Tnnt3 (NM_001163670) Mouse Tagged ORF Clone

CAT#: MR221587

Tnnt3 (Myc-DDK-tagged) - Mouse troponin T3, skeletal, fast (Tnnt3), transcript variant 8

Product Images

Circular map for MR221587

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnnt3
• Synonyms: fTnT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221587 representing NM_001163670
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACGAGGAAACTGAACAAGTTGAGGAGGAGGCCCAAGAGGAAGAGGAAGTCCAAGAAGAGGAGA
  AACCAAGACCCAAACTTACTGCTCCTAAGATCCCGGAAGGGGAGAAAGTAGACTTTGATGACATCCAGAA
  GAAGCGTCAAAACAAGGACCTCATGGAGCTCCAAGCCCTCATTGACAGCCACTTTGAAGCTAGAAAGAAA
  GAAGAAGAGGAGCTAATCGCACTTAAAGAAAGAATTGAGAAGCGCCGTGCAGAGAGGGCTGAGCAGCAAA
  GAATTCGCGCTGAGAAGGAGCGGGAACGCCAGAACAGATTGGCGGAGGAGAAGGCCAGAAGAGAGGAGGA
  GGATGCCAAGAGGAGAGCTGAAGATGACATGAAGAAGAAAAAGGCTCTGTCCTCCATGGGTGCCAACTAC
  AGCAGCTACCTGGCCAAGGCTGACCAGAAGAGAGGCAAGAAACAGACAGCCCGGGAAATGAAAAAGAAGA
  TTCTTGCCGAAAGGCGCAAGCCTCTGAACATTGACCATCTTAGCGATGACAAGCTGAGGGACAAGGCCAA
  GGAACTCTGGGATACCTTGTACCAACTGGAGACTGACAAATTCGAGTTTGGGGAGAAGCTGAAACGTCAG
  AAATATGATATCACCACCCTCAGGAGCCGCATTGACCAAGCCCAGAAGCACAGCAAGAAGGCCGGTGCCA
  CAGCCAAGGGCAAAGTCGGTGGGCGCTGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221587 representing NM_001163670
  Red=Cloning site Green=Tags(s)
  
  MSDEETEQVEEEAQEEEEVQEEEKPRPKLTAPKIPEGEKVDFDDIQKKRQNKDLMELQALIDSHFEARKK
  EEEELIALKERIEKRRAERAEQQRIRAEKERERQNRLAEEKARREEEDAKRRAEDDMKKKKALSSMGANY
  SSYLAKADQKRGKKQTAREMKKKILAERRKPLNIDHLSDDKLRDKAKELWDTLYQLETDKFEFGEKLKRQ
  KYDITTLRSRIDQAQKHSKKAGATAKGKVGGRWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163670
• ORF Size: 732 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163670.1, NP_001157142.1
• RefSeq Size: 1024 bp
• RefSeq ORF: 735 bp
• Locus ID: 21957
• Cytogenetics: 7 87.94 cM
• MW: 29.4 kDa
• Gene Summary: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.[UniProtKB/Swiss-Prot Function]