Tfpi (NM_001177319) Mouse Tagged ORF Clone

CAT#: MR222864

Tfpi (Myc-DDK-tagged) - Mouse tissue factor pathway inhibitor (Tfpi), transcript variant 2

Product Images

Circular map for MR222864

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tfpi
• Synonyms: A630013F22Rik; AW552122; EPI; LACI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222864 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTACAAAATGAAGAAAGAACATGCCTTTTGGGCCACTGTGTGTCTGTTGCTTAGCCTTGTTCCCG
  AGTTTCTTAATGCTCTGTCTGAGGAAGCTGATGACACAGATTCTGAGCTGGGGTCAATGAAACCGCTACA
  TACATTTTGTGCAATGAAGGCAGATGATGGTCCATGCAAAGCAATGATAAGGAGTTATTTTTTCAATATG
  TATACTCATCAATGTGAAGAATTTATATACGGGGGATGTGAAGGGAACGAGAACCGATTTGATACCCTGG
  AAGAGTGTAAGAAAACATGCATACCAGGTTATGAGAAGACAGCTGTGAAGGCAGCATCTGGAGCAGAAAG
  GCCAGATTTCTGCTTCTTGGAAGAGGACCCGGGACTCTGCCGAGGTTACATGAAGAGGTATCTTTATAAC
  AACCAGACAAAGCAGTGTGAACGATTCGTGTACGGTGGCTGCCTGGGCAACCGCAACAACTTTGAAACTT
  TGGATGAGTGCAAGAAGATCTGTGAGAATCCAGTCCACTCCCCTTCCCCAGTGAATGAGGTACAGATGAG
  TGACTACGTAACTGATGGAAATACTGTAACTGATCGCAGTACTGTAAATAACATCGTGGTTCCCCAGTCT
  CCCAAAGTGCCCAGGCGTCGGGCTGCCTTGGTACTGTATCACCTTTATGATATTTTGAAAAAACTCATCT
  TTGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222864 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTYKMKKEHAFWATVCLLLSLVPEFLNALSEEADDTDSELGSMKPLHTFCAMKADDGPCKAMIRSYFFNM
  YTHQCEEFIYGGCEGNENRFDTLEECKKTCIPGYEKTAVKAASGAERPDFCFLEEDPGLCRGYMKRYLYN
  NQTKQCERFVYGGCLGNRNNFETLDECKKICENPVHSPSPVNEVQMSDYVTDGNTVTDRSTVNNIVVPQS
  PKVPRRRAALVLYHLYDILKKLIFG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177319
• ORF Size: 708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177319.1, NP_001170790.1
• RefSeq Size: 3027 bp
• RefSeq ORF: 708 bp
• Locus ID: 21788
• Cytogenetics: 2 D
• MW: 26.8 kDa
• Gene Summary: Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma (By similarity).[UniProtKB/Swiss-Prot Function]