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Col4a3 (NM_007734) Mouse Tagged ORF Clone
CAT#: MR223337
- TrueORF®
Col4a3 (Myc-DDK-tagged) - Mouse collagen, type IV, alpha 3 (Col4a3)
ORF Plasmid: tGFP
"NM_007734" in other vectors (2)
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Col4a3 |
| Synonyms | alpha3(IV); [a]3(IV) |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223337 representing NM_007734
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCCAAGACTGCTCCAAGGTTCCTGGTGTTCCTGCTGCTTACCCTGCTGCTACTCCTGGCTGCCT CTCCTGTGGCTAGCAAGGGCTGTGTCTGCAAAGGCAAAGGACAATGCCTCTGCGCGGGGACCAAAGGGGA GAAGGGGGAGAAAGGGGTTCCTGGTTCCCCTGGATTTCCTGGCCAGAAAGGATTTCCAGGTCCTGAAGGC TTGCCTGGACCACAGGGACCCAAGGGCTCCCCAGGACTTCCGGGACTCACTGGCCCCAAAGGCATCAGGG GAATAACTGGATTACCAGGGTTTGCAGGTCCTCCTGGACTTCCAGGCCTCCCAGGCCACCCTGGGCCTCG TGGGCTGGCTGGTTTACCAGGATGCAACGGATCTAAGGGTGAACAAGGATTCCCGGGCTTTCCCGGCACA CCAGGCTATGCAGGGCTCCCAGGTCCTGATGGCTTGAAAGGACAAAAGGGTGAGCCTGCTCAAGGAGAAG ACAGGGGATTCAATGGAAAAGGTGACCCTGGGCCTCCAGGGGTTCCAGGCTTCCAGGGTTTTCCGGGACT CCCAGGTTTTCCAGGGCCTGCCGGTCCACCAGGACCTCCGGGATTTTTTGGTTTACCAGGAGCAATGGGA CCCAGAGGACCCAAAGGCCACATGGGCGATAGCGTGATAGGACAAAAAGGAGAAAGGGGTATGAAAGGAT TAACAGGACCTCCTGGGCCACCAGGAACAGTGATTTTTACACTCACCCAGCCATACAACAAATCGGACTT CAAGGGAGAGAAAGGAGATGAGGGAGAGAGGGGTGAACCCGGACCTCCTGGACCCTCGGGGCCACCTGGA GACTCCTATGGATCAGAAAAGGGTGCGCCTGGAGAGCCTGGTCCTCGGGGCAAACCTGGAAAAGATGGTG CCCCTGGCTTCCCTGGCACTGAGGGAGCCAAAGGCAACAGGGGCTTTCCTGGGTTGAGGGGTGAAGCTGG CATTAAGGGAAGGAAAGGAGACATTGGCCCCCCAGGATTTCCTGGTCCAACAGAATATTATGACGCATAC CTGGAAAAGGGAGAGAGAGGAATGCCAGGCCTTCCTGGGCCCAAAGGAGCCCGTGGCCCACAGGGTCCAA GTGGTCCCCCTGGAGTCCCTGGAAGTCCTGGACTGTCAAGGCCTGGCCTCAGAGGACCCATTGGATGGCC AGGCTTGAAAGGGAGTAAAGGAGAGAGAGGACCCCCTGGAAAAGACACTGTGGGCCCTCCTGGACCCCTG GGATGTCCTGGCTCACCAGGTCCACCAGGCCCTCCAGGACCTCCAGGATGTCCAGGTGACATTGTTTTTA AATGTTCCCCTGGTGAGCATGGAATGCCAGGTGACACAGGACCTCCAGGAGTTCCAGGACTTGATGGACC CAAAGGAGAACCAGGCAGTCCATGCACCGAGTGTCACTGCTTCCCAGGGCCCCCTGGAGTCCCAGGATTT CCAGGATTAGATGGTATCAAAGGAATCCCAGGAGGACGAGGTGTGCCTGGTCTGAAGGGAAACCCAGGGT CCCCAGGAAGTGCAGGTCTCCCAGGATTTGCAGGATTCCCAGGTGACCAAGGGCATCCAGGACTTAAAGG GGACAAAGGGGATACACCTCTACCCTGGGGGCAAGTGGGTAATCCAGGTGATCCTGGACTCAGAGGCCTG CCCGGGAGAAAGGGCTTCGATGGAACTCCTGGAGGTCCAGGAGCGAAAGGACCACCAGGACCTCAGGGCG AACCGGCCCTGAGTGGAAGGAAAGGGGACCAAGGACCTCCAGGACCTCCTGGATTCCCTGGACCCCCAGG ACCTGCAGGACCAGCTGGACCACCAGGCTATGGACCTCAAGGAGAGCCAGGTCCAAAGGGAGCCCAAGGA GTCCCCGGCGTCCTGGGACCACCTGGAGAAGCCGGTCTTAAAGGAGAACCCAGTACATCAACTCCAGATC TAGGTCCCCCAGGCCCTCCAGGGCCCCCTGGTCAGGCTGGCCCCAGAGGTCTACCTGGTTTGCCTGGACC GGTGGGAAAATGTGATCCTGGTCTTCCTGGACCTGATGGTGAGCCAGGAATTCCAGAAGCTGGATGCCCA GGGCCTCCAGGACCTAAGGGAAATCAAGGTTTCCCAGGGACAAAAGGATCACCTGGTTGTCCTGGGGAAA TGGGAAAGCCTGGCCGTCCTGGAGAACCAGGCATCCCAGGAGCCAAGGGAGAACCATCCGTAGGCAGGCC TGGGAAACCAGGGAAACCAGGCTTTCCAGGAGAAAGAGGCAATGCTGGGGAAAATGGAGATATTGGACTC CCTGGGCTTCCAGGCCTCCCTGGAACTCCAGGAAGAGGCGGGCTTGACGGGCCTCCAGGAGACCCAGGAC AGCCTGGATCACCTGGAGCAAAAGGATCCCCAGGAAGGTGCATACCAGGACCCAGGGGCACCCAAGGACT TCCCGGCTTGAATGGATTGAAAGGGCAACCAGGTCGAAGAGGTGATACCGGGCCAAAGGGAGACCCTGGC ATCCCAGGCATGGACAGGTCTGGGGTCCCCGGAGATCCTGGACCACCAGGAACACCAGGTTGTCCTGGTG AGATGGGACCACCTGGACAAAAAGGATATCCGGGAGCTCCAGGATTCCCAGGACCACCAGGCGAGAAAGG AGAGGTTGGAATGATGGGCTATCCTGGAACCACTGGCCCTCCGGGGCTTCCTGGGAAACCAGGCTCACAG GGGCAGCGAGGTAGCCTCGGAATCCCAGGAATGAAAGGGGAAAAAGGACGCCCAGGAGCCAAAGGCGAAC GAGGAGAGAAAGGAAAACCAGGGCCTTCTCAAACAACACTCTTAAAGGGAGACAAAGGAGAGCCTGGACT TAAAGGATTTGTTGGGAATCCAGGTGAGAAAGGAAACAGAGGCAACCCAGGGTTACCAGGTCCGAAAGGC CTCGAGGGATTGCCTGGGCTACCAGGTCCTCCAGGCCCTAGAGGAGATACGGGAAGCAGAGGAAATCCTG GAAGACCAGGACCACACGGCATGCCAGGAAGCATGGGGATCATGGGGGTGCCAGGCCCTAAAGGGAGAAA AGGAACTTCAGGGCTTCCAGGTCTAGCTGGAAGACCAGGCCTGACAGGGATCCATGGTCCCCAAGGAGAT AAGGGGGAGCCGGGTTATTCAGAAGGTGCAAGACCAGGACCACCAGGACCAAAGGGCGATCCAGGATTGC CAGGTGACAAAGGAAAGAAAGGAGAAAGAGGCGTACCTGGGCCACCTGGACAATCGGGGCCTGCCGGACC TGATGGAGCCCCTGGGAGTCCTGGGAGTCCTGGTCACCCAGGAAAACCAGGTCCTGCTGGTGATTTGGGT CTGAAAGGACAGAAAGGCTTCCCAGGCCCTCCAGGAAGCACTGGCCCTCCAGGCCCTCCAGGACTCCCAG GACTTCCTGGGCCAATGGGTATGAGAGGTGACCAAGGACGTGATGGAATTCCTGGTCCTCCAGGAGAAAA AGGAGAAACAGGCTTGCTGGGGGCCTACCCAGGCCCAAAAGGGAGCCCTGGTGTACCAGGTGCCAAAGGA GACAGGGGAGTTCCAGGCTTGTCTGGCCTTCCCGGCAGGAAGGGGGTTATGGGGGATGTTGGACCCCAAG GACCCCCAGGCACTGCTGGACTCCCAGGGCCACCAGGTCTACCTGGGGCAATTATCCCTGGCCCCAAAGG AGACAGAGGTCTTCCTGGCTTAAGAGGAAATCCAGGTGAGCCAGGTCCCCCTGGACCTCCAGGACCTATC GGAAAAGGCATAAAAGGTGACAAAGGATTTATGGGCCCACCTGGCCCCAAAGGCCTGCCTGGAACTGTAG GGGACATGGGCCCACCAGGTTTCCCGGGAGCACCAGGTACCCCCGGTCTTCCCGGTGTCAGAGGTGATCC AGGATTCCCTGGATTTCCAGGCATAAAAGGAGAAAAGGGTAACCCTGGATTTCTTGGGCCAATTGGACAT CCAGGACCAGTTGGGCCCAAAGGACCACCAGGTCCACGTGGAAAACCTGGCACTCTTAAGGTCATCTCTC TTCCGGGAAGCCCAGGGCCACCTGGTGTACCTGGACAGCCAGGAATGAAAGGAGACCCTGGACCCCTGGG ACTGCCAGGAATCCCAGGACCCTGTGGGCCAAGAGGTAAACCAGGCAAGGATGGGAAACCAGGAACTCCA GGACCAGCTGGTACAAAGGGCAACAAGGGCTTGAAAGGACAGCAAGGCCCACCTGGTCTGGATGGATTGC CAGGCTTAAAGGGAAATCCTGGTGACAGGGGAACGCCGGCCACTGGTACAAGAATGCGAGGCTTCATCTT CACCCGACACAGTCAAACCACGGCCATTCCTTCATGCCCTGAAGGAACACAGCCACTCTATAGTGGGTTT TCTCTTCTTTTTGTACAAGGAAACAAACGTGCACATGGACAAGACCTAGGTACTCTGGGCAGCTGCCTGC AGCGATTCACCACAATGCCGTTCTTATTCTGTAACATCAATAATGTATGTAACTTTGCATCACGAAATGA TTATTCATACTGGCTGTCAACACCAGCTCTGATGCCAATGGACATGGCTCCAATTAGTGGCAGAGCTCTC GAACCCTATATTAGCAGATGCACCGTCTGTGAAGGTCCAGCAATGGCCATAGCTGTTCACAGTCAAACTA CTGCTATCCCTCCGTGTCCCCAGGACTGGGTTTCTCTCTGGAAAGGTTTTTCTTTCATTATGTTCACAAG TGCAGGCTCTGAGGGTGCTGGACAAGCACTTGCCTCGCCTGGCTCCTGCCTGGAAGAATTCCGAGCCAGT CCATTTATAGAATGCCATGGACGAGGGACATGTAACTACTACTCAAACTCCTACAGTTTCTGGCTGGCTT CGCTGAACCCAGAAAGAATGTTCAGAAAACCTATTCCATCAACTGTGAAAGCTGGAGACTTAGAGAAAAT CATAAGCCGCTGTCAGGTGTGCATGAAGAAAAGACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223337 representing NM_007734
Red=Cloning site Green=Tags(s) MHSKTAPRFLVFLLLTLLLLLAASPVASKGCVCKGKGQCLCAGTKGEKGEKGVPGSPGFPGQKGFPGPEG LPGPQGPKGSPGLPGLTGPKGIRGITGLPGFAGPPGLPGLPGHPGPRGLAGLPGCNGSKGEQGFPGFPGT PGYAGLPGPDGLKGQKGEPAQGEDRGFNGKGDPGPPGVPGFQGFPGLPGFPGPAGPPGPPGFFGLPGAMG PRGPKGHMGDSVIGQKGERGMKGLTGPPGPPGTVIFTLTQPYNKSDFKGEKGDEGERGEPGPPGPSGPPG DSYGSEKGAPGEPGPRGKPGKDGAPGFPGTEGAKGNRGFPGLRGEAGIKGRKGDIGPPGFPGPTEYYDAY LEKGERGMPGLPGPKGARGPQGPSGPPGVPGSPGLSRPGLRGPIGWPGLKGSKGERGPPGKDTVGPPGPL GCPGSPGPPGPPGPPGCPGDIVFKCSPGEHGMPGDTGPPGVPGLDGPKGEPGSPCTECHCFPGPPGVPGF PGLDGIKGIPGGRGVPGLKGNPGSPGSAGLPGFAGFPGDQGHPGLKGDKGDTPLPWGQVGNPGDPGLRGL PGRKGFDGTPGGPGAKGPPGPQGEPALSGRKGDQGPPGPPGFPGPPGPAGPAGPPGYGPQGEPGPKGAQG VPGVLGPPGEAGLKGEPSTSTPDLGPPGPPGPPGQAGPRGLPGLPGPVGKCDPGLPGPDGEPGIPEAGCP GPPGPKGNQGFPGTKGSPGCPGEMGKPGRPGEPGIPGAKGEPSVGRPGKPGKPGFPGERGNAGENGDIGL PGLPGLPGTPGRGGLDGPPGDPGQPGSPGAKGSPGRCIPGPRGTQGLPGLNGLKGQPGRRGDTGPKGDPG IPGMDRSGVPGDPGPPGTPGCPGEMGPPGQKGYPGAPGFPGPPGEKGEVGMMGYPGTTGPPGLPGKPGSQ GQRGSLGIPGMKGEKGRPGAKGERGEKGKPGPSQTTLLKGDKGEPGLKGFVGNPGEKGNRGNPGLPGPKG LEGLPGLPGPPGPRGDTGSRGNPGRPGPHGMPGSMGIMGVPGPKGRKGTSGLPGLAGRPGLTGIHGPQGD KGEPGYSEGARPGPPGPKGDPGLPGDKGKKGERGVPGPPGQSGPAGPDGAPGSPGSPGHPGKPGPAGDLG LKGQKGFPGPPGSTGPPGPPGLPGLPGPMGMRGDQGRDGIPGPPGEKGETGLLGAYPGPKGSPGVPGAKG DRGVPGLSGLPGRKGVMGDVGPQGPPGTAGLPGPPGLPGAIIPGPKGDRGLPGLRGNPGEPGPPGPPGPI GKGIKGDKGFMGPPGPKGLPGTVGDMGPPGFPGAPGTPGLPGVRGDPGFPGFPGIKGEKGNPGFLGPIGH PGPVGPKGPPGPRGKPGTLKVISLPGSPGPPGVPGQPGMKGDPGPLGLPGIPGPCGPRGKPGKDGKPGTP GPAGTKGNKGLKGQQGPPGLDGLPGLKGNPGDRGTPATGTRMRGFIFTRHSQTTAIPSCPEGTQPLYSGF SLLFVQGNKRAHGQDLGTLGSCLQRFTTMPFLFCNINNVCNFASRNDYSYWLSTPALMPMDMAPISGRAL EPYISRCTVCEGPAMAIAVHSQTTAIPPCPQDWVSLWKGFSFIMFTSAGSEGAGQALASPGSCLEEFRAS PFIECHGRGTCNYYSNSYSFWLASLNPERMFRKPIPSTVKAGDLEKIISRCQVCMKKRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007734 |
| ORF Size | 5007 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007734.2, NP_031760.2 |
| RefSeq Size | 8609 bp |
| RefSeq ORF | 5010 bp |
| Locus ID | 12828 |
| UniProt ID | Q9QZS0 |
| Cytogenetics | 1 C5 |
| MW | 161.7 kDa |
| Gene Summary | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.[UniProtKB/Swiss-Prot Function] |
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