Ccm2 (NM_001190344) Mouse Tagged ORF Clone

CAT#: MR222077

Ccm2 (Myc-DDK-tagged) - Mouse cerebral cavernous malformation 2 homolog (human) (Ccm2), transcript variant 3

Product Images

Circular map for MR222077

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccm2
• Synonyms: BC029157
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222077 representing NM_001190344
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGAGTATTTAGGTCAGTTAACATCCATTCCCGGCTACCTGAACCCTTCCAGTAGGACGGAAA
  TCCTGCATTTCATAGACAAGGCAAAGCGGTCCCACCAGCTTCCTGGGCACCTGACTCAGGAGCACGATGC
  TGTGCTCAGTCTGTCTGCCTACAATGTCAAGTTGGCCTGGAGGGACGGGGAGGACATTATCCTCAGGGTG
  CCCATCCATGATATCGCTGCTGTCTCCTATGTCCGAGATGATGCTGCACACCTGGTGGTCCTGAAGACAG
  CCCAGGACCCAGGCATCTCTCCCAGCCAGAGTCTGTGTGCAGAAAGTTCTAGAGGCCTCAGCGCAGGTTC
  CTTGTCAGAAAGTGCAGTGGGGCCAGTAGAGGCATGTTGCCTGGTCATCATGGCCACAGAGAGCAAGGTC
  GCCGCTGAAGAGTTGTGCTCCCTGCTCAGCCAGGTCTTCCAGATTGTTTACACGGAGTCCACCATCGACT
  TTCTGGACCGAGCAATATTTGATGGGGCTTCCACACCTACCCACCACCTGTCGCTGCACAGTGATGACTC
  TTCCACGAAAGTGGACATGAAGGACAGTTACGATGCTGACGCCAGCACCTTCTGCTTCCCGGACTCTGGG
  GATGTGGGAGGCCTGCCGCCCTTACCCTTCTGCATGCAGACATCACCCCATAGCAAGACTGTCAGTGAGA
  GCGAGCTGAGCACCAGCGCCACGGAACTGCTGCAGGACTACATGCTCACGTTACGTACGAAGCTGTCATC
  ACAGGAGATCCAGCAGTTCGCAGCTCTGCTACATGAGTACCGCAATGGGGCCTCTATCCATGAGTTTTGC
  ATCAGCCTGCGGCAGCTCTATGGGGACAGCCGCAAGTTCCTGCTACTTGGTCTCAGACCCTTCATACCTG
  AGAAGGACAGTCAGCACTTTGAAAACTTCCTGGAGACCATTGGCGTGAAAGACGGCCGTGGCATCATCAC
  TGACAGCTTTGGTAGGCATCGTCGTGCCCTGAGTACCACCTCCACATCCACCATCAATGGGAACAGGACC
  ACAGGCAGCCCTGATGACCGCTCTGCGCCCTCAGAGGGGGATGAGTGGGACCGCATGATTTCAGACATCA
  GTAGTGATATTGAAGCGCTAGGCTGCAGCATGGACCAGGACTCAGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222077 representing NM_001190344
  Red=Cloning site Green=Tags(s)
  
  MENEYLGQLTSIPGYLNPSSRTEILHFIDKAKRSHQLPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRV
  PIHDIAAVSYVRDDAAHLVVLKTAQDPGISPSQSLCAESSRGLSAGSLSESAVGPVEACCLVIMATESKV
  AAEELCSLLSQVFQIVYTESTIDFLDRAIFDGASTPTHHLSLHSDDSSTKVDMKDSYDADASTFCFPDSG
  DVGGLPPLPFCMQTSPHSKTVSESELSTSATELLQDYMLTLRTKLSSQEIQQFAALLHEYRNGASIHEFC
  ISLRQLYGDSRKFLLLGLRPFIPEKDSQHFENFLETIGVKDGRGIITDSFGRHRRALSTTSTSTINGNRT
  TGSPDDRSAPSEGDEWDRMISDISSDIEALGCSMDQDSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190344
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190344.1, NP_001177273.1
• RefSeq Size: 1760 bp
• RefSeq ORF: 1170 bp
• Locus ID: 216527
• Cytogenetics: 11 A1
• MW: 43 kDa
• Gene Summary: Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions. May also function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock.[UniProtKB/Swiss-Prot Function]