Tie1 (NM_011587) Mouse Tagged ORF Clone

CAT#: MR222502

Tie1 (Myc-DDK-tagged) - Mouse tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1)

Product Images

Circular map for MR222502

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tie1
• Synonyms: D430008P04Rik; TIE; tie-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222502 representing NM_011587
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGGTGGGGATCCTCTTTGCTGCTCCCCACTCTTTTCTTGGCCTCTCATGTTGGTGCGTCTGTTG
  ACTTAACATTGCTGGCCAACCTGCGCATCACAGACCCCCAGCGTTTCTTCCTGACCTGCGTGTCTGGTGA
  GGCCGGAGCAGGGAGGAGCTCGGACCCGCCCCTGCTGCTGGAGAAGGATGATCGCATCGTGCGCACCTTC
  CCGCCTGGGCAGCCCCTGTACCTGGCACGCAACGGTTCTCACCAGGTCACGCTGAGGGGCTTCTCCAAGC
  CCTCGGATTTGGTAGGCGTCTTCTCCTGTGTGGGTGGTGCTGGCGCGCGGCGCACTCGAGTTCTCTATGT
  GCACAACAGCCCAGGGGCACACCTGTTTCCAGACAAGGTCACACACACGGTGAACAAAGGTGACACTGCT
  GTGCTTTCTGCCCATGTGCACAAGGAAAAGCAGACTGACGTGATCTGGAAGAACAACGGATCCTACTTCA
  ATACCCTAGACTGGCAAGAGGCCGATGACGGGCGTTTTCAACTGCAGCTCCAAAATGTACAGCCACCATC
  AAGTGGCATCTACAGTGCCACCTACCTAGAGGCCAGCCCCCTGGGCAGTGCCTTCTTTCGGCTCATCGTG
  CGAGGCTGTGGGGCTGGACGCTGGGGGCCAGGATGTGTCAAGGATTGCCCAGGCTGCCTGCATGGAGGTG
  TCTGTCATGACCATGATGGCGAATGTGTGTGTCCCCCAGGATTCACTGGCACCCGCTGTGAGCAGGCCTG
  CAGGGAGGGTCGTTTTGGACAGAGCTGTCAGGAACAGTGCCCAGGCACAGCAGGTTGTAGAGGTCTCACC
  TTCTGCCTCCCGGATCCCTATGGCTGTTCCTGTGGATCTGGCTGGAGAGGAAGCCAGTGCCAGGAAGCAT
  GTGCACCTGGTCATTTTGGGGCTGATTGTCGCCTCCAGTGCCAGTGTCAAAATGGTGGCACTTGTGACCG
  GTTCAGCGGCTGCGTCTGCCCCTCTGGGTGGCATGGAGTCCACTGTGAAAAGTCAGACCGGATCCCCCAG
  ATCCTCAGTATGGCCACAGAGGTGGAGTTCAACATAGGGACGATGCCCCGGATCAACTGTGCAGCTGCCG
  GGAATCCCTTCCCAGTGCGGGGCAGCATGAAACTTCGCAAGCCAGATGGCACCATGCTTTTGTCTACCAA
  AGTCATTGTGGAGCCAGACAGGACCACAGCAGAGTTTGAAGTGCCCAGTTTGACTCTTGGGGACAGTGGG
  TTCTGGGAATGCCGCGTATCGACTTCTGGTGGCCAAGACAGCCGGCGCTTCAAGGTCAATGTCAAAGTAC
  CCCCAGTGCCTTTGACTGCACCTCGACTCCTGGCCAAGCAGAGCCGTCAGCTTGTGGTCTCCCCACTGGT
  CTCCTTTAGTGGGGATGGACCCATCTCCTCTGTCCGCCTGCACTACCGGCCCCAGGACAGCACGATTGCC
  TGGTCTGCCATTGTGGTGGATCCCAGTGAGAATGTGACATTAATGAACCTGAAGCCAAAGACAGGATACA
  ATGTCCGAGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAAGGAGGCTGGGGACCTTCTGCCCTTATGAC
  TACTGACTGTCCTGAGCCTTTGCTGCAGCCCTGGCTAGAGAGCTGGCACGTGGAGGGTCCTGACAGGCTC
  CGTGTGAGCTGGTCTCTACCCTCGGTGCCACTGTCCGGTGATGGTTTCCTGCTGCGCCTGTGGGATGGGG
  CCCGGGGACAGGAGAGGCGGGAGAACATCTCATTCCCCCAGGCCCGCACTGCCCTCCTGACTGGACTCAC
  GCCGGGCACCCACTACCAGCTGGATGTGCGACTGTATCACTGTACCCTCCTGGGCCCTGCCTCACCCCCT
  GCGCATGTGCACCTGCCCCCCAGCGGGCCTCCAGCTCCCCGCCACCTCCATGCCCAGGCCCTCTCAGACT
  CTGAGATTCAACTCATGTGGCAGCATCCCGAGGCTCCGTCTGGGCCTATATCCAAGTACATCGTGGAGAT
  TCAGGTGGCCGGGGGCTCAGGAGACCCACAGTGGATGGATGTAGACAGGCCCGAGGAGACAAGCATCATT
  GTTCGTGGCCTCAATGCTAGCACACGATATCTTTTCCGTGTGCGGGCCAGTGTGCAGGGTCTTGGCGACT
  GGAGCAATACAGTAGAAGAGGCCACTCTGGGCAATGGGCTGCAGAGTGAGGGCCCAGTCCGAGAAAGCCG
  GGCAGCTGAAGAAGGCCTGGATCAGCAGCTGGTCCTGGCTGTGGTAGGTTCCGTCTCTGCCACCTGCCTC
  ACCATCCTGGCTGCCCTTTTAGCCTTGGTGTGTATCCGAAGAAGCTGCCTACATCGGAGACGCACCTTCA
  CTTACCAGTCAGGATCGGGTGAAGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGACCTTGACCCGGCG
  ACCGAAGCCACAGCCTGAGCCCTTGAGTTACCCCGTGCTGGAGTGGGAGGACATCACCTTTGAGGACCTC
  ATAGGGGAGGGCAACTTTGGCCAAGTGATCCGGGCCATGATCAAGAAGGACGGGCTCAAGATGAACGCAG
  CCATCAAGATGCTAAAAGAGTATGCGTCTGAAAATGACCATCGAGACTTTGCAGGTGAACTAGAAGTTCT
  GTGCAAACTAGGACACCACCCCAATATTATCAACCTCTTGGGGGCCTGTGAGAACCGAGGCTACTTGTAT
  ATTGCTATCGAATACGCCCCCTATGGAAACCTACTGGACTTCCTGAGAAAGAGCAGGGTCCTGGAGACTG
  ATCCAGCTTTTGCTCGAGAGCATGGGACGGCCTCCACACTCAGCTCTCGGCAGCTGCTGCGCTTTGCCAG
  TGATGCAGCCAACGGCATGCAGTACCTTAGTGAGAAGCAGTTCATTCACAGGGACCTGGCTGCCCGAAAT
  GTGCTGGTCGGAGAGAACCTGGCCTCCAAGATTGCAGACTTTGGCCTTTCTCGAGGGGAGGAGGTGTATG
  TGAAGAAGACAATGGGCCGTCTCCCTGTGCGTTGGATGGCCATTGAGTCCCTCAACTACAGCGTCTATAC
  CACCAAGAGTGATGTCTGGTCCTTTGGGGTCCTCCTCTGGGAGATAGTGAGCCTTGGAGGCACGCCCTAC
  TGTGGCATGACCTGTGCTGAGCTCTACGAGAAGCTGCCTCAAGGCTATCGCATGGAGCAGCCTCGAAACT
  GCGATGACGAAGTGTACGAGCTGATGCGGCAGTGCTGGCGGGATCGGCCCTATGAGCGCCCCCCCTTTGC
  TCAGATCGCACTACAGTTGGGCCGCATGCTGGAAGCCAGGAAGGCCTACGTGAACATGTCGCTGTTTGAG
  AACTTCACCTATGCGGGCATCGATGCCACAGCTGAGGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222502 representing NM_011587
  Red=Cloning site Green=Tags(s)
  
  MVWWGSSLLLPTLFLASHVGASVDLTLLANLRITDPQRFFLTCVSGEAGAGRSSDPPLLLEKDDRIVRTF
  PPGQPLYLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVLYVHNSPGAHLFPDKVTHTVNKGDTA
  VLSAHVHKEKQTDVIWKNNGSYFNTLDWQEADDGRFQLQLQNVQPPSSGIYSATYLEASPLGSAFFRLIV
  RGCGAGRWGPGCVKDCPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGTAGCRGLT
  FCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRIPQ
  ILSMATEVEFNIGTMPRINCAAAGNPFPVRGSMKLRKPDGTMLLSTKVIVEPDRTTAEFEVPSLTLGDSG
  FWECRVSTSGGQDSRRFKVNVKVPPVPLTAPRLLAKQSRQLVVSPLVSFSGDGPISSVRLHYRPQDSTIA
  WSAIVVDPSENVTLMNLKPKTGYNVRVQLSRPGEGGEGGWGPSALMTTDCPEPLLQPWLESWHVEGPDRL
  RVSWSLPSVPLSGDGFLLRLWDGARGQERRENISFPQARTALLTGLTPGTHYQLDVRLYHCTLLGPASPP
  AHVHLPPSGPPAPRHLHAQALSDSEIQLMWQHPEAPSGPISKYIVEIQVAGGSGDPQWMDVDRPEETSII
  VRGLNASTRYLFRVRASVQGLGDWSNTVEEATLGNGLQSEGPVRESRAAEEGLDQQLVLAVVGSVSATCL
  TILAALLALVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKPQPEPLSYPVLEWEDITFEDL
  IGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACENRGYLY
  IAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARN
  VLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPY
  CGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFE
  NFTYAGIDATAEEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011587
• ORF Size: 3402 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011587.2, NP_035717.2
• RefSeq Size: 3883 bp
• RefSeq ORF: 3405 bp
• Locus ID: 21846
• UniProt ID: Q06806
• Cytogenetics: 4 54.67 cM
• MW: 125 kDa
• Gene Summary: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.[UniProtKB/Swiss-Prot Function]