G2e3 (NM_001167964) Mouse Untagged Clone

CAT#: MC219864

G2e3 (untagged) - Mouse G2/M-phase specific E3 ubiquitin ligase (G2e3), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: G2e3
• Synonyms: 6030408C04Rik; 9030416F18; AW046379; D930034K21Rik; mKIAA1333
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219864 representing NM_001167964
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGAAAATAAACCTGATAATTCACAAAGTCTTGCTTGTGTTTTCTGCCGAAAAAATGATGACTGTC
  CCAATAAATACGGAGAAAAGAAAACATATGAAAAATGGAATTTTAGTGTCCACTACTACTGCTTGTTAAT
  GTCAAGTGGAATTTGGCAGAGAGGTAAAGAAGAAGAAGGAGTTTATGGCTTCCTAATAGAAGACATCAGG
  AAGGAAGTCCAGAGAGCCTCCAAACTGAAGTGTACAGTTTGCAAGAAGAATGGTGCTTCAATTGGATGTG
  TGGTACCCACGTGTAAACGAAGTTATCACTTGCCTTGTGGACTTCAGAAAGAATGTATTTTCCAGTTCAC
  TGACAATTTTGCGTCATTTTGCTGGAAACATCGACCCGTTCAAGCAATTACATCTAATAAATATAGTAGT
  TCTTTACCATGTACTATCTGTTTGGAATTTGTCGAGCCTATTCCTACTTATAACATACTACAAAGTCCCT
  GTTGTAAGAATGCTTGGTTTCATAGAGACTGTTTACAGGTCCAAGCAATAAATGCAGGAGTGTTTTTCTT
  TAGATGTACACTATGCAATAATACAGACATCTTTCAGAAGGAGATGTTGAGAATGGGAATTCATATTCCT
  GAAAAAGATGCATCCTGGGAATTAGAAGAAAACGCGTATCAAGAGCTGCTGCAGAGCCACGACCGCTGTG
  ATATTCGAAGATGTCACTGCAAAAAAGGCCGAGACTACAACGAACCTAACAGCAAATGGGAAGTAAAGCG
  GTGTCAGAGTTGTGGTTCTAGTGGAACACACTTAGCCTGTTCCTCACTACAGTCTTGGGAACAAAATTGG
  GAGTGTTTGGACTGTAGAAGAATTACCTACACTTCAGATTTCCAAAAGGCCCCCAAACACCCATTAGCCA
  ACTCTACTAATGTGACAGTTACTGACTGCTTGTTGGAAGAGTCATCATCTAAGCTCCCCAGACAGTCAAC
  TGTAGCCCAGCATAAAGAACTACTGAGTTTATTTTTATCTGTATTTACAGCTGTGAAAGAGAATCTTTAC
  TATGAAGTTGGCAAAATGCTTGCCATTTCTTTAGTTCATGGTGGTCCTTCGCCTGGTTTCTTTTCTGAAA
  CCTTGTTTAACTGCCTTGCTTATGGACCAGAAAATACTCTACCAACTTTGGATGATGTATCAGACATTGA
  TGTTGCACAGATTATAATCAAGATAGATTCTGCAACTGATTTAAACATTTTAAATTCAGTAATAAGCCAA
  CACTATAACTACTTAGAGGTCAGTGGATGTTTAAGACTTACGACATCATTAAGTGATAAATTCATGTTGG
  TAAAAGACATCCTTTTCTACCATGTAATTAATAGAGTCAAGGCACCTTTTGAGAGTTTTAAACAGGGTCT
  GAAAACGCTTGGTGTTTTAGAGAAAATTCAAACTTACCCAGAAGCATTTTATAAGATCCTATGTCATAAA
  CCTGAGAATCTCTCTGCAAAAAACCTCAGTGACCTCTTCACAATTCACTCGGTAGCGGACGTACAGACTT
  TGAGGTTTTGGAACAGTTACTTAAAGGCTATAGAGGATGGTAAATCTGCAACAACGATGGAAGATATTCT
  TATTTTTGCAACTGGCTGTAGTTCTGTCCCACCAACTGGATTTAAACCCTCTCTGTCAGTTGAATGTCTA
  CATGTGGATTTTCCTGTTGCAGACAAATATAGAAACCATTTAGTTCTACCAGCCACCAACACGTATGAAG
  AGTTCCAAGAAAATATGGATTTCACCATAAGAGACACTCTAAGATTAGAAAAGGAAGAAAGGTCTCACAT
  ACTACCTCGGACATTAAATGTTTCTTCTAATGAAGAAATGCTTATTTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001167964
• Insert Size: 1869 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167964.1, NP_001161436.1
• RefSeq Size: 6352 bp
• RefSeq ORF: 1869 bp
• Locus ID: 217558
• UniProt ID: Q5RJY2
• Cytogenetics: 12 B3
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Required for prevention of apoptotic death in early embryogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) is missing an internal coding exon and uses an alternate acceptor splice site at another coding exon compared to variant 1. However, it retains the reading frame and encodes a shorter isoform (3) lacking an internal protein segment compared to isoform 1.