Nfil3 (NM_017373) Mouse Tagged ORF Clone

CAT#: MR223366

Nfil3 (Myc-DDK-tagged) - Mouse nuclear factor, interleukin 3, regulated (Nfil3)

Product Images

Circular map for MR223366

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfil3
• Synonyms: AV225605; E4BP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223366 representing NM_017373
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGCAGCTGAGAAAAATGCAGACCATCAAAAAGGAGCCCGCACCCCTAGATCCTACCAGCAGCTCAGACA
  AGATGCTGCTGCTGAACTCTGCCTTAGCTGAGGTGGCCGAGGACCTAGCCTCAGGTGAAGATTTGCTCCT
  GAACGAAGGGAGCATGGGGAAAAACAAATCCTCGGCGTGTCGGAGAAAACGGGAATTCATTCCGGACGAG
  AAGAAAGACGCCATGTATTGGGAGAAACGGCGGAAAAACAACGAAGCTGCCAAAAGATCTCGGGAGAAGC
  GCCGCCTCAATGACCTGGTTTTGGAGAACAAGCTGATCGCCCTGGGAGAAGAAAATGCCACTTTAAAAGC
  TGAGCTGCTCTCCCTGAAATTAAAGTTTGGTTTAATTAGCTCCACGGCGTATGCCCAAGAAATCCAGAAA
  CTCAGTAATTCCACAGCTGTCTACTTTCAGGACTACCAGACATCCAAGGCTGCCGTGAGCTCTTTTGTGG
  ACGAGCATGAGCCTGCGATGGTAGCCGGAAGTTGCATCTCAGTCATCAAGCACTCTCCCCAGAGCTCGCT
  CTCCGATGTGTCAGAGGTGTCCTCGGTGGAGCACACTCAGGAAAGCCCCGCACAGGGAGGCTGCCGGAGC
  CCTGAGAACAAGTTCCCTGTGATCAAGCAGGAGCCCGTGGAGTTGGAGAGCTTTGCCAGGGAGGCCAGGG
  AGGAGCGGGGCACGTATTCCACCTCCATCTACCAGAGCTACATGGGAAGCTCTTTCTCCACTTACTCCCA
  CTCCCCACCCCTCTTGCAGGTCCATGGGTCCACTAGCAACTCCCCAAGAACCTCAGAGGCCGATGAGGGT
  GTAGTGGGCAAGTCTTCTGATGGGGAAGACGAACAACAGGTCCCTAAGGGCCCCATCCATTCTCCAGTGG
  AGCTGCAACGGGTCCACGCCACGGTGGTGAAGGTTCCGGAAGTGAACCCTTCTGCCTTACCGCACAAGCT
  TCGGATTAAAGCCAAGGCCATGCAGGTCAAAGTGGAGGCTTTGGACAGCGAGTTTGAAGGCATGCAGAAA
  CTCTCTTCACCCGCCGATGCGATCGCCAAAAGACATTTTGACCTGGAGAAACATGGAACCTCGGGTATGG
  CCCATTCCTCCCTCCCTCCTTTCTCAGTGCAGGTGACGAACATTCAAGATTGGTCCCTCAAATCGGAACA
  CTGGCATCACAAAGAACTGAGCAGCAAAACTCAGAGTAGCTTCAAAACAGGTGTGGTGGAAGTCAAAGAC
  GGTGGCTATAAGGTTTCCGAAGCTGAGAATTTGTATTTGAAGCAGGGAATAGCAAACTTATCTGCAGAGG
  TGGTCTCGCTCAAGAGATTCATAGCCACACAACCGATCTCGGCTTCGGACTCCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223366 representing NM_017373
  Red=Cloning site Green=Tags(s)
  
  MQLRKMQTIKKEPAPLDPTSSSDKMLLLNSALAEVAEDLASGEDLLLNEGSMGKNKSSACRRKREFIPDE
  KKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELLSLKLKFGLISSTAYAQEIQK
  LSNSTAVYFQDYQTSKAAVSSFVDEHEPAMVAGSCISVIKHSPQSSLSDVSEVSSVEHTQESPAQGGCRS
  PENKFPVIKQEPVELESFAREAREERGTYSTSIYQSYMGSSFSTYSHSPPLLQVHGSTSNSPRTSEADEG
  VVGKSSDGEDEQQVPKGPIHSPVELQRVHATVVKVPEVNPSALPHKLRIKAKAMQVKVEALDSEFEGMQK
  LSSPADAIAKRHFDLEKHGTSGMAHSSLPPFSVQVTNIQDWSLKSEHWHHKELSSKTQSSFKTGVVEVKD
  GGYKVSEAENLYLKQGIANLSAEVVSLKRFIATQPISASDSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017373
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017373.3, NP_059069.1
• RefSeq Size: 2019 bp
• RefSeq ORF: 1389 bp
• Locus ID: 18030
• UniProt ID: O08750
• Cytogenetics: 13 27.68 cM
• MW: 50.9 kDa
• Gene Summary: The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses Per1 and Per2 expression and therefore plays a role in the regulation of circadian rhythm. [provided by RefSeq, Feb 2014]