDCSDCVLDSRMNNPSETNKSSMESEDASTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAG
  TSLQAAAQSLNVQSKSSEESGDSQQSSQPSSQPPSVQSAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQ
  AQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHP
  TTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQPQPSITLTSQPTTPTRTIAAASVQTLPQS
  QSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFK
  NMCKLKPLLEKWLNDAENLSSDSTASSPSALNSPGLGAEGLNRRRKKRTSIETNIRVALEKSFMENQKPT
  SEDITLIAEQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPASLVATTPSLVTSSTATT
  LTVNPVLPLTSAAVTNLSLTDQDLRRGCSWEVLRSLPDRVTTTAGTTDSTSNNNTATVISTAPPASSAVT
  SPSLSPSPSASASTSEASSASETNTTQTTSTPLPSPLGASQVMVTTPGLQTAAAALQGAAQLPANASLAA
  MAAAAGLSPGLMAPSQFAAGGALLSLSPGTLGSALSPALMSNSTLATIQALASSGSLPITSLDATGNLVF
  ANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASTGNSAPTASLHASSTSTESIQSSLFTVASASG
  PASTTTAASKAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198934
• ORF Size: 2346 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198934.3, NP_945152.2
• RefSeq Size: 2684 bp
• RefSeq ORF: 2349 bp
• Locus ID: 18986
• Cytogenetics: 1 73.21 cM
• MW: 81.3 kDa
• Gene Summary: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity).[UniProtKB/Swiss-Prot Function]