Mrgpra1 (NM_153095) Mouse Tagged ORF Clone

CAT#: MR222931

Mrgpra1 (Myc-DDK-tagged) - Mouse MAS-related GPR, member A1 (Mrgpra1)

Product Images

Circular map for MR222931

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mrgpra1
• Synonyms: MrgA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222931 representing NM_153095
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAAAGCAGCACCTGTGCAGGGTTTCTAGCCCTAAACACATCGGCCTCGCCAACAGCACCCACAA
  CAACTAATCCAATGGACAATACCATCCCTGGAGGTATCAACATCACGATTCTGATCCCAAACTTGATGAT
  CATCATCTTCGGACTGGTCGGGCTGACAGGAAATGGCATTGTGTTCTGGCTCCTGGGCTTCTGTTTGCAC
  AGGAACGCCTTCTCAGTCTACATCCTAAACTTAGCTCTAGCTGACTTCTTCTTCCTCCTAGGTCACATCA
  TAGATTCCATACTGCTTCTTCTCAATGTTTTCTACCCAATTACCTTTCTCTTGTGCTTTTACACGATCAT
  GATGGTTCTCTATATCGCAGGCCTGAGCATGCTCAGTGCCATCAGCACTGAGCGCTGCCTGTCTGTACTG
  TGCCCCATCTGGTATCACTGTCACCGCCCAGAACACACATCAACTGTCATGTGTGCTGTCATCTGGGTCC
  TGTCCCTGTTGATCTGCATTCTGAATAGTTATTTCTGCGGTTTCTTAAATACCCAATATAAAAATGAAAA
  TGGGTGTCTGGCATTGAGCTTCTTTACTGCTGCATACCTGATGTTTTTGTTTGTGGTCCTCTGTCTGTCC
  AGCCTGGCTCTGGTGGCCAGGTTGTTCTGTGGTACTGGGCAGATAAAGCTTACCAGATTGTATGTAACCA
  TTATTCTGAGCATTTTGGTTTTTCTCCTTTGCGGATTGCCCTTTGGCATCCACTGGTTTCTGTTATTCAA
  GATTAAGGATGATTTTCATGTATTTGATCTTGGATTTTATCTGGCATCAGTTGTCCTGACTGCTATTAAT
  AGCTGTGCCAACCCCATCATTTACTTCTTCGTGGGATCCTTCAGGCATCGGTTGAAGCACCAGACCCTCA
  AAATGGTTCTCCAGAATGCACTGCAAGACACTCCTGAGACAGCCAAAATCATGGTGGAGATGTCAAGAAG
  CAAATCAGAGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222931 representing NM_153095
  Red=Cloning site Green=Tags(s)
  
  MGESSTCAGFLALNTSASPTAPTTTNPMDNTIPGGINITILIPNLMIIIFGLVGLTGNGIVFWLLGFCLH
  RNAFSVYILNLALADFFFLLGHIIDSILLLLNVFYPITFLLCFYTIMMVLYIAGLSMLSAISTERCLSVL
  CPIWYHCHRPEHTSTVMCAVIWVLSLLICILNSYFCGFLNTQYKNENGCLALSFFTAAYLMFLFVVLCLS
  SLALVARLFCGTGQIKLTRLYVTIILSILVFLLCGLPFGIHWFLLFKIKDDFHVFDLGFYLASVVLTAIN
  SCANPIIYFFVGSFRHRLKHQTLKMVLQNALQDTPETAKIMVEMSRSKSEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153095
• ORF Size: 993 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153095.2, NP_694735.2
• RefSeq Size: 1088 bp
• RefSeq ORF: 996 bp
• Locus ID: 233221
• UniProt ID: Q91WW5
• Cytogenetics: 7 B3
• MW: 37 kDa
• Gene Summary: Orphan receptor activated by a subset of RFamide-family neuropeptides such as FLRF-amide and FMRF-amide. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. May regulate the function of nociceptive neurons by modulation of pain perception.[UniProtKB/Swiss-Prot Function]