Braf (NM_139294) Mouse Tagged ORF Clone

USD 750.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name Braf (NM_139294) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Braf
Synonyms 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; Braf-2; Braf2; C87398; C230098H17; D6Ertd631e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR223023 representing NM_139294
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCTGAGTGGCGGCGGTGGCAGCAGCAGCGGTGGCGGCGGCGGCGGTGGCGGCGGCGGTGGCG
GTGGCGACGGCGGCGGCGGCGCCGAGCAGGGCCAGGCTCTGTTCAATGGCGACATGGAGCCGGAGGCCGG
CGCTGGCGCCGCGGCCTCTTCGGCTGCGGACCCGGCCATTCCTGAAGAGGTATGGAATATCAAGCAAATG
ATTAAGTTGACACAGGAACATATAGAGGCCCTATTGGACAAATTTGGTGGAGAGCATAACCCACCATCAA
TATACCTGGAGGCCTATGAAGAGTACACCAGCAAGCTAGATGCCCTTCAGCAAAGAGAACAGCAGCTTTT
GGAATCCCTGGTTTTTCAAACTCCCACAGATGCATCACGGAACAACCCCAAGTCACCACAGAAACCTATC
GTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCCGCAAGATGTGGTGTTACAGTTCGAGACA
GTCTAAAGAAAGCACTGATGATGAGAGGTCTCATCCCAGAATGCTGTGCTGTTTACAGAATTCAGGATGG
AGAGAAGAAACCAATTGGCTGGGACACGGACATTTCCTGGCTTACTGGAGAGGAGTTACATGTTGAAGTA
CTGGAGAATGTCCCACTTACAACACACAACTTTGTACGGAAAACTTTTTTCACCTTAGCATTTTGTGACT
TTTGCCGAAAGCTGCTTTTCCAGGGTTTCCGTTGTCAAACATGTGGTTATAAATTTCACCAGCGTTGTAG
TACAGAGGTTCCACTGATGTGTGTAAATTATGACCAACTTGATTTGCTGTTTGTCTCCAAGTTCTTTGAG
CATCACCCAGTACCACAGGAGGAGGCCTCCTTCCCAGAGACTGCCCTTCCATCTGGATCCTCTTCCGCAC
CCCCCTCAGACTCTACTGGGCCCCAAATCCTCACCAGTCCATCTCCTTCAAAATCCATTCCAATTCCACA
GCCCTTCCGACCAGCAGATGAAGATCATCGCAATCAGTTTGGGCAACGAGACCGGTCCTCCTCAGCTCCC
AATGTTCATATAAACACAATTGAGCCTGTGAATATCGATGAAAAATTCCCAGAAGTGGAATTACAGGATC
AAAGGGATTTGATTAGAGACCAGGGGTTTCGTGGTGATGGAGCCCCCTTGAACCAACTGATGCGCTGTCT
TCGGAAATACCAATCCCGGACTCCCAGCCCCCTCCTCCATTCTGTCCCCAGTGAAATAGTGTTTGATTTT
GAGCCTGGCCCAGTGTTCAGAGGGTCAACCACAGGCTTGTCCGCCACCCCGCCTGCCTCATTACCTGGCT
CACTCACTAACGTGAAAGCCTTACAGAAATCTCCAGGTCCTCAGCGGGAAAGGAAGTCATCTTCTTCCTC
ATCCTCGGAGGACAGAAGTCGGATGAAAACACTTGGTAGAAGAGATTCAAGTGATGACTGGGAGATTCCT
GATGGACAGATTACAGTGGGACAGAGAATTGGATCTGGGTCATTTGGAACTGTCTACAAGGGAAAGTGGC
ATGGTGATGTGGCAGTGAAAATGTTGAATGTGACAGCACCCACACCTCAACAGCTACAGGCCTTCAAAAA
TGAAGTAGGAGTGCTCAGGAAAACTCGACATGTGAATATCCTCCTTTTCATGGGCTATTCTACAAAGCCA
CAACTGGCAATTGTTACACAGTGGTGTGAGGGCTCCAGCTTATATCACCATCTCCACATCATTGAGACCA
AATTTGAGATGATCAAACTTATAGATATTGCTCGGCAGACTGCACAGGGCATGGATTACTTACACGCCAA
GTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTTCTTCATGAAGACCTCACGGTAAAAATAGGT
GACTTTGGTCTAGCCACAGTGAAATCTCGGTGGAGTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCTA
TTTTGTGGATGGCACCAGAAGTAATCAGAATGCAAGATAAAAACCCGTATAGCTTTCAGTCAGACGTGTA
TGCGTTTGGGATTGTTCTGTACGAACTGATGACCGGCCAGCTACCTTATTCAAACATCAACAACAGGGAT
CAGATAATTTTTATGGTGGGACGAGGATACCTATCTCCAGATCTCAGTAAGGTACGGAGTAACTGTCCAA
AAGCCATGAAGAGATTAATGGCAGAGTGCCTCAAAAAGAAAAGAGACGAGAGACCACTCTTTCCCCAAAT
TCTCGCCTCCATTGAGCTGCTGGCCCGCTCATTGCCAAAAATTCACCGCAGTGCATCAGAACCTTCCTTG
AATCGGGCTGGTTTCCAAACAGAAGATTTTAGTCTGTATGCTTGTGCTTCTCCGAAAACACCCATCCAAG
CAGGGGGATATGGAGAATTTGCAGCCTTCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR223023 representing NM_139294
Red=Cloning site Green=Tags(s)

MAALSGGGGSSSGGGGGGGGGGGGGDGGGGAEQGQALFNGDMEPEAGAGAAASSAADPAIPEEVWNIKQM
IKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLVFQTPTDASRNNPKSPQKPI
VRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEV
LENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFE
HHPVPQEEASFPETALPSGSSSAPPSDSTGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAP
NVHINTIEPVNIDEKFPEVELQDQRDLIRDQGFRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDF
EPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSSSEDRSRMKTLGRRDSSDDWEIP
DGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKP
QLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIG
DFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRD
QIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSL
NRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_139294
ORF Size 2412 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_139294.3, NM_139294.4, NM_139294.5, NP_647455.3
RefSeq Size 9728 bp
RefSeq ORF 2415 bp
Locus ID 109880
Cytogenetics 6 18.43 cM
MW 89.2 kDa

Citations (1)

Other Versions

Other products for "Braf"
Frequently bought together (2)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

USD 310.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 570.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
68 Mouse Clones
Molbio tools