Tmem27 (NM_020626) Mouse Tagged ORF Clone

CAT#: MR223526

Tmem27 (Myc-DDK-tagged) - Mouse transmembrane protein 27 (Tmem27)

Product Images

Circular map for MR223526

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem27
• Synonyms: 0610008J07Rik; NX-17; NX17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223526 representing NM_020626
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTGGGCGCTCTTTTTCCTGGTGACTACTATTCACGCTGAACTCTGCCATCCAGATGCAGAAAATG
  CCTTTAAAGTAAGACTTAGCATCAGAGCAGCTCTTGGAGATAAAGCATATGTCTGGGACACGGATCAAGA
  ATACCTCTTCAGAGCAATGGTGGCATTCTCCATGAGAAAAGTTCCCAACAGAGAAGCAACAGAAATTTCC
  CATGTCCTGCTTTGCAACATAACCCAGAGGGTGTCGTTCTGGTTTGTGGTCACGGACCCTTCCAACAATT
  ATACTCTTCCTGCAGCTGAAGTACAGTCGGCCATAAGAAAGAATAGGAACCGGATCAACAGTGCATTTTT
  TCTGGATGACCATACTCTGGAATTTTTAAAAATTCCTTCTACTCTTGCTCCCCCGATGGAGCCATCTGTG
  CCCGTCTGGATTATTGTATTCGGTGTGATATTTTGCATTGTTACAGTTGCAATTGCACTACTGGTTCTAT
  CTGGAATCCGGCAACGCAGAAGGAACAACAAAGGACCACCTGGAGTGGAAGATGCAGAAGACAAGTGTGA
  AAACATCATCACAATAGAAAATGGCATCCCTTGTGATCCCTTGGACATGAAGGGAGGGCACATTAATGAT
  GGCTTCTTGACAGAGGATGAGCGCCTCACCCCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223526 representing NM_020626
  Red=Cloning site Green=Tags(s)
  
  MLWALFFLVTTIHAELCHPDAENAFKVRLSIRAALGDKAYVWDTDQEYLFRAMVAFSMRKVPNREATEIS
  HVLLCNITQRVSFWFVVTDPSNNYTLPAAEVQSAIRKNRNRINSAFFLDDHTLEFLKIPSTLAPPMEPSV
  PVWIIVFGVIFCIVTVAIALLVLSGIRQRRRNNKGPPGVEDAEDKCENIITIENGIPCDPLDMKGGHIND
  GFLTEDERLTPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020626
• ORF Size: 666 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020626.2, NP_065651.1
• RefSeq Size: 1262 bp
• RefSeq ORF: 669 bp
• Locus ID: 57394
• UniProt ID: Q9ESG4
• Cytogenetics: X F5
• MW: 25.5 kDa
• Gene Summary: Plays an important role in amino acid transport by acting as binding partner of amino acid transporters SLC6A18 and SLC6A19, regulating their trafficking on the cell surface and their activity (PubMed:17167413). Regulator of SNARE complex function (PubMed:16330323). Stimulator of beta cell replication (PubMed:16330324).[UniProtKB/Swiss-Prot Function]