Mpp5 (NM_019579) Mouse Tagged ORF Clone

CAT#: MR222682

Mpp5 (Myc-DDK-tagged) - Mouse membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (Mpp5)

Product Images

Circular map for MR222682

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mpp5
• Synonyms: 3830420B02Rik; AI255216; AI644496; Pals1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222682 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACATCATATATGAATGGGCATGTAACAGAGGAATCAGACAGCGGAATAAAAAATCTTGATCTTG
  CATCACCAGAGGAATATCCGAAGCACCGAGAGATGGCTGTTGACTGCCCTGGAGATTTGGGCACCAGAAT
  GATGCCAGTGCGAAGAAGTGCACAGTTAGAGCGGATTCGACAACAACAGGAGGATATGAGACGTAGGCGC
  GAAGAGGAAGGGAAAAAGCAAGAACTTGACCTAAATTCTTCCATGAGACTTAAGAAACTAGCTCAAATTC
  CTCCAAAGACTGGAATAGATAACCCTATATTTGACACGGAAGAAGGAATTGTTTTAGAAAGTCCTCACTA
  TGCTGTGAACATATTAGACGTTGAAGACTTGTTCTCTTCACTTAAACACATCCAACACACTTTGGTTGAT
  TCTCAGAGCCAGGAGGATATCTCACTGCTTTTACAGCTTGTACAAAACAGAGATTTTCAGAATGCATTCA
  AGATACACAATGCTGTCACAGTACACATGAGCAAGGCCAGCCCTCCGTTTCCTCTTATCGCCAATGTACA
  AGACCTTGTACAAGAGGTACAAACTGTCTTAAAGCCAGTCCATCAGAAGGAAGGACAAGAACTAACTGCC
  TTGTTGAATGCTCCACATATTCAGGCACTTTTACTGGCCCATGATAAGGTTGCTGAGCAGGAAATGCAGC
  TAGAGCCCATTACAGATGAGAGAGTTTATGAAAGTATCGGCCATTACGGGGGAGAAACTGTAAAAATAGT
  TCGTATAGAAAAGGCTCGGGATATTCCATTGGGTGCCACAGTTCGTAATGAAATGGATTCTGTCATCATA
  AGCCGGATAGTGAAAGGAGGCGCCGCAGAGAAGAGTGGCCTCCTGCACGAAGGAGACGAAGTTCTGGAGA
  TCAACGGCATTGAAATCCGGGGCAAGGATGTCAATGAGGTTTTTGACTTGTTGTCTGATATGCATGGTAC
  TTTGACATTTGTTCTGATTCCTAGTCAACAGATCAAGCCCCCTCCTGCCAAAGAAACTGTAATCCATGTG
  AAAGCTCATTTTGACTATGACCCCTCAGATGACCCTTATGTTCCATGTCGAGAGTTAGGGCTGTCTTTTC
  AAAAAGGAGATATACTTCATGTGATCAGTCAAGAAGATCCAAATTGGTGGCAGGCCTATAGAGAGGGAGA
  CGAAGATAATCAGCCTCTAGCTGGACTTGTTCCAGGCAAAAGCTTTCAGCAGCAAAGGGAAGCCATGAAG
  CAAACTATAGAAGAAGATAAGGAGCCAGAGAAATCAGGAAAACTGTGGTGTGCAAAGAAGAATAAAAAGA
  AGAGGAAAAAGGTTTTATATAATGCCAATAAAAATGATGATTATGACAATGAAGAGATCTTAACGTATGA
  GGAAATGTCACTTTATCACCAGCCAGCAAATAGGAAAAGACCTATCATCTTGATCGGTCCACAGAACTGT
  GGCCAGAATGAACTGCGCCAGAGACTAATGAATAAGGAAAAAGACCGCTTTGCATCTGCCGTTCCTCATA
  CAACTCGGAATAGGCGAGACCATGAAGTAGCTGGGAGAGATTACCACTTTGTTTCTCGGCAAGCATTTGA
  AGCAGACATAGCAGCTGGCAAGTTCATTGAACATGGTGAATTTGAGAAAAATCTGTATGGAACCAGCATA
  GACTCTGTACGACAAGTAATCAACTCTGGCAAGATATGTCTCTTAAGTCTTCGCACACAGTCCTTGAAGA
  CCCTCCGGAATTCAGATTTGAAACCATATATCATCTTCATTGCACCACCTTCACAAGAGAGACTTCGGGC
  GTTGTTAGCCAAGGAAGGCAAGAACCCAAAGCCTGAAGAATTAAGGGAGATCATTGAGAAGACGAGAGAG
  ATGGAGCAGAACAACGGACACTACTTCGACACTGCAATTGTGAATTCAGATCTTGATAAAGCCTATCAGG
  AATTGCTTAGGCTCATTAACAAACTGGATACTGAACCTCAGTGGGTGCCATCCACCTGGCTAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222682 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTTSYMNGHVTEESDSGIKNLDLASPEEYPKHREMAVDCPGDLGTRMMPVRRSAQLERIRQQQEDMRRRR
  EEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPIFDTEEGIVLESPHYAVNILDVEDLFSSLKHIQHTLVD
  SQSQEDISLLLQLVQNRDFQNAFKIHNAVTVHMSKASPPFPLIANVQDLVQEVQTVLKPVHQKEGQELTA
  LLNAPHIQALLLAHDKVAEQEMQLEPITDERVYESIGHYGGETVKIVRIEKARDIPLGATVRNEMDSVII
  SRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPPPAKETVIHV
  KAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMK
  QTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNC
  GQNELRQRLMNKEKDRFASAVPHTTRNRRDHEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSI
  DSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPPSQERLRALLAKEGKNPKPEELREIIEKTRE
  MEQNNGHYFDTAIVNSDLDKAYQELLRLINKLDTEPQWVPSTWLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019579
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019579.3, NP_062525.1
• RefSeq Size: 5529 bp
• RefSeq ORF: 2028 bp
• Locus ID: 56217
• UniProt ID: Q9JLB2
• Cytogenetics: 12 C3
• MW: 77.2 kDa
• Gene Summary: Plays a role in tight junction biogenesis and in the establishment of cell polarity in epithelial cells (By similarity). Also involved in adherens junction biogenesis by ensuring correct localization of the exocyst complex protein EXOC4/SEC8 which allows trafficking of adherens junction structural component CDH1 to the cell surface (PubMed:17182851, PubMed:20237282). Plays a role through its interaction with CDH5 in vascular lumen formation and endothelial membrane polarity (By similarity). Required during embryonic and postnatal retinal development (PubMed:22398208). Required for the maintenance of cerebellar progenitor cells in an undifferentiated proliferative state, preventing premature differentiation, and is required for cerebellar histogenesis, fissure formation and cerebellar layer organization (PubMed:26657772). Plays a role in the radial and longitudinal extension of the myelin sheath in Schwann cells (PubMed:20237282). May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter (PubMed:15234345). May play a role in the T-cell receptor-mediated activation of NF-kappa-B (By similarity). Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton (PubMed:15677456). Required for the normal polarized localization of the vesicular marker STX4 (PubMed:20237282). Required for the correct trafficking of the myelin proteins PMP22 and MAG (By similarity).[UniProtKB/Swiss-Prot Function]