Eif4g1 (NM_001005331) Mouse Tagged ORF Clone

CAT#: MR223361

Eif4g1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 2

Product Images

Circular map for MR223361

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif4g1
• Synonyms: E030015G23Rik; eIF4GI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223361 representing NM_001005331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAAGCTCCCCAGCCCACAGGCCCCCCACCCGCCAGGTCCCCTGGACTCCCACAGCCAGCGTTTC
  CCCCGGGGCAGACTGCACCGGTGGTGTTTAGCACGCCTCAAGCGACACAAATGAACACGCCTTCTCAGCC
  CCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCTAGCAGTGCAGCCTCCCGAGTGCAGAGTGCAGCC
  CCTGCCCGTCCTGGCCCAGCTCCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGA
  TCTCCTACTCAGCCTCCCAAGGAGCCTACTATATCCCTGGACAGGGGCGTTCCACATATGTTGTCCCAAC
  ACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAAGCCCTACCGAGTTTGGGACC
  TATGCTGGAGCCTATTACCCAGCCCAAGGTGTGCAGCAGTTTCCTGCTAGTGTGGCTCCTGCCCCAGTTT
  TGATGAACCAGCCACCCCAGATTGCTCCTAAGAGGGAACGGAAAACTATCCGAATTCGAGACCCAAACCA
  AGGAGGGAAGGATATCACAGAAGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACTCCTCCCCAG
  ACGGGAGGCAGTCTGGAGCCTCAACCCAATGGGGAGTCGCCTCAGGTTGCTGTCATTATCCGGCCAGATG
  ACCGGTCGCAGGGAGCAGCCATTGGGGGGCGGCCAGGACTGCCTGGCCCAGAGCATAGCCCTGGCACAGA
  ATCTCAGCCTTCGTCGCCTTCTCCAACCCCATCACCACCCCCAATTTTGGAGCCGGGGTCTGAGTCTAAT
  CTTGGAGTCCTCTCTATTCCTGGGGACACTATGACAACAGGGATGATACCAATGTCTGTAGAAGAATCGA
  CCCCTATCTCTTGTGAAACTGGGGAGCCGTATTGCCTCTCTCCAGAACCCACTCTTGCCGAACCCATACT
  GGAAGTAGAAGTGACACTCAGCAAACCCATTCCAGAATCTGAGTTCTCTTCCAGTCCTCTCCAGGTTTCC
  ACGGCCCTGGTGCCTCACAAGGTTGAAACTCATGAGCCCAATGGCGTGATCCCATCTGAGGATCTGGAAC
  CAGAGGTGGAGTCAAGCACAGAGCCAGCTCCTCCCCCTCTATCACCTTGTGCTTCTGAATCGCTCGTGCC
  CATTGCTCCAACTGCCCAGCCTGAGGAACTGCTCAACGGAGCCCCCTCACCACCAGCTGTGGATTTAAGC
  CCAGTCAGTGAGCCAGAGGAACAGGCCAAGAAGGTTTCATCAGCAGCACTGGCCAGCATTCTCTCTCCTG
  CTCCACCTGTGGCTCCTTCAGATACTTCTCCTGCTCAGGAGGAAGAAATGGAAGAAGATGACGATGACGA
  AGAAGGTGGAGAAGCTGAGAGTGAGAAGGGAGGAGAGGACGTCCCCCTTGACAGTACTCCTGTCCCAGCC
  CAGCTGTCTCAGAATTTAGAGGTGGCAGCAGCTACCCAGGTGGCAGTGTCTGTGCCAAAGAGGAGACGGA
  AAATTAAAGAGCTAAATAAGAAGGAGGCTGTAGGAGACCTTCTAGATGCCTTCAAGGAGGTGGACCCAGC
  AGTACCAGAGGTAGAGAATCAGCCTCCCACTGGCAGCAACCCAAGCCCAGAGTCTGAGGGCAGCATGGTG
  CCCACACAGCCTGAGGAAACAGAAGAAACCTGGGACTCTAAGGAAGACAAAATTCATAATGCTGAGAACA
  TCCAGCCTGGGGAGCAGAAGTATGAGTACAAGTCAGATCAGTGGAAGCCTCTAAACCTTGAAGAAAAGAA
  GCGTTATGACAGAGAATTCCTGCTGGGCTTTCAGTTCATCTTTGCCAGCATGCAGAAGCCAGAAGGATTG
  CCCCATATCACTGATGTGGTGCTGGACAAGGCCAATAAAACACCACTTCGGCAACTGGATCCCTCCAGAC
  TACCTGGCATAAACTGTGGCCCAGATTTCACTCCATCTTTTGCCAATCTTGGCCGACCAGCCCTCAGCAA
  CCGTGGGCCCCCGAGGGGTGGGCCAGGTGGGGAGCTGCCCCGAGGGCCGGCTGGCCTGGGACCCAGGCGC
  TCTCAGCAGGGCCCACGAAAGGAAACTCGCAAGATCATTTCCTCAGTGATAATGACTGAAGACATAAAAC
  TAAATAAAGCAGAGAAGGCTTGGAAACCCAGTAGCAAACGGACAGCAGCTGATAAGGATCGAGGGGAAGA
  GGATGCTGATGGAAGCAAGACCCAGGACCTGTTCCGCAGGGTACGCTCCATCCTGAATAAGCTGACACCC
  CAGATGTTTCAGCAGCTGATGAAGCAGGTGACACAGCTGGCCATTGACACGGAGGAACGCCTCAAAGGAG
  TTATTGACCTCATCTTTGAGAAAGCCATTTCAGAGCCCAACTTCTCTGTGGCTTATGCCAACATGTGCCG
  CTGCCTCATGGCGCTGAAAGTGCCCACTACAGAAAAGCCAACAGTAACTGTGAATTTTCGAAAACTGTTG
  TTAAATCGGTGCCAGAAGGAATTTGAGAAAGACAAAGATGATGATGAAGTTTTTGAAAAAAAGCAAAAAG
  AGATGGATGAAGCTGCTACGGCAGAAGAACGGGGACGCCTGAAAGAAGAGCTAGAAGAAGCCCGGGACAT
  AGCTAGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTATTCAAGTTGAAGATGTTAACAGAA
  GCAATAATGCATGACTGTGTGGTCAAGCTACTTAAGAACCATGATGAGGAGTCCCTGGAATGCCTCTGCC
  GCCTCCTCACCACTATAGGCAAAGACCTGGACTTTGCAAAAGCCAAGCCTCGAATGGATCAGTATTTCAA
  CCAAATGGAAAAAATCATTAAAGAAAAGAAGACCTCATCTCGCATCCGTTTTATGCTGCAGGATGTACTC
  GATCTGCGGCAGAGTAATTGGGTGCCACGCCGAGGGGATCAGGGTCCTAAGACTATTGATCAAATTCACA
  AGGAGGCTGAGATGGAAGAGCACCGAGAACATATTAAAGTACAGCAGCTCATGGCCAAGGGCAGCGACAA
  ACGTCGGGGTGGCCCTCCAGGCCCGCCTATCAACCGTGGCCTTCCACTTGTAGATGATGGTGGCTGGAAT
  ACAGTCCCCATTAGCAAAGGCAGTCGCCCCATTGACACCTCACGGCTCACCAAGATCACAAAGCCTGGTT
  CCATTGATTCTAACAACCAGCTTTTTGCACCTGGAGGACGACTGAGTTGGGGCAAGGGCAGCAGTGGGGG
  CTCAGGAGCCAAGCCCTCAGACACAGCATCGGAAGCTACTCGTCCAGCTACCTTGAATCGCTTTTCTGCT
  CTTCAACAAACATTACCCGCAGAGAACACAGATAACAGACGTGTTGTACAGAGGAGTAGCTTGAGCCGGG
  AACGAGGTGAGAAAGCTGGGGACCGGGGAGACCGACTAGAGCGGAGTGAGCGGGGAGGTGACCGTGGGGA
  CCGACTTGATCGTGCCAGAACACCTGCCACCAAGCGAAGTTTTAGCAAAGAAGTGGAGGAGCGAAGTAGA
  GAGCGGCCATCCCAGCCTGAGGGACTCCGCAAGGCAGCTAGCCTCACAGAGGATCGTGGTCGGGATCCTG
  TGAAGCGGGAAGCCACTCTACCTCCAGTGAGCCCTCCAAAGGCTGCGCTGTCTGTGGATGAGGTGGAGAA
  GAAATCTAAGGCCATCATTGAGGAATATCTCCATCTCAATGACATGAAGGAGGCAGTACAGTGTGTCCAG
  GAGCTGGCTTCACCCTCCTTGCTCTTCATCTTCGTACGGCTTGGTATCGAGTCCACGCTGGAGCGTAGTA
  CCATTGCTCGTGAGCATATGGGGCGACTACTGCACCAACTGCTCTGTGCGGGGCACCTCTCTACTGCCCA
  GTACTATCAAGGGCTGTATGAAACACTAGAATTGGCTGAGGACATGGAAATTGATATCCCTCATGTATGG
  CTTTACCTGGCAGAACTGATTACACCTATTCTCCAGGAAGATGGGGTGCCCATGGGAGAGCTCTTCAGGG
  AAATTACGAAGCCTCTGAGACCCATGGGCAAAGCTACTTCTTTATTGCTGGAGATCCTGGGTCTCTTATG
  CAAGAGCATGGGTCCCAAAAAGGTGGGGATGCTGTGGCGAGAGGCTGGGCTGAGCTGGAGGGAATTTCTA
  GCAGAGGGCCAAGACGTTGGCTCATTTGTGGCTGAAAAGAAGGTGGAATATACCTTGGGAGAAGAATCTG
  AAGCTCCTGGCCAGAGGACACTTGCCTTTGAGGAGCTTCGTAGGCAGCTAGAGAAGCTGCTGAAGGACGG
  CGGCAGTAATCAGCGTGTGTTCGACTGGATAGACGCCAACCTAAATGAGCAGCAGATAGCATCCAATACA
  TTAGTTCGAGCCCTCATGACAACTGTCTGTTATTCTGCAATTATCTTTGAGACTCCTCTCCGAGTGGATG
  TTCAAGTGTTGAAAGTGCGAGCAAGACTGCTGCAGAAATACCTGTGTGATGAGCAGAAGGAGCTACAAGC
  ACTCTATGCTCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTGCCAACCTGCTTCGGATGTTCTTTGAT
  GCTCTATATGATGAGGACGTGGTGAAGGAAGACGCCTTCTACAGCTGGGAGAGCAGCAAGGATCCTGCTG
  AACAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCATTCTTCAATTGGCTTCGTGAGGCTGAGGA
  CGAGGAGTCTGATCACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223361 representing NM_001005331
  Red=Cloning site Green=Tags(s)
  
  MNKAPQPTGPPPARSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAA
  PARPGPAPHVYPAGSQVMMIPSQISYSASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGT
  YAGAYYPAQGVQQFPASVAPAPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQ
  TGGSLEPQPNGESPQVAVIIRPDDRSQGAAIGGRPGLPGPEHSPGTESQPSSPSPTPSPPPILEPGSESN
  LGVLSIPGDTMTTGMIPMSVEESTPISCETGEPYCLSPEPTLAEPILEVEVTLSKPIPESEFSSSPLQVS
  TALVPHKVETHEPNGVIPSEDLEPEVESSTEPAPPPLSPCASESLVPIAPTAQPEELLNGAPSPPAVDLS
  PVSEPEEQAKKVSSAALASILSPAPPVAPSDTSPAQEEEMEEDDDDEEGGEAESEKGGEDVPLDSTPVPA
  QLSQNLEVAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEVDPAVPEVENQPPTGSNPSPESEGSMV
  PTQPEETEETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGL
  PHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPAGLGPRR
  SQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTP
  QMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLL
  LNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTE
  AIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVL
  DLRQSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPINRGLPLVDDGGWN
  TVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDTASEATRPATLNRFSA
  LQQTLPAENTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSR
  ERPSQPEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQCVQ
  ELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVW
  LYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFL
  AEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELRRQLEKLLKDGGSNQRVFDWIDANLNEQQIASNT
  LVRALMTTVCYSAIIFETPLRVDVQVLKVRARLLQKYLCDEQKELQALYALQALVVTLEQPANLLRMFFD
  ALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFNWLREAEDEESDHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005331
• ORF Size: 4779 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005331.1, NP_001005331.1
• RefSeq Size: 5460 bp
• RefSeq ORF: 4782 bp
• Locus ID: 208643
• Cytogenetics: 16 B1
• MW: 175.8 kDa
• Gene Summary: This gene encodes a member of the eukaryotic translation initiation factors (eIF) that play important roles in translation initiation by mediating recruitment of additional initiation factors and providing a scaffold for ribosome/mRNA-bridging. Along with eIF4A and eIF4E, the encoded protein forms the eIF4F complex that bridges the 5' UTR with the polyadenylated 3' UTR resulting in mRNA circularization, enhanced translation initiation and mRNA stability. Through its association with eIF3, the encoded protein mediates recruitment of the 43S pre-initiation complex to mRNA. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes for this gene have been identified on chromosomes 2 and 13. [provided by RefSeq, Jan 2015]