Kirrel3 (NM_001190914) Mouse Tagged ORF Clone

CAT#: MR222381

Kirrel3 (Myc-DDK-tagged) - Mouse kin of IRRE like 3 (Drosophila) (Kirrel3), transcript variant E

Product Images

Circular map for MR222381

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kirrel3
• Synonyms: 1500010O20Rik; 2900036G11Rik; mKIAA1867; NEPH2; SST4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222381 representing NM_001190914
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGGACAAGTTTCGGAGAATGAATGAAGGTCAAGTCTACTCCTTCAGCCAGCAACCCCAGGACC
  AAGTGGTGGTGTCAGGACAGCCAGTGACTCTGCTGTGTGCCATCCCTGAATATGATGGCTTCGTCCTGTG
  GATCAAAGATGGCTTGGCTCTGGGTGTAGGCAGAGACCTCTCAAGTTACCCCCAGTACCTGGTGGTGGGG
  AACCACCTCTCAGGAGAGCATCACCTGAAGATCCTGAGGGCTGAGCTTCAGGATGATGCCGTGTATGAGT
  GCCAGGCCATCCAGGCTGCCATCCGGTCCCGCCCTGCACGCCTCACCGTCCTGGTGCCACCAGATGACCC
  CATCATCCTAGGGGGGCCTGTGATCAGCCTTCGGGCAGGGGACCCCCTCAACCTCACCTGCCACGCAGAC
  AATGCCAAGCCTGCGGCTTCCATCATCTGGCTACGTAAAGGAGAGGTCATCAATGGAGCCACCTACTCCA
  AGACCCTGCTTCGAGACGGCAAACGAGAAAGCATTGTCAGCACCCTCTTCATCTCCCCAGGAGACGTGGA
  AAATGGACAGAGTATTGTGTGCCGAGCCACCAACAAAGCCATCCCCGGAGGAAAAGAGACCTCTGTCACC
  ATAGACATCCAGCATCCACCGCTTGTCAACTTGTCCGTGGAACCACAGCCGGTATTGGAGGACAACATCG
  TCACGTTCCACTGCTCTGCAAAGGCCAACCCAGCTGTCACCCAGTACAGGTGGGCCAAACGGGGTCACAT
  CATCAAGGAGGCATCTGGGGAGCTGTATAGGACCACGGTGGACTACACATACTTCTCAGAGCCTGTATCC
  TGTGAAGTAACCAATGCCCTGGGCAGCACCAACCTCAGCCGCACAGTGGATGTATACTTCGGTCCTCGAA
  TGACCTCAGAGCCTCAGTCACTGCTGGTAGATCTGGGCTCCGATGCTGTCTTCAGCTGTGCGTGGATCGG
  CAACCCGTCTCTGACCATCGTGTGGATGAAACGAGGTTCTGGTGTGGTCCTGAGCAATGAAAAGACCCTA
  ACCCTCAAATCTGTCCGCCAAGAGGATGCTGGGAAGTACGTGTGCCGGGCTGTGGTGCCCCGGGTAGGAG
  CTGGGGAGAGAGAGGTGACCTTGACTGTCAATGGACCCCCCATCATCTCCAGCACACAGACCCAGCACGC
  CCTCCACGGAGAGAAGGGCCAGATCAAATGCTTCATCCGGAGCACACCACCGCCTGACCGAATTGCCTGG
  TCCTGGAAGGAGAATGTGCTGGAGTCAGGGACATCAGGGCGCTACACAGTGGAGACGGTGAACACGGAGG
  AGGGAGTCATCTCCACATTGACCATTAGCAACATTGTGCGTGCTGACTTCCAGACCATATACAACTGTAC
  AGCCTGGAACAGCTTTGGCTCTGACACAGAGATCATCCGACTCAAGGAACAAGAGTCTGTACCAATGGCC
  GTCATCATCGGGGTGGCCGTAGGAGCTGGCGTGGCCTTCCTCGTCCTAATGGCAACCATTGTGGCCTTCT
  GCTGTGCCCGTTCCCAGAGAAATCTCAAAGGTGTTGTATCAGCCAAAAATGATATTCGAGTGGAAATTGT
  GCACAAGGAGCCATCTTCTGGCCGGGAGGCTGAGGACCACACCACCATAAAGCAGCTGATGATGGACCGG
  GGTGAATTCCAACAAGACTCGGTGCTGAAACAGCTGGAGGTCCTCAAAGAAGAGGAGAAGGAGTTTCAGA
  ACCTGAAGGACCCCACCAACGGCTACTACAGCGTCAACACCTTCAAAGAACACCATTCAACTCCAACCAT
  CTCCCTGTCCAGCTGCCAGCCAGACCTGCGTCCGACAGGCAAACAGCGTGTGCCCACAGGCATGTCCTTC
  ACCAACATCTACAGCACCTTGAGCGGCCAGGGCCGCCTCTACGACTATGGACAGAGGTTTGTGCTGGGCA
  TGGGCAGCTCTTCCATTGAGCTTTGTGAGCGGGAGTTTCAGAGGGGCTCCCTCAGCGACAGCAGCTCCTT
  CCTGGACACGCAGTGTGACAGCAGCGTCAGCAGCAGCGGCAAGCAAGATGGCTACGTGCAGTTTGACAAG
  GCCAGCAAGGCTTCTGCCTCCTCTTCCCACCATTCCCAGTCCTCTTCCCAGAACTCCGACCCCAGCCGAC
  CCCTGCAGCGGCGGATGCAGACTCACGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222381 representing NM_001190914
  Red=Cloning site Green=Tags(s)
  
  MAKDKFRRMNEGQVYSFSQQPQDQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVG
  NHLSGEHHLKILRAELQDDAVYECQAIQAAIRSRPARLTVLVPPDDPIILGGPVISLRAGDPLNLTCHAD
  NAKPAASIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKETSVT
  IDIQHPPLVNLSVEPQPVLEDNIVTFHCSAKANPAVTQYRWAKRGHIIKEASGELYRTTVDYTYFSEPVS
  CEVTNALGSTNLSRTVDVYFGPRMTSEPQSLLVDLGSDAVFSCAWIGNPSLTIVWMKRGSGVVLSNEKTL
  TLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAW
  SWKENVLESGTSGRYTVETVNTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQESVPMA
  VIIGVAVGAGVAFLVLMATIVAFCCARSQRNLKGVVSAKNDIRVEIVHKEPSSGREAEDHTTIKQLMMDR
  GEFQQDSVLKQLEVLKEEEKEFQNLKDPTNGYYSVNTFKEHHSTPTISLSSCQPDLRPTGKQRVPTGMSF
  TNIYSTLSGQGRLYDYGQRFVLGMGSSSIELCEREFQRGSLSDSSSFLDTQCDSSVSSSGKQDGYVQFDK
  ASKASASSSHHSQSSSQNSDPSRPLQRRMQTHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190914
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190914.1, NP_001177843.1
• RefSeq Size: 3802 bp
• RefSeq ORF: 2202 bp
• Locus ID: 67703
• Cytogenetics: 9 A4
• MW: 80.9 kDa
• Gene Summary: Synaptic adhesion molecule required for the formation of target-specific synapses (PubMed:23637329, PubMed:26575286). Required for formation of target-specific synapses at hippocampal mossy fiber synapses. Required for formation of mossy fiber filopodia, the synaptic structures connecting dentate granule and GABA neurons. Probably acts as a homophilic adhesion molecule that promotes trans-cellular interactions and stabilize mossy fiber filipodia contact and subsequent synapse formation (PubMed:26575286). Required for the coalescence of vomeronasal sensory neuron axons (PubMed:23637329). May be involved in the hematopoietic supportive capacity of stroma cells; the secreted extracellular domain is directly responsible for supporting hematopoietic stem cells (PubMed:12665856).[UniProtKB/Swiss-Prot Function]