Zic2 (NM_009574) Mouse Tagged ORF Clone

CAT#: MR222078

Zic2 (Myc-DDK-tagged) - Mouse zinc finger protein of the cerebellum 2 (Zic2)

Product Images

Circular map for MR222078

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zic2
• Synonyms: HPE5; Ku
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222078 representing NM_009574
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCTGGACGCGGGGCCGCAGTTCCCGGCCATCGGGGTGGGCAGCTTCGCGCGCCACCACCACCACT
  CGGCCGCGGCAGCGGCGGCTGCGGCGGCCGAGATGCAGGACCGCGAGCTGAGCCTGGCGGCAGCTCAGAA
  CGGCTTCGTGGACTCGGCCGCGGCGCACATGGGCGCCTTCAAGCTCAACCCCGGGGCACACGAACTGTCT
  CCTGGTCAGAGTTCGGCGTTCACGTCGCAAGGTCCGGGTGCTTACCCGGGTTCGGCTGCTGCAGCCGCTG
  CGGCCGCGGCTCTAGGGCCCCACGCCGCACACGTTGGCTCCTATTCGGGGCCTCCCTTTAATTCCACCCG
  GGACTTCCTGTTCCGCAGCCGGGGCTTCGGGGACTCGGCGCCGGGAGGCGGGCAGCACGGGCTCTTCGGA
  CCCGGCGCCGGCGGCCTTCACCACGCGCACTCGGACGCGCAGGGCCACCTTCTTTTCCCTGGCCTCCCGG
  AGCAGCACGGGCCGCACGCCTCGCAGAACGTGCTCAATGGGCAAATGCGCCTAGGGCTGCCGGGCGAGGT
  GTTCGGGCGCTCGGAGCAATACCGCCAAGTGGCCAGCCCGCGGACCGACCCCTACTCGGCGGCGCAGCTC
  CACAACCAGTACGGCCCTATGAATATGAACATGGGGATGAACATGGCAGCGGCCGCAGCCCACCACCACC
  ATCACCACCACCACCCTGGTGCCTTTTTCCGCTACATGCGGCAGCAGTGCATCAAGCAAGAGCTCATCTG
  CAAGTGGATCGACCCGGAGCAGCTAAGCAATCCCAAGAAAAGCTGCAACAAAACTTTCAGCACCATGCAC
  GAGCTGGTGACCCACGTCTCTGTGGAGCACGTCGGCGGCCCGGAACAGAGCAACCACGTCTGCTTCTGGG
  AGGAGTGTCCACGCGAGGGCAAACCCTTTAAGGCCAAATACAAACTGGTCAACCACATCCGCGTGCACAC
  TGGCGAGAAGCCCTTCCCCTGTCCTTTCCCGGGCTGTGGCAAGGTCTTCGCACGCTCCGAGAACCTCAAG
  ATCCACAAAAGAACTCATACAGGGGAGAAACCTTTCCAGTGTGAGTTCGAGGGCTGTGACCGGCGCTTCG
  CCAACAGCAGCGACAGGAAGAAGCACATGCATGTCCACACCTCAGATAAGCCCTATCTCTGCAAGATGTG
  TGACAAGTCCTACACGCATCCCAGCTCGTTGCGGAAGCACATGAAGGTCCATGAGTCCTCCCCTCAGGGC
  TCCGAGTCCTCCCCGGCTGCCAGCTCTGGCTACGAGTCGTCCACACCCCCGGGGTTGGTGTCCCCCAGCG
  CAGAGCCACAAAGCAGCTCCAACCTGTCCCCAGCAGCGGCGGCAGCAGCAGCAGCGGCTGCGGCAGCAGC
  GGCCGCGGTGTCCGCAGTGCACCGAGGCGCGGGTTCTGGCAGCAGCGGCTCCGGAGGGGGCTCGGCGGCC
  GGCAGCGGTGGGGGCGGCGGCGGGGCGGGCGGCGGGGGCGGCGGCAGCTCTGGCGGGGGCAGCGGGACAA
  CCGGAGGCCATAGCGGCCTCTCCTCCAACTTCAATGAATGGTACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222078 representing NM_009574
  Red=Cloning site Green=Tags(s)
  
  MLLDAGPQFPAIGVGSFARHHHHSAAAAAAAAAEMQDRELSLAAAQNGFVDSAAAHMGAFKLNPGAHELS
  PGQSSAFTSQGPGAYPGSAAAAAAAAALGPHAAHVGSYSGPPFNSTRDFLFRSRGFGDSAPGGGQHGLFG
  PGAGGLHHAHSDAQGHLLFPGLPEQHGPHASQNVLNGQMRLGLPGEVFGRSEQYRQVASPRTDPYSAAQL
  HNQYGPMNMNMGMNMAAAAAHHHHHHHHPGAFFRYMRQQCIKQELICKWIDPEQLSNPKKSCNKTFSTMH
  ELVTHVSVEHVGGPEQSNHVCFWEECPREGKPFKAKYKLVNHIRVHTGEKPFPCPFPGCGKVFARSENLK
  IHKRTHTGEKPFQCEFEGCDRRFANSSDRKKHMHVHTSDKPYLCKMCDKSYTHPSSLRKHMKVHESSPQG
  SESSPAASSGYESSTPPGLVSPSAEPQSSSNLSPAAAAAAAAAAAAAAAVSAVHRGAGSGSSGSGGGSAA
  GSGGGGGGAGGGGGGSSGGGSGTTGGHSGLSSNFNEWYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009574
• ORF Size: 1587 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009574.3, NP_033600.3
• RefSeq Size: 2967 bp
• RefSeq ORF: 1590 bp
• Locus ID: 22772
• Cytogenetics: 14 65.97 cM
• MW: 55.3 kDa