G2e3 (NM_001167964) Mouse Tagged ORF Clone

CAT#: MR223648

G2e3 (Myc-DDK-tagged) - Mouse G2/M-phase specific E3 ubiquitin ligase (G2e3), transcript variant 3

Product Images

Circular map for MR223648

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: G2e3
• Synonyms: 6030408C04Rik; 9030416F18; AW046379; D930034K21Rik; mKIAA1333
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223648 representing NM_001167964
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGAAAATAAACCTGATAATTCACAAAGTCTTGCTTGTGTTTTCTGCCGAAAAAATGATGACTGTC
  CCAATAAATACGGAGAAAAGAAAACATATGAAAAATGGAATTTTAGTGTCCACTACTACTGCTTGTTAAT
  GTCAAGTGGAATTTGGCAGAGAGGTAAAGAAGAAGAAGGAGTTTATGGCTTCCTAATAGAAGACATCAGG
  AAGGAAGTCCAGAGAGCCTCCAAACTGAAGTGTACAGTTTGCAAGAAGAATGGTGCTTCAATTGGATGTG
  TGGTACCCACGTGTAAACGAAGTTATCACTTGCCTTGTGGACTTCAGAAAGAATGTATTTTCCAGTTCAC
  TGACAATTTTGCGTCATTTTGCTGGAAACATCGACCCGTTCAAGCAATTACATCTAATAAATATAGTAGT
  TCTTTACCATGTACTATCTGTTTGGAATTTGTCGAGCCTATTCCTACTTATAACATACTACAAAGTCCCT
  GTTGTAAGAATGCTTGGTTTCATAGAGACTGTTTACAGGTCCAAGCAATAAATGCAGGAGTGTTTTTCTT
  TAGATGTACACTATGCAATAATACAGACATCTTTCAGAAGGAGATGTTGAGAATGGGAATTCATATTCCT
  GAAAAAGATGCATCCTGGGAATTAGAAGAAAACGCGTATCAAGAGCTGCTGCAGAGCCACGACCGCTGTG
  ATATTCGAAGATGTCACTGCAAAAAAGGCCGAGACTACAACGAACCTAACAGCAAATGGGAAGTAAAGCG
  GTGTCAGAGTTGTGGTTCTAGTGGAACACACTTAGCCTGTTCCTCACTACAGTCTTGGGAACAAAATTGG
  GAGTGTTTGGACTGTAGAAGAATTACCTACACTTCAGATTTCCAAAAGGCCCCCAAACACCCATTAGCCA
  ACTCTACTAATGTGACAGTTACTGACTGCTTGTTGGAAGAGTCATCATCTAAGCTCCCCAGACAGTCAAC
  TGTAGCCCAGCATAAAGAACTACTGAGTTTATTTTTATCTGTATTTACAGCTGTGAAAGAGAATCTTTAC
  TATGAAGTTGGCAAAATGCTTGCCATTTCTTTAGTTCATGGTGGTCCTTCGCCTGGTTTCTTTTCTGAAA
  CCTTGTTTAACTGCCTTGCTTATGGACCAGAAAATACTCTACCAACTTTGGATGATGTATCAGACATTGA
  TGTTGCACAGATTATAATCAAGATAGATTCTGCAACTGATTTAAACATTTTAAATTCAGTAATAAGCCAA
  CACTATAACTACTTAGAGGTCAGTGGATGTTTAAGACTTACGACATCATTAAGTGATAAATTCATGTTGG
  TAAAAGACATCCTTTTCTACCATGTAATTAATAGAGTCAAGGCACCTTTTGAGAGTTTTAAACAGGGTCT
  GAAAACGCTTGGTGTTTTAGAGAAAATTCAAACTTACCCAGAAGCATTTTATAAGATCCTATGTCATAAA
  CCTGAGAATCTCTCTGCAAAAAACCTCAGTGACCTCTTCACAATTCACTCGGTAGCGGACGTACAGACTT
  TGAGGTTTTGGAACAGTTACTTAAAGGCTATAGAGGATGGTAAATCTGCAACAACGATGGAAGATATTCT
  TATTTTTGCAACTGGCTGTAGTTCTGTCCCACCAACTGGATTTAAACCCTCTCTGTCAGTTGAATGTCTA
  CATGTGGATTTTCCTGTTGCAGACAAATATAGAAACCATTTAGTTCTACCAGCCACCAACACGTATGAAG
  AGTTCCAAGAAAATATGGATTTCACCATAAGAGACACTCTAAGATTAGAAAAGGAAGAAAGGTCTCACAT
  ACTACCTCGGACATTAAATGTTTCTTCTAATGAAGAAATGCTTATT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223648 representing NM_001167964
  Red=Cloning site Green=Tags(s)
  
  MNENKPDNSQSLACVFCRKNDDCPNKYGEKKTYEKWNFSVHYYCLLMSSGIWQRGKEEEGVYGFLIEDIR
  KEVQRASKLKCTVCKKNGASIGCVVPTCKRSYHLPCGLQKECIFQFTDNFASFCWKHRPVQAITSNKYSS
  SLPCTICLEFVEPIPTYNILQSPCCKNAWFHRDCLQVQAINAGVFFFRCTLCNNTDIFQKEMLRMGIHIP
  EKDASWELEENAYQELLQSHDRCDIRRCHCKKGRDYNEPNSKWEVKRCQSCGSSGTHLACSSLQSWEQNW
  ECLDCRRITYTSDFQKAPKHPLANSTNVTVTDCLLEESSSKLPRQSTVAQHKELLSLFLSVFTAVKENLY
  YEVGKMLAISLVHGGPSPGFFSETLFNCLAYGPENTLPTLDDVSDIDVAQIIIKIDSATDLNILNSVISQ
  HYNYLEVSGCLRLTTSLSDKFMLVKDILFYHVINRVKAPFESFKQGLKTLGVLEKIQTYPEAFYKILCHK
  PENLSAKNLSDLFTIHSVADVQTLRFWNSYLKAIEDGKSATTMEDILIFATGCSSVPPTGFKPSLSVECL
  HVDFPVADKYRNHLVLPATNTYEEFQENMDFTIRDTLRLEKEERSHILPRTLNVSSNEEMLI
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167964
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167964.1, NP_001161436.1
• RefSeq Size: 6352 bp
• RefSeq ORF: 1869 bp
• Locus ID: 217558
• UniProt ID: Q5RJY2
• Cytogenetics: 12 B3
• MW: 71.5 kDa
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Required for prevention of apoptotic death in early embryogenesis.[UniProtKB/Swiss-Prot Function]