Axl (NM_001190974) Mouse Tagged ORF Clone

CAT#: MR222556

Axl (Myc-DDK-tagged) - Mouse AXL receptor tyrosine kinase (Axl), transcript variant 2

Product Images

Circular map for MR222556

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Axl
• Synonyms: AI323647; Ark; Tyro7; Ufo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222556 representing NM_001190974
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGGGTCCCGCTGGCCTGGTGGTTGGCGCTGTGCTGCTGGGGGTGTGCAGCCCATAAGGACACAC
  AGACCGAGGCTGGCAGCCCGTTTGTGGGGAACCCAGGGAATATCACAGGTGCCAGAGGACTCACGGGGAC
  ACTTCGGTGTGAGCTCCAGGTTCAGGGGGAACCCCCTGAGGTGGTGTGGCTTCGAGATGGACAGATCCTA
  GAACTGGCTGATAACACCCAGACCCAGGTGCCTCTGGGCGAAGACTGGCAAGATGAATGGAAAGTTGTCA
  GTCAGCTCAGAATCTCAGCCCTGCAACTTTCAGATGCAGGGGAGTACCAGTGTATGGTGCATCTAGAAGG
  ACGGACCTTTGTGTCTCAGCCGGGCTTTGTAGGGCTGGAAGGTCTCCCGTACTTCCTGGAGGAGCCTGAG
  GACAAAGCTGTGCCTGCCAACACCCCTTTCAACCTAAGCTGCCAGGCCCAGGGACCCCCGGAACCCGTGA
  CCCTACTCTGGCTTCAAGATGCTGTCCCCCTGGCCCCAGTCACAGGACACAGCTCCCAGCACAGTCTGCA
  AACTCCAGGCCTGAACAAGACATCTTCTTTCTCATGTGAAGCCCACAATGCCAAGGGAGTCACCACCTCC
  CGCACAGCCACCATCACAGTGCTCCCCCAGAGGCCTCACCATCTCCACGTGGTTTCCAGACAACCTACGG
  AGCTAGAGGTAGCTTGGACCCCTGGCCTGAGTGGCATCTACCCGCTCACCCACTGCAACCTGCAGGCCGT
  GCTGTCAGACGATGGGGTGGGTATCTGGCTGGGAAAGTCAGATCCTCCTGAAGACCCCCTCACCTTGCAA
  GTATCAGTGCCCCCCCACCAGCTTCGGCTGGAAAAGCTCCTTCCTCACACCCCGTATCACATCCGGATAT
  CCTGCAGCAGCAGCCAGGGCCCCTCACCTTGGACCCACTGGCTTCCTGTGGAGACCACAGAGGGAGTGCC
  CTTGGGTCCCCCTGAGAACGTTAGCGCCATGCGGAATGGGAGCCAGGTCCTCGTGCGTTGGCAGGAGCCA
  AGGGTGCCCCTGCAAGGCACCCTGTTAGGGTACCGGCTGGCATATCGAGGCCAGGACACCCCCGAGGTAC
  TTATGGATATAGGGCTAACTCGAGAGGTGACCTTGGAACTGCGGGGGGACAGGCCTGTGGCTAACCTGAC
  TGTGTCTGTGACAGCCTATACCTCGGCTGGGGATGGGCCCTGGAGCCTTCCTGTGCCCCTAGAGCCCTGG
  CGCCCAGTGAGTGAACCCCCACCTCGCGCCTTCTCGTGGCCTTGGTGGTATGTACTGCTGGGAGCACTTG
  TGGCTGCCGCCTGCGTCCTCATCTTGGCCCTGTTCCTTGTCCATCGGAGGAAGAAGGAGACTCGATATGG
  GGAGGTGTTTGAGCCAACCGTGGAAAGAGGTGAACTGGTAGTCAGGTACCGTGTCCGAAAGTCCTACAGC
  CGGCGGACCACTGAAGCCACCTTGAACAGTCTGGGCATCAGTGAAGAGCTGAAGGAGAAACTACGAGACG
  TCATGGTAGATCGGCATAAGGTGGCCTTGGGGAAGACCCTGGGAGAAGGAGAATTTGGCGCTGTGATGGA
  AGGTCAGCTCAATCAGGATGACTCCATCCTCAAGGTCGCTGTGAAGACCATGAAAATTGCCATCTGCACA
  AGATCAGAGCTGGAGGATTTCCTGAGTGAAGCTGTCTGCATGAAGGAATTTGACCACCCCAACGTCATGA
  GGCTCATTGGCGTCTGTTTTCAGGGCTCTGACAGAGAGGGTTTCCCAGAACCTGTGGTCATCTTGCCTTT
  CATGAAACACGGAGACCTACACAGTTTCCTCCTGTACTCCCGGCTCGGGGACCAGCCAGTGTTCCTGCCC
  ACTCAGATGCTAGTGAAGTTCATGGCCGACATTGCCAGTGGTATGGAGTACCTGAGTACCAAGAGATTCA
  TACATCGGGACCTGGCTGCCAGGAACTGCATGCTGAATGAGAACATGTCCGTGTGTGTGGCAGACTTCGG
  GCTCTCCAAGAAGATCTACAACGGGGATTACTACCGCCAAGGGCGCATTGCCAAGATGCCAGTCAAGTGG
  ATTGCTATTGAGAGTCTGGCAGATCGGGTCTACACCAGCAAGAGCGATGTGTGGTCCTTCGGTGTGACAA
  TGTGGGAGATCGCCACCCGAGGCCAAACTCCCTATCCAGGGGTGGAGAACAGTGAGATTTACGACTACCT
  GCGTCAAGGAAATCGGCTGAAACAGCCTGTGGACTGTCTGGACGGCCTGTATGCCCTGATGTCTCGGTGC
  TGGGAACTGAACCCTCGAGACCGGCCAAGTTTTGCGGAGCTCCGGGAAGACTTGGAGAACACACTGAAGG
  CTCTGCCCCCTGCTCAGGAGCCAGATGAAATCCTCTATGTCAACATGGATGAGGGCGGAAGCCACCTTGA
  ACCCCGTGGGGCTGCTGGAGGAGCTGACCCCCCAACCCAACCTGATCCTAAGGATTCCTGTAGCTGTCTC
  ACTGCAGCTGACGTCCACTCAGCTGGACGCTATGTCCTTTGTCCTTCTACAGCCCCAGGACCCACTCTGT
  CTGCTGACAGAGGCTGCCCAGCACCTCCAGGGCAGGAGGACGGAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222556 representing NM_001190974
  Red=Cloning site Green=Tags(s)
  
  MGRVPLAWWLALCCWGCAAHKDTQTEAGSPFVGNPGNITGARGLTGTLRCELQVQGEPPEVVWLRDGQIL
  ELADNTQTQVPLGEDWQDEWKVVSQLRISALQLSDAGEYQCMVHLEGRTFVSQPGFVGLEGLPYFLEEPE
  DKAVPANTPFNLSCQAQGPPEPVTLLWLQDAVPLAPVTGHSSQHSLQTPGLNKTSSFSCEAHNAKGVTTS
  RTATITVLPQRPHHLHVVSRQPTELEVAWTPGLSGIYPLTHCNLQAVLSDDGVGIWLGKSDPPEDPLTLQ
  VSVPPHQLRLEKLLPHTPYHIRISCSSSQGPSPWTHWLPVETTEGVPLGPPENVSAMRNGSQVLVRWQEP
  RVPLQGTLLGYRLAYRGQDTPEVLMDIGLTREVTLELRGDRPVANLTVSVTAYTSAGDGPWSLPVPLEPW
  RPVSEPPPRAFSWPWWYVLLGALVAAACVLILALFLVHRRKKETRYGEVFEPTVERGELVVRYRVRKSYS
  RRTTEATLNSLGISEELKEKLRDVMVDRHKVALGKTLGEGEFGAVMEGQLNQDDSILKVAVKTMKIAICT
  RSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSDREGFPEPVVILPFMKHGDLHSFLLYSRLGDQPVFLP
  TQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKW
  IAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRQGNRLKQPVDCLDGLYALMSRC
  WELNPRDRPSFAELREDLENTLKALPPAQEPDEILYVNMDEGGSHLEPRGAAGGADPPTQPDPKDSCSCL
  TAADVHSAGRYVLCPSTAPGPTLSADRGCPAPPGQEDGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190974
• ORF Size: 2637 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190974.1, NP_001177903.1
• RefSeq Size: 5057 bp
• RefSeq ORF: 2640 bp
• Locus ID: 26362
• Cytogenetics: 7 14.02 cM
• MW: 97.7 kDa
• Gene Summary: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.[UniProtKB/Swiss-Prot Function]