Tas1r1 (NM_031867) Mouse Tagged ORF Clone

CAT#: MR223642

Tas1r1 (Myc-DDK-tagged) - Mouse taste receptor, type 1, member 1 (Tas1r1)

Product Images

Circular map for MR223642

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tas1r1
• Synonyms: Gpr70; T1r1; TR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223642 representing NM_031867
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTCTGGGCAGCTCACCTGCTGCTCAGCCTGCAGCTGGCCGTTGCTTACTGCTGGGCTTTCAGCT
  GCCAAAGGACAGAATCCTCTCCAGGTTTCAGCCTCCCTGGGGACTTCCTCCTGGCAGGCCTGTTCTCCCT
  CCATGCTGACTGTCTGCAGGTGAGACACAGACCTCTGGTGACAAGTTGTGACAGGTCTGACAGCTTCAAC
  GGCCATGGCTATCACCTCTTCCAAGCCATGCGGTTCACCGTTGAGGAGATAAACAACTCCACAGCTCTGC
  TTCCCAACATCACCCTGGGGTATGAACTGTATGACGTGTGCTCAGAGTCTTCCAATGTCTATGCCACCCT
  GAGGGTGCTCGCCCAGCAAGGGACAGGCCACCTAGAGATGCAGAGAGATCTTCGCAACCACTCCTCCAAG
  GTGGTGGCACTCATTGGGCCTGATAACACTGACCACGCTGTCACCACTGCTGCCCTGCTGAGCCCTTTTC
  TGATGCCCCTGGTCAGCTATGAGGCGAGCAGCGTGATCCTCAGTGGGAAGCGCAAGTTCCCGTCCTTCTT
  GCGCACCATCCCCAGCGATAAGTACCAGGTGGAAGTCATAGTGCGGCTGCTGCAGAGCTTCGGCTGGGTC
  TGGATCTCGCTCGTTGGCAGCTATGGTGACTACGGGCAGCTGGGCGTACAGGCGCTGGAGGAGCTGGCCA
  CTCCACGGGGCATCTGCGTCGCCTTCAAGGACGTGGTGCCTCTCTCCGCCCAGGCGGGTGACCCAAGGAT
  GCAGCGCATGATGCTGCGTCTGGCTCGAGCCAGGACCACCGTGGTCGTGGTCTTCTCTAACCGGCACCTG
  GCTGGAGTGTTCTTCAGGTCTGTGGTGCTGGCCAACCTGACTGGCAAAGTGTGGATCGCCTCCGAAGACT
  GGGCCATCTCCACGTACATCACCAATGTGCCCGGGATCCAGGGCATTGGGACGGTGCTGGGGGTGGCCAT
  CCAGCAGAGACAAGTCCCTGGCCTGAAGGAGTTTGAAGAGTCCTATGTCCAGGCAGTGATGGGTGCTCCC
  AGAACTTGCCCAGAGGGGTCCTGGTGCGGCACTAACCAGCTGTGCAGGGAGTGTCACGCTTTCACGACAT
  GGAACATGCCCGAGCTTGGAGCCTTCTCCATGAGCGCTGCCTACAATGTGTATGAGGCTGTGTATGCTGT
  GGCCCACGGCCTCCACCAGCTCCTGGGATGTACCTCTGGGACCTGTGCCAGAGGCCCAGTCTACCCCTGG
  CAGCTTCTTCAGCAGATCTACAAGGTGAATTTCCTTCTACATAAGAAGACTGTAGCATTCGATGACAAGG
  GGGACCCTCTAGGTTATTATGACATCATCGCCTGGGACTGGAATGGACCTGAATGGACCTTTGAGGTCAT
  TGGTTCTGCCTCACTGTCTCCAGTTCATCTAGACATAAATAAGACAAAAATCCAGTGGCACGGGAAGAAC
  AATCAGGTGCCTGTGTCAGTGTGTACCAGGGACTGTCTCGAAGGGCACCACAGGTTGGTCATGGGTTCCC
  ACCACTGCTGCTTCGAGTGCATGCCCTGTGAAGCTGGGACATTTCTCAACACGAGTGAGCTTCACACCTG
  CCAGCCTTGTGGAACAGAAGAATGGGCCCCTGAGGGGAGCTCAGCCTGCTTCTCACGCACCGTGGAGTTC
  TTGGGGTGGCATGAACCCATCTCTTTGGTGCTATTAGCAGCTAACACGCTATTGCTGCTGCTGCTGATTG
  GGACTGCTGGCCTGTTTGCCTGGCGTCTTCACACGCCTGTTGTGAGGTCAGCTGGGGGTAGGCTGTGCTT
  CCTCATGCTGGGTTCCTTGGTAGCTGGGAGTTGCAGCCTCTACAGCTTCTTCGGGAAGCCCACGGTGCCC
  GCGTGCTTGCTGCGTCAGCCCCTCTTTTCTCTCGGGTTTGCCATTTTCCTCTCCTGTCTGACAATCCGCT
  CCTTCCAACTGGTCATCATCTTCAAGTTTTCTACCAAGGTACCCACATTCTACCACACTTGGGCCCAAAA
  CCATGGTGCCGGAATATTCGTCATTGTCAGCTCCACGGTCCATTTGTTCCTCTGTCTCACGTGGCTTGCA
  ATGTGGACCCCACGGCCCACCAGGGAGTACCAGCGCTTCCCCCATCTGGTGATTCTTGAGTGCACAGAGG
  TCAACTCTGTGGGCTTCCTGGTGGCTTTCGCACACAACATCCTCCTCTCCATCAGCACCTTTGTCTGCAG
  CTACCTGGGTAAGGAACTGCCGGAGAACTATAACGAAGCCAAATGTGTCACCTTCAGCCTGCTCCTCCAC
  TTCGTATCCTGGATCGCTTTCTTCACCATGTCCAGCATTTACCAGGGCAGCTACCTACCCGCGGTCAATG
  TGCTGGCAGGGCTGGCCACTCTGAGTGGCGGCTTCAGCGGCTATTTCCTCCCTAAATGCTACGTGATTCT
  CTGCCGTCCAGAACTCAACAACACAGAACACTTTCAGGCCTCCATCCAGGACTACACGAGGCGCTGCGGC
  ACTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223642 representing NM_031867
  Red=Cloning site Green=Tags(s)
  
  MLFWAAHLLLSLQLAVAYCWAFSCQRTESSPGFSLPGDFLLAGLFSLHADCLQVRHRPLVTSCDRSDSFN
  GHGYHLFQAMRFTVEEINNSTALLPNITLGYELYDVCSESSNVYATLRVLAQQGTGHLEMQRDLRNHSSK
  VVALIGPDNTDHAVTTAALLSPFLMPLVSYEASSVILSGKRKFPSFLRTIPSDKYQVEVIVRLLQSFGWV
  WISLVGSYGDYGQLGVQALEELATPRGICVAFKDVVPLSAQAGDPRMQRMMLRLARARTTVVVVFSNRHL
  AGVFFRSVVLANLTGKVWIASEDWAISTYITNVPGIQGIGTVLGVAIQQRQVPGLKEFEESYVQAVMGAP
  RTCPEGSWCGTNQLCRECHAFTTWNMPELGAFSMSAAYNVYEAVYAVAHGLHQLLGCTSGTCARGPVYPW
  QLLQQIYKVNFLLHKKTVAFDDKGDPLGYYDIIAWDWNGPEWTFEVIGSASLSPVHLDINKTKIQWHGKN
  NQVPVSVCTRDCLEGHHRLVMGSHHCCFECMPCEAGTFLNTSELHTCQPCGTEEWAPEGSSACFSRTVEF
  LGWHEPISLVLLAANTLLLLLLIGTAGLFAWRLHTPVVRSAGGRLCFLMLGSLVAGSCSLYSFFGKPTVP
  ACLLRQPLFSLGFAIFLSCLTIRSFQLVIIFKFSTKVPTFYHTWAQNHGAGIFVIVSSTVHLFLCLTWLA
  MWTPRPTREYQRFPHLVILECTEVNSVGFLVAFAHNILLSISTFVCSYLGKELPENYNEAKCVTFSLLLH
  FVSWIAFFTMSSIYQGSYLPAVNVLAGLATLSGGFSGYFLPKCYVILCRPELNNTEHFQASIQDYTRRCG
  TT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031867
• ORF Size: 2526 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031867.2, NP_114073.1
• RefSeq Size: 2814 bp
• RefSeq ORF: 2529 bp
• Locus ID: 110326
• UniProt ID: Q99PG6
• Cytogenetics: 4 82.83 cM
• MW: 93.9 kDa
• Gene Summary: Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate) and also to most of the 20 standard L-amino acids, but not to their D-enantiomers or other compounds. Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.[UniProtKB/Swiss-Prot Function]