Vmn1r174 (NM_207548) Mouse Tagged ORF Clone

CAT#: MR223580

Vmn1r174 (Myc-DDK-tagged) - Mouse vomeronasal 1 receptor 174 (Vmn1r174)

Product Images

Circular map for MR223580

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vmn1r174
• Synonyms: V1rd22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223580 representing NM_207548
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCTCTTAATAAAGCCCTGAAAACCACTGAGGAAGTGGCCCTTCAAATCATCTTGCTTTGCCAGG
  TTGGTATTGCAGCTGTGGCCAACATTCTTCTGCTTGTCCATAATTTATCTCCTAACTTTGCTGGCCCTCA
  ACTGAGACCAAAACATGTCATTCTTGCCCACATTACTGTGGGCAATCTCTTGATTATCCTCCTCACTGGG
  TTTCCAAGCAATATGACAGTTTTTGTTCCAAGGAATCCTCTAAATGACCTCAACTGTAAGCTTGAGTACT
  TCATTCGCCTGGTGGCTCGAAGTGCAAACATGTGTACTACTTGTATCCTGAGCTCTTACCAGTTTATCGT
  TCTTGTTCCTGGTAAATGGAGTAGAGTGATACTCAGAGGAAATACCTCCACATTTGTGCACTATTCTTGT
  AATAGTTGTTGGATTATCAGTGTCTTAAATAATGTCTACATCCCTATAAAAATCAGTGGTCCATGGAACA
  CAGACAATAACACTGACCCGAACAGCAAGTGGGTATGTTCTAACTCTGGTTTCGAGTTAGTCATGGTCTT
  CTTACGATTTGTCCACGATGCCATATTCATCAGCATCATGGTATGGACCAGTGTTTCCATGATGCTTCTC
  TTGTATAGACATCACCAAAGCCTGAAACACATTCACAGGTCCAATGGGAGCCTGGGACTTCAACCAGAGG
  CCAGAGCAGCAAACACAATTCTCATGCTGATGATCACATTTGTATGCTTTTATCTTCTCGATTGTATTTG
  TATTTTCTTTCACATTTCTTTTGTGCATTTTCGTGTATGGTTGAGGCATGTCAGTGAAATTTTGGCTGCA
  AGTTTCCCCACCATTTCTCCCTTAATGTTGATCGTTAGGGGTCCTAGAAAAATATCAGCCAAAGGGATAA
  ACACAGAAAATGCTTTTATTAACAGTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223580 representing NM_207548
  Red=Cloning site Green=Tags(s)
  
  MSALNKALKTTEEVALQIILLCQVGIAAVANILLLVHNLSPNFAGPQLRPKHVILAHITVGNLLIILLTG
  FPSNMTVFVPRNPLNDLNCKLEYFIRLVARSANMCTTCILSSYQFIVLVPGKWSRVILRGNTSTFVHYSC
  NSCWIISVLNNVYIPIKISGPWNTDNNTDPNSKWVCSNSGFELVMVFLRFVHDAIFISIMVWTSVSMMLL
  LYRHHQSLKHIHRSNGSLGLQPEARAANTILMLMITFVCFYLLDCICIFFHISFVHFRVWLRHVSEILAA
  SFPTISPLMLIVRGPRKISAKGINTENAFINSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207548
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207548.2, NP_997431.2
• RefSeq Size: 942 bp
• RefSeq ORF: 942 bp
• Locus ID: 404291
• Cytogenetics: 7 A3
• MW: 35.2 kDa