Tnnt3 (NM_001163667) Mouse Tagged ORF Clone

CAT#: MR221584

Tnnt3 (Myc-DDK-tagged) - Mouse troponin T3, skeletal, fast (Tnnt3), transcript variant 3

Product Images

Circular map for MR221584

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnnt3
• Synonyms: fTnT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221584 representing NM_001163667
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACGAGGAAACTGAACAAGTTGAGGAACAATACGAAGAGGAAGAGGAGGCCCAAGAGGAAGAAG
  TCCAGGAGGAAGAGGAAGTCCAAGAAGATGCTGTCGCCGAGGAGGAGCGGGAGGAGGACGAGGAAGAGGA
  GAAACCAAGACCCAAACTTACTGCTCCTAAGATCCCGGAAGGGGAGAAAGTAGACTTTGATGACATCCAG
  AAGAAGCGTCAAAACAAGGACCTCATGGAGCTCCAAGCCCTCATTGACAGCCACTTTGAAGCTAGAAAGA
  AAGAAGAAGAGGAGCTAATCGCACTTAAAGAAAGAATTGAGAAGCGCCGTGCAGAGAGGGCTGAGCAGCA
  AAGAATTCGCGCTGAGAAGGAGCGGGAACGCCAGAACAGATTGGCGGAGGAGAAGGCCAGAAGAGAGGAG
  GAGGATGCCAAGAGGAGAGCTGAAGATGACATGAAGAAGAAAAAGGCTCTGTCCTCCATGGGTGCCAACT
  ACAGCAGCTACCTGGCCAAGGCTGACCAGAAGAGAGGCAAGAAACAGACAGCCCGGGAAATGAAAAAGAA
  GATTCTTGCCGAAAGGCGCAAGCCTCTGAACATTGACCATCTTAGCGATGACAAGCTGAGGGACAAGGCC
  AAGGAACTCTGGGATACCTTGTACCAACTGGAGACTGACAAATTCGAGTTTGGGGAGAAGCTGAAACGTC
  AGAAATATGATATCACCACCCTCAGGAGCCGCATTGACCAAGCCCAGAAGCACAGCAAGAAGGCCGGTGC
  CACAGCCAAGGGCAAAGTCGGTGGGCGCTGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221584 representing NM_001163667
  Red=Cloning site Green=Tags(s)
  
  MSDEETEQVEEQYEEEEEAQEEEVQEEEEVQEDAVAEEEREEDEEEEKPRPKLTAPKIPEGEKVDFDDIQ
  KKRQNKDLMELQALIDSHFEARKKEEEELIALKERIEKRRAERAEQQRIRAEKERERQNRLAEEKARREE
  EDAKRRAEDDMKKKKALSSMGANYSSYLAKADQKRGKKQTAREMKKKILAERRKPLNIDHLSDDKLRDKA
  KELWDTLYQLETDKFEFGEKLKRQKYDITTLRSRIDQAQKHSKKAGATAKGKVGGRWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163667
• ORF Size: 804 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163667.1, NP_001157139.1
• RefSeq Size: 1096 bp
• RefSeq ORF: 807 bp
• Locus ID: 21957
• Cytogenetics: 7 87.94 cM
• MW: 32.4 kDa
• Gene Summary: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.[UniProtKB/Swiss-Prot Function]