Eda (NM_001177942) Mouse Tagged ORF Clone

CAT#: MR223119

Eda (Myc-DDK-tagged) - Mouse ectodysplasin-A (Eda), transcript variant 7

Product Images

Circular map for MR223119

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eda
• Synonyms: Ed1; Eda-A1; Eda-A2; EDA1; HED; Ta; tabby; Tnlg7c; XLHED
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223119 representing NM_001177942
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTACCCAGAGGTAGAGCGCAGGGAACCCCTGCCTGCGGCAGCGCCAAGGGAGCGGGGCAGCCAGG
  GCTGCGGCTGTCGCGGGGCCCCTGCTCGGGCGGGCGAAGGGAACAGCTGCCGGCTCTTCCTGGGTTTCTT
  TGGCCTCTCGCTGGCCCTCCACCTGCTGACGCTGTGCTGCTACCTAGAGTTGCGGTCCGAATTGCGGCGG
  GAACGGGGAACCGAGTCCCGCCTCGGTGGCCCGGGTGCTCCTGGCACCTCTGGCACCCTAAGCAGCCCTG
  GGAGCCTCGACCCGGTGGGTCCCATCACCCGCCACCTGGGGCAGCCGTCCTTTCAACAGCAGCCTTTGGA
  ACCGGGAGAAGATCCACTCCCCCCTGACTCCCAGGACCGGCACCAGATGGCCCTCCTGAATTTCTTCTTT
  CCTGATGAAAAGGCATATTCTGAAGAGGAAAGTAGGCGTGTTCGCCGCAATAAGAGAAGCAAAAGTGGTG
  AAGGAGCAGATGGTCCTGTTAAAAACAAGAAAAAGGGAAAGAAGGCAGGGCCACCTGGGCCCAACGGCCC
  CCCAGGACCTCCAGGACCTCCGGGACCCCAGGGACCTCCAGGGATTCCAGGAATTCCTGGGATTCCAGGA
  ACAACTGTTATGGGACCACCTGGCCCACCTGGCCCTCCTGGTCCTCAAGGACCCCCTGGCCTCCAAGGAC
  CTTCTGGTGCTGCTGATAAAACTGGAACTCGGGAAAATCAGCCAGCTGTGGTGCATCTGCAGGGCCAAGG
  GTCAGCAATTCAAGTCAAAAATGATCTTTCAGGTGGAGTGCTCAATGACTGGTCTCGCATCACTATGAAC
  CCTAAGGTGTTTAAACTACATCCCCGCAGCGGGGAGCTGGAGGTCTACTACATCAACTTCACTGACTTTG
  CCAGCTACGAGGTGGTGGTGGATGAGAAGCCCTTCCTGCAGTGCACCCGCAGCATTGAGACAGGGAAGAC
  CAACTACAACACTTGCTATACTGCAGGCGTGTGCCTCCTCAAGGCCAGGCAGAAAATCGCCGTGAAGATG
  GTGCACGCTGACATCTCTATCAATATGAGCAAGCACACCACCTTCTTCGGGGCCATCAGGCTGGGCGAAG
  CCCCTGCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223119 representing NM_001177942
  Red=Cloning site Green=Tags(s)
  
  MGYPEVERREPLPAAAPRERGSQGCGCRGAPARAGEGNSCRLFLGFFGLSLALHLLTLCCYLELRSELRR
  ERGTESRLGGPGAPGTSGTLSSPGSLDPVGPITRHLGQPSFQQQPLEPGEDPLPPDSQDRHQMALLNFFF
  PDEKAYSEEESRRVRRNKRSKSGEGADGPVKNKKKGKKAGPPGPNGPPGPPGPPGPQGPPGIPGIPGIPG
  TTVMGPPGPPGPPGPQGPPGLQGPSGAADKTGTRENQPAVVHLQGQGSAIQVKNDLSGGVLNDWSRITMN
  PKVFKLHPRSGELEVYYINFTDFASYEVVVDEKPFLQCTRSIETGKTNYNTCYTAGVCLLKARQKIAVKM
  VHADISINMSKHTTFFGAIRLGEAPAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177942
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177942.1, NP_001171413.1
• RefSeq Size: 4913 bp
• RefSeq ORF: 1134 bp
• Locus ID: 13607
• UniProt ID: O54693
• Cytogenetics: X 43.59 cM
• MW: 40.4 kDa
• Gene Summary: Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R. Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R (By similarity). May also play a role in cell adhesion (PubMed:10534613).[UniProtKB/Swiss-Prot Function]