Braf (NM_139294) Mouse Tagged ORF Clone

CAT#: MR223023

Braf (Myc-DDK-tagged) - Mouse Braf transforming gene (Braf)

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Size
    • 10 ug

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Specifications

Product Data
Product Name Braf (NM_139294) Mouse Tagged ORF Clone
Symbol Braf
Synonyms 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; Braf-2; Braf2; C230098H17; C87398;
Vector pCMV6-Entry
Sequence Data
ORF Nucleotide Sequence
>MR223023 representing NM_139294
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCTGAGTGGCGGCGGTGGCAGCAGCAGCGGTGGCGGCGGCGGCGGTGGCGGCGGCGGTGGCG
GTGGCGACGGCGGCGGCGGCGCCGAGCAGGGCCAGGCTCTGTTCAATGGCGACATGGAGCCGGAGGCCGG
CGCTGGCGCCGCGGCCTCTTCGGCTGCGGACCCGGCCATTCCTGAAGAGGTATGGAATATCAAGCAAATG
ATTAAGTTGACACAGGAACATATAGAGGCCCTATTGGACAAATTTGGTGGAGAGCATAACCCACCATCAA
TATACCTGGAGGCCTATGAAGAGTACACCAGCAAGCTAGATGCCCTTCAGCAAAGAGAACAGCAGCTTTT
GGAATCCCTGGTTTTTCAAACTCCCACAGATGCATCACGGAACAACCCCAAGTCACCACAGAAACCTATC
GTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCCGCAAGATGTGGTGTTACAGTTCGAGACA
GTCTAAAGAAAGCACTGATGATGAGAGGTCTCATCCCAGAATGCTGTGCTGTTTACAGAATTCAGGATGG
AGAGAAGAAACCAATTGGCTGGGACACGGACATTTCCTGGCTTACTGGAGAGGAGTTACATGTTGAAGTA
CTGGAGAATGTCCCACTTACAACACACAACTTTGTACGGAAAACTTTTTTCACCTTAGCATTTTGTGACT
TTTGCCGAAAGCTGCTTTTCCAGGGTTTCCGTTGTCAAACATGTGGTTATAAATTTCACCAGCGTTGTAG
TACAGAGGTTCCACTGATGTGTGTAAATTATGACCAACTTGATTTGCTGTTTGTCTCCAAGTTCTTTGAG
CATCACCCAGTACCACAGGAGGAGGCCTCCTTCCCAGAGACTGCCCTTCCATCTGGATCCTCTTCCGCAC
CCCCCTCAGACTCTACTGGGCCCCAAATCCTCACCAGTCCATCTCCTTCAAAATCCATTCCAATTCCACA
GCCCTTCCGACCAGCAGATGAAGATCATCGCAATCAGTTTGGGCAACGAGACCGGTCCTCCTCAGCTCCC
AATGTTCATATAAACACAATTGAGCCTGTGAATATCGATGAAAAATTCCCAGAAGTGGAATTACAGGATC
AAAGGGATTTGATTAGAGACCAGGGGTTTCGTGGTGATGGAGCCCCCTTGAACCAACTGATGCGCTGTCT
TCGGAAATACCAATCCCGGACTCCCAGCCCCCTCCTCCATTCTGTCCCCAGTGAAATAGTGTTTGATTTT
GAGCCTGGCCCAGTGTTCAGAGGGTCAACCACAGGCTTGTCCGCCACCCCGCCTGCCTCATTACCTGGCT
CACTCACTAACGTGAAAGCCTTACAGAAATCTCCAGGTCCTCAGCGGGAAAGGAAGTCATCTTCTTCCTC
ATCCTCGGAGGACAGAAGTCGGATGAAAACACTTGGTAGAAGAGATTCAAGTGATGACTGGGAGATTCCT
GATGGACAGATTACAGTGGGACAGAGAATTGGATCTGGGTCATTTGGAACTGTCTACAAGGGAAAGTGGC
ATGGTGATGTGGCAGTGAAAATGTTGAATGTGACAGCACCCACACCTCAACAGCTACAGGCCTTCAAAAA
TGAAGTAGGAGTGCTCAGGAAAACTCGACATGTGAATATCCTCCTTTTCATGGGCTATTCTACAAAGCCA
CAACTGGCAATTGTTACACAGTGGTGTGAGGGCTCCAGCTTATATCACCATCTCCACATCATTGAGACCA
AATTTGAGATGATCAAACTTATAGATATTGCTCGGCAGACTGCACAGGGCATGGATTACTTACACGCCAA
GTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTTCTTCATGAAGACCTCACGGTAAAAATAGGT
GACTTTGGTCTAGCCACAGTGAAATCTCGGTGGAGTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCTA
TTTTGTGGATGGCACCAGAAGTAATCAGAATGCAAGATAAAAACCCGTATAGCTTTCAGTCAGACGTGTA
TGCGTTTGGGATTGTTCTGTACGAACTGATGACCGGCCAGCTACCTTATTCAAACATCAACAACAGGGAT
CAGATAATTTTTATGGTGGGACGAGGATACCTATCTCCAGATCTCAGTAAGGTACGGAGTAACTGTCCAA
AAGCCATGAAGAGATTAATGGCAGAGTGCCTCAAAAAGAAAAGAGACGAGAGACCACTCTTTCCCCAAAT
TCTCGCCTCCATTGAGCTGCTGGCCCGCTCATTGCCAAAAATTCACCGCAGTGCATCAGAACCTTCCTTG
AATCGGGCTGGTTTCCAAACAGAAGATTTTAGTCTGTATGCTTGTGCTTCTCCGAAAACACCCATCCAAG
CAGGGGGATATGGAGAATTTGCAGCCTTCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR223023 representing NM_139294
Red=Cloning site Green=Tags(s)

MAALSGGGGSSSGGGGGGGGGGGGGDGGGGAEQGQALFNGDMEPEAGAGAAASSAADPAIPEEVWNIKQM
IKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLVFQTPTDASRNNPKSPQKPI
VRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEV
LENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFE
HHPVPQEEASFPETALPSGSSSAPPSDSTGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAP
NVHINTIEPVNIDEKFPEVELQDQRDLIRDQGFRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDF
EPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSSSEDRSRMKTLGRRDSSDDWEIP
DGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKP
QLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIG
DFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRD
QIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSL
NRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_139294
ORF Size 2412 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_139294.3, NM_139294.4, NM_139294.5, NP_647455
RefSeq Size 9728
RefSeq ORF 2415
Locus ID 109880
Cytogenetics 6 B1|6 18.43 cM
MW 89.2 kDa
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Citations