Ccdc141 (NM_001025576) Mouse Tagged ORF Clone

CAT#: MR222228

Ccdc141 (Myc-DDK-tagged) - Mouse coiled-coil domain containing 141 (Ccdc141)

Product Images

Circular map for MR222228

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccdc141
• Synonyms: 2610301F02Rik; CAMDI; ENSMUSG00000075261
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222228 representing NM_001025576
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTGCAAAGAAAGTCCCCATGTGGGTGCTTCCACCACCACTGTCAGCTCTGTAGCCGTGCACGCTG
  GGGACTCCAAGATCGTGATCGCCGTTGTCAAGTGTGGCAAATGGGTACGACTCCAACTGGCTGAATCGCA
  GCCCAATCTCCTCGAAATTGGGAGCAGTCAAGATGAAACCAAAAAACTCCTTCACGATCATGAGCTGCTT
  CTGGCCAAGCTTAAGGCCTTGGAAGATCGTGTGTGGGAACTCTTACGGGAAGCAGACCGGACAGCTGAAG
  CAAACAAGGCTCAAAGTCAGGTGTATGACGCCATGGCCCAGACGCTGGGTGAGGCATGGGCAACCCTGGT
  CTCCATGCTTGAAAGAAGAAGGGAGCTCCTTGGGCTGACGTCCGAGTTCTTTCAAAGTGCTTTGGAGTTT
  GCTATAAAAATAGACCAAGCTGAAGCTTTTCTGCAGAATCCTCATGAGTTTGAGAGCACTGAAGCCTTAC
  AGTCACTTCTTCTGCTTCATGACCGACATGCCAAAGAACTCTTAGAAAGATCCCTAGACCTTTTAAACAA
  AAGTCAACAACTGACTGACTTCATAGAAAAATTCAAGTGTGAAGGATCTACTATGAATTCTGAGCTGATC
  CAGGGAGCTCAGAGCAGCTGTCTGAAGATCGACAGCCTCCTTGAACTTCTGCAAGACAGGAGAAGGCAGC
  TCGACAAGTACTTGCAGCAACAGCGGCAGGAATTGTCTCAGGTTCTGCAGTTGTGTCTGTGGGACCAACA
  AGAAAACCAGGTTTCTTCTTGGTTTCAGAAAGCAATAAGAGATCTGCAGGAACAGAGTCTGGGCGCATCA
  CTTTCAGACAACAGAGAGCTAATCTGCAAGCACGAGGACCTGATAGTCAAAGCAAAGGAATGGGATTCGG
  CCGTAGAGAAGCTGAAAAGCCAGGCTTTGGGGATTCTATTGTCCAAGGACTTGGCAGGAAAGGAACATCT
  ACAGCTCTCTAACCAGAAACTGAATCGGCTTCAAGAAGAATTTGGAAGACTCATGGTGGAAAGGAAAGCC
  TGGTTATCGATGGCAAATGACTTTTTTACCAGTGCTAACAAGTCATTTGATGTACTTGGGAAAGTTGAAG
  CTTACCTTAAGCTCCTTAAATCAGAGGGTTTAAGCCTGCCTGTCTTGGCAGCGAAGCATGAGGAATTACA
  CAGAGAAATTAAAGACTCTACAGCCACTGCTCTGCAAAAGGGACGAACCTTAATCAGCCAAGTAGACTCC
  TGCCGCTCTCGGGTGACAGGAATCCACGAGATGATGGGATACATTCAGAACCGAGTGGATTGTCTCACTG
  AACAGTGTACAGCGCATGAGGAATTTGCCCGCAAGAGACAGCAATTAGCAACATCAGTGGACGACTACCT
  AAGGAAGGTGGAAATGTCAATTCAGGAAATCAGGCCCATTCTTGCTACCACATTGGATGTTGCTTCCAGC
  CCTTCTGAATCAGAGAAGATTTTAAATAAATATCTGGAATTGGATATCCAAGTCAAGGAGACAGCACATG
  CATTAGAAGCAGCTGCAAAAATCATGACAGAGAAAAATGAACTTGAACTCAATGAAGTGGCATTGCTTCC
  TCTTAAAGTGAAATGGCTAGAGGAAGAATTAAGCACGCTTGGTCGAAGCATCAGCTGCAGGTCACGGATC
  CTGCAGACGTACGTGGCATTTCGAAAGTCATCCGAGGAGGCAGAGGAGCAGCTTCAGAGCCTAAAGGAAT
  TTTATCTGACTGAAATTCCTTGGAAAGATGAAGACGATGCTGTGGTCAAGTGCCAGTCTAACTCCGCTGA
  GAGGAAATGGCAGCTGTTTTTAAAGAAGAGTTTTTTGACCCAGGACTTGAGCCTCGAGTTTCTGAACTTA
  ATAAACATGGCAAAAGAGAATGAGATATTAAATGTTAAAAATGAAATGCACATTATGGAGAACATTATGG
  AAAAGCAAACAAATGGAAGGGAAGAGCTCAGCCATCTCCGAGTGGCGTGGTACCTCAAAGCTATCGAAGG
  CAAGCCTGCGAGAGAGCAGTGGGAAATGTTCAAAGAGAAACTTACAAAGACTACTCACAGTGTAAAACTT
  CTCCACGAAGTTCTCATGCCTGTCTCTGCGCTTGACCTTGGGGGGAGCCTCCAGAGCACGTCAGATCTGC
  GGAGGAGGTGGATCGCAATGAAGCCTCAGCTCCAGCAACTGCATGAGGATGTTCAGCAAATCACGAAAGA
  GTGGGAAGTGTTAAGCAGTCAGGGAGCTCCTCTGAAGGAGAAGGCTGAACAGCTGAAGGACCTTGTCCAT
  CTCCACCGGAGGCAGCGAGAGCGAATCCAGGAATATGAGGAAATCCTATACAAGACAGTCCAGTTCCACC
  AGGTCAAGGAAGAGCTGGTCCATCTCATCAAACCAAGAGAACTGGAACTTCTAGCACAGCCCATGGAACT
  GGCAAGTTCTGAGGAGGTCCAGATGCAACTGGGCCGTTCGCAAGAAAGGCGGGCACATGTAGACCATCTC
  CATCAGCTGGCCCTGACCTTAGGAGTGGACATCATTTCATCCGTGCAACAACCTAATTGCTCTAATATTT
  CTGCAAAGAACCTGCAGCAGCAGCTGGAGGCCCTTGAACTGGAGAGCAGGAGTTGGAGTGCCCAAGCCAA
  AGAACATGAGCGCGTCCTGTCCTGCAGCTTGGAGTACTGCACTGCACGTGATGAGATCAGTGAGCTCAAA
  GAGTCATTCAAAGACATAAAAAAGAAATTCAACAATCTGAAGTTTAATTACTCCAAGAAAAATGAGAAAT
  CTCGAAATTTAAAGACTCTTCAGTATCAAATCCAACAAGTGGATACATATGCTGAAAAAATTCAGGCTCT
  AAGGAAGAAAATGGAAAAAGTTAATAATAAGACATCTGATTCATTCTTAAGTTATCCAAGTAATAAAGCT
  AACATGCTTTCGGAAGCCATGGAGGATTTGAAAAAGAACGTGGATGACTTTGACAAAGTTGTGACAGATT
  ACAAAATGAATTTGGACCTGACTGAGCATCTTCAGGAAGTAATAGAAGAGTGTAACTTTTGGTATGAAGA
  TGCAAGTGCCACCGTTGTGAGAGTTGGGAAGTATTCCATGGAGTGCCAAACAAGGGAAGCTGTGGACATC
  CTCCACAGGCAGTTCAATAAGTTTATTACTCCCTCGGTGCCCCAGCAAGAAGAAAGGATTCAGGAGGTCA
  TTGACCTTGCTCAGCGCTTATATGGTTTGGAAGAGGGGCAGAAATATGCAGAGAAGATTGTCACAAGACA
  CAAGGAGATTCTTGAATCTATTACTGAATTATGTGGGTCTCTCGTGGAACTTAAAGAAAAACTGATGCAG
  GGAGAGGTTCCAAAGATGAATTCAGATTTGGAAGATTTCCATGACAATTGCATTGATCTGCTAAAGGGGC
  CAGGGAAGGATGACCAGAAAACATTCAGTGAAGAAAGGAATGAGGGGCAGGTGCAGGGAGCAGATGTATT
  GGCCGTCAATGGAACAAGAGAAGATGGGCTTCCCATGGACTTGAGGCGGACCTCCTCTGACAAAGAGGAC
  AGCGCCCAGGGTCTGATCCTTCCAGAAGACACACTATCTGGAGAAGAATCTGAGTGTATCTCATCCGATG
  ACATCTCTTTGCCTCCACTGCCAGTGAGCCCCGAGTCCCCTCTGGCACCATCAGACATGGAGGTAGAAGA
  ACTTACCAGCTCCTCTGCCCTCGCCCTGCACATCAGCGGCTACAGGATGCATCCAGGGACAGGTGGTCTA
  GGAAAGGCCCAGGAGTCTGCTCTTCCCTCACCCATTGCCTTTGCGGATGGGGGCCATAGTAAGAAAGACA
  CATTTACAAGTCATTTTGAGAGGCCCTATCCACAGTTGAAAGCTGAATCCTCATTAGCCTCCAGAGGATC
  AGCAGAGATGAGTACCAAGTTGCACATCAATGTGAAATGTCCAGCGAGCATGCCCCATGAAGTGCACGAC
  AAAGCTTTACAGCAATGCTCCCAGGCTCGGGAGAGCACACTAGAAATGCAGGAAAAAGTGCATGCTGATT
  CTAACGTCACTAAAACCCAAGACAGGCTGCATGCTGCCCTTGATGTGTCCCCTGGCCTCGGCTCTCAACC
  AGACACCAGCGAGAGCCATCAGAGGCGAGTGGGTCCCCAAGGAAACAAGAAAAACTCATCCGCAGAAAAC
  AGCGTGGTCAGCCTAGCTGGCCAGGCACCTCATTTCTCCAGGCTGCTGTCCAATGTAACTGTCATGGAAG
  GTTCCCCAGTGACTTTGGAAGTCGAAGTAACAGGATTCCCAGAGCCTACACTGACATGGTTCAAGAAGGG
  CCAGAAACTGTGTGCAGATGGACACTTACAGGTCTTGCACAAGGACACAAAGCATTCGGTGTTCATTCCG
  AAGGTATGTGAGGCAGATGCTGGCCTCTATGTGGCTCAAGCCCAAAACTCTAGTGGCACCCTGTCTTCCA
  AAGCCATCCTCCATGTGACAGGTAACCATGGGCCACCAATTACAAGACTAAACTGGATTATGCTGTGTAT
  CATCTATGTCAGTGTATCAGTAATATACTGGCTACTTACACGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222228 representing NM_001025576
  Red=Cloning site Green=Tags(s)
  
  MSCKESPHVGASTTTVSSVAVHAGDSKIVIAVVKCGKWVRLQLAESQPNLLEIGSSQDETKKLLHDHELL
  LAKLKALEDRVWELLREADRTAEANKAQSQVYDAMAQTLGEAWATLVSMLERRRELLGLTSEFFQSALEF
  AIKIDQAEAFLQNPHEFESTEALQSLLLLHDRHAKELLERSLDLLNKSQQLTDFIEKFKCEGSTMNSELI
  QGAQSSCLKIDSLLELLQDRRRQLDKYLQQQRQELSQVLQLCLWDQQENQVSSWFQKAIRDLQEQSLGAS
  LSDNRELICKHEDLIVKAKEWDSAVEKLKSQALGILLSKDLAGKEHLQLSNQKLNRLQEEFGRLMVERKA
  WLSMANDFFTSANKSFDVLGKVEAYLKLLKSEGLSLPVLAAKHEELHREIKDSTATALQKGRTLISQVDS
  CRSRVTGIHEMMGYIQNRVDCLTEQCTAHEEFARKRQQLATSVDDYLRKVEMSIQEIRPILATTLDVASS
  PSESEKILNKYLELDIQVKETAHALEAAAKIMTEKNELELNEVALLPLKVKWLEEELSTLGRSISCRSRI
  LQTYVAFRKSSEEAEEQLQSLKEFYLTEIPWKDEDDAVVKCQSNSAERKWQLFLKKSFLTQDLSLEFLNL
  INMAKENEILNVKNEMHIMENIMEKQTNGREELSHLRVAWYLKAIEGKPAREQWEMFKEKLTKTTHSVKL
  LHEVLMPVSALDLGGSLQSTSDLRRRWIAMKPQLQQLHEDVQQITKEWEVLSSQGAPLKEKAEQLKDLVH
  LHRRQRERIQEYEEILYKTVQFHQVKEELVHLIKPRELELLAQPMELASSEEVQMQLGRSQERRAHVDHL
  HQLALTLGVDIISSVQQPNCSNISAKNLQQQLEALELESRSWSAQAKEHERVLSCSLEYCTARDEISELK
  ESFKDIKKKFNNLKFNYSKKNEKSRNLKTLQYQIQQVDTYAEKIQALRKKMEKVNNKTSDSFLSYPSNKA
  NMLSEAMEDLKKNVDDFDKVVTDYKMNLDLTEHLQEVIEECNFWYEDASATVVRVGKYSMECQTREAVDI
  LHRQFNKFITPSVPQQEERIQEVIDLAQRLYGLEEGQKYAEKIVTRHKEILESITELCGSLVELKEKLMQ
  GEVPKMNSDLEDFHDNCIDLLKGPGKDDQKTFSEERNEGQVQGADVLAVNGTREDGLPMDLRRTSSDKED
  SAQGLILPEDTLSGEESECISSDDISLPPLPVSPESPLAPSDMEVEELTSSSALALHISGYRMHPGTGGL
  GKAQESALPSPIAFADGGHSKKDTFTSHFERPYPQLKAESSLASRGSAEMSTKLHINVKCPASMPHEVHD
  KALQQCSQARESTLEMQEKVHADSNVTKTQDRLHAALDVSPGLGSQPDTSESHQRRVGPQGNKKNSSAEN
  SVVSLAGQAPHFSRLLSNVTVMEGSPVTLEVEVTGFPEPTLTWFKKGQKLCADGHLQVLHKDTKHSVFIP
  KVCEADAGLYVAQAQNSSGTLSSKAILHVTGNHGPPITRLNWIMLCIIYVSVSVIYWLLTR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025576
• ORF Size: 4593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025576.3, NP_001020747.2
• RefSeq Size: 6329 bp
• RefSeq ORF: 4596 bp
• Locus ID: 545428
• Cytogenetics: 2 C3
• MW: 173.6 kDa