Wdr70 (NM_001081402) Mouse Tagged ORF Clone

CAT#: MR223110

Wdr70 (Myc-DDK-tagged) - Mouse WD repeat domain 70 (Wdr70)

Product Images

Circular map for MR223110

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr70
• Synonyms: 4833422F06Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223110 representing NM_001081402
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACTCGGGAACCAGCGAAGTGACAGGCGCAGACACGGCGGGACCCGACCCTCAGCTGGCGGTCA
  CCATGGGCTTCACAGGATTCGGGAAGAAAGCTCGCACATTTGACTTGGAGGCCATGTTCGAGCAAACTCG
  AAGGACAGCGGTGGAAAGAAGCCGGAAAACACTGGAAGCAAGAGAAAAAGAGGAAGAAATGAACAGAGAA
  AAAGAATTAAGAAAACAAATTGAAGATATGGAGCCAGCACCGTCAAGCTCGAGTGCAGCCAGAGAGCGCT
  CACAGTCATCATGCAGGGACACAAGCAGCAGTGACAGTGAGAGTGACGACAGTTCTGATTCATCTGACGA
  TGAGCTAATCGGCCCTCCTTTACCCCCTAAAATGGTAGGAGAATCCGTAACTACTGTGGACGAAGGCACC
  CTTGGCCCTTTACCTCCACCTCTGTGTGAAGAAGGAGAAGATGATGATGATGACGAGTTAGACGATGAAG
  GAGAAGAGGATAACCCTGTCCAGAGGATTCCTGACTCGCATGAGATAACACTGAGGCATGGCACTAAAAC
  AGTTTCTGCTCTGGGTCTGGACCCCTCAGGTGCCCGTTTGGTCACTGGAGGATATGATTACGATGTTAAA
  TTTTGGGATTTTGCTGGAATGGATGCTTCTTTCAAGGCATTTCGATCCCTTCAACCTTGTGAATGCCATC
  AGATCAAGTCGTTACAGTACAGTAACACAGGAGACATGATTCTTGTTGTTTCTGGAAGCTCACAGGCCAA
  GGTTATTGACAGAGATGGTTTTGAAGTCATGGAGTGTATAAAGGGAGACCAGTATATCGTAGACATGGCC
  AACACCAAGGGCCACACAGCAATGCTTCATACTGGCTCATGGCATCCCAAAATAAAAGGAGAATTTATGA
  CTTGCTCAAATGATGCTACTGTGAGGCTCTGGGAAGTTGAAAATCCAAAGAAGCAAAAAAGTGTTTTTAA
  ACCTCGGACAATGCAGGGTAAAAAGGTCATTCCCACGACGTGCACGTACAGCAGAGACGGGAACCTTGTG
  GCCGCTGCCTGCCAGAACGGAAGCATTCAGATCTGGGACCGGAACCTGACAGTTCACCCTAAATTCCACT
  ACAAGCAGGCTCACGACCCTGGCACAGACACGTCTTGTGTGGCTTTTTCCTATGATGGTAATGTCCTTGC
  CTCTCGTGGAGGCGATGATACATTAAAATTATGGGATGTCCGGCAATTTAATAAGCCATTATTTTCAGCC
  TCAGACCTTCCTACCTTGTTCCCAATGACCGACTGCTGCTTCAGCCCTGACGACAAACTCATAGTTACCG
  GTACCTCTGTCCAAAGGGGGTGTGGCAGTGGCAAACTTGTCTTCTTGGAGCGCAGGACTTTCCAAAGGGT
  CTATGAAATCCACATCACAGATGCGAGTGTGGTCCGCTGCCTGTGGCATCCAAAGCTGAACCAGATCATG
  GTTGGAACTGGAAATGGACTGGCAAAAGTGTACTACGACCCCAACAAGAGTCAGAGGGGAGCAAAACTAT
  GTGTGGTTAAAACCCAGAGGAAGGCAAAACAAGCTGAGACACTAACCCAGGACTACATCATCACCCCTCA
  TGCCTTGCCCATGTTCCGTGAGCCCCGCCAGCGAAGTACGAGGAAACAGCTGGAGAAGGACAGACTGGAC
  CCCCTGAAGTCCCACAAACCAGAGCCTCCTGTAGCAGGCCCAGGTCGTGGTGGCCGAGTTGGAACCCACG
  GGGGCACCCTGTCCTCCTACATTGTGAAGAACATCGCTTTGGACAAGACCGACGACAGCAATCCCCGGGA
  AGCCATTTTGCGCCATGCCAAAGCAGCAGAAGATAACCCATATTGGGTTTCGCCAGCATATTCCAAAACT
  CAGCCAAAAACCATGTTTGCCCAAGTGGAGTCTGATGATGAAGAGTCAAAGAATGAACCAGAATGGAAAA
  AACGTAAAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223110 representing NM_001081402
  Red=Cloning site Green=Tags(s)
  
  MEHSGTSEVTGADTAGPDPQLAVTMGFTGFGKKARTFDLEAMFEQTRRTAVERSRKTLEAREKEEEMNRE
  KELRKQIEDMEPAPSSSSAARERSQSSCRDTSSSDSESDDSSDSSDDELIGPPLPPKMVGESVTTVDEGT
  LGPLPPPLCEEGEDDDDDELDDEGEEDNPVQRIPDSHEITLRHGTKTVSALGLDPSGARLVTGGYDYDVK
  FWDFAGMDASFKAFRSLQPCECHQIKSLQYSNTGDMILVVSGSSQAKVIDRDGFEVMECIKGDQYIVDMA
  NTKGHTAMLHTGSWHPKIKGEFMTCSNDATVRLWEVENPKKQKSVFKPRTMQGKKVIPTTCTYSRDGNLV
  AAACQNGSIQIWDRNLTVHPKFHYKQAHDPGTDTSCVAFSYDGNVLASRGGDDTLKLWDVRQFNKPLFSA
  SDLPTLFPMTDCCFSPDDKLIVTGTSVQRGCGSGKLVFLERRTFQRVYEIHITDASVVRCLWHPKLNQIM
  VGTGNGLAKVYYDPNKSQRGAKLCVVKTQRKAKQAETLTQDYIITPHALPMFREPRQRSTRKQLEKDRLD
  PLKSHKPEPPVAGPGRGGRVGTHGGTLSSYIVKNIALDKTDDSNPREAILRHAKAAEDNPYWVSPAYSKT
  QPKTMFAQVESDDEESKNEPEWKKRKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081402
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081402.1, NP_001074871.1
• RefSeq Size: 2151 bp
• RefSeq ORF: 1974 bp
• Locus ID: 545085
• UniProt ID: Q3TWF6
• Cytogenetics: 15 A1
• MW: 73.5 kDa