Blzf1 (NM_001160208) Mouse Tagged ORF Clone

CAT#: MR221124

Blzf1 (Myc-DDK-tagged) - Mouse basic leucine zipper nuclear factor 1 (Blzf1), transcript variant 2

Product Images

Circular map for MR221124

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Blzf1
• Synonyms: 1700030G05Rik; Blzf1l; Golgin-45; Jem-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221124 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAAATGACTACCCTTAAAAGTTCGGAAAACAAAGGCATCCTTACTTCGACCCCAATCCGAGGAG
  CAGGAGATGGAATGGAAACGGAGGAGCCCCCTAAGTCTGTTGAAGTCACCCACGGAGTCCAACCCATAAA
  TCAACATGTCCTTCCAAGTCCACGGAAGAAAGTGTCCTCAGACAGCCCGGGCGTTCTTCAGCTGGGGAAG
  ATTCTTAATGAAAGAACAGTGGAAGTTGAAGCTGTCAGAATATTTGTTCCCAAAGCTGCTATCACTCACG
  ACATCCCCACCAAGAACACAAAGGTTAAGTCCCTAGGACATCACAGAGAAGAACTCCACAATCAGGCAGA
  AGTGGTCACAGACCCAAGGAAGGAACTCTCAGAGGTAAAGAAGGTTTTGGAAAAGCTCAAGAATTCTGAA
  AGGAGGCTACTTCAGGACAAAGAAGGCCTTTCCAATCAGCTCCGAGTACAGACAGAGATAAATCGTGAGT
  TAAAGAAGTTACTCGTGGCTTCTGTTGGGGATGACCCGCAGTATCACTTTGAACGTCTAGCCCGGGAGAA
  GAACCAGCTCATCTTAGAAAATGAAGCACTAGGTCGGAACACAGCCCAGCTTTCTGAACAGCTGGAACGG
  ATGTCAATACAGTGCGATGTCTGGAGAAGTAAATTCCTTGCAAGCAGGGTAATGGCAGATGAGTTAACAA
  ACTTCAGAGTGGTTTTACAGCGTCAGAACAGAGATGCCCAGAGCGCTATCCAGGATCTCCTGAGTGAACG
  AGAGCAGTTTCGTCAGGAAATGACCTCTACCCAAAAATTTTTGGAAGAACTCTTGGTCTCCTTACAGTGG
  GGGAGAGAGCAGACATACTCCCCTAACACACAGCCCCATAGCACGGCAGACTTAGCACTCACGAACCATG
  GACTGGCACAAGCAATACATGCCCATCTGTTGGGGAATGTTGGCATTCGCCATCAAAAGAAGATTCCAAC
  AACAGTCGAATTCTGCAGTACCCCAGCTGAGAAAATGGCTGAAAAGGTTCTGCGGAATTTGGATCCAGTT
  GCCTGTACAGAGAGCTCTCCGGACAACCAATTTGCTGAATCTTCACCAACCACATTACTCACTACAAAGA
  AAAATATTGGACGTTTCCATCCCTATACCAGATATGAAAATATAACTTTCAATTGCTGCAATCACTGCCA
  GGGGGAGCTCATTGCTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221124 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKMTTLKSSENKGILTSTPIRGAGDGMETEEPPKSVEVTHGVQPINQHVLPSPRKKVSSDSPGVLQLGK
  ILNERTVEVEAVRIFVPKAAITHDIPTKNTKVKSLGHHREELHNQAEVVTDPRKELSEVKKVLEKLKNSE
  RRLLQDKEGLSNQLRVQTEINRELKKLLVASVGDDPQYHFERLAREKNQLILENEALGRNTAQLSEQLER
  MSIQCDVWRSKFLASRVMADELTNFRVVLQRQNRDAQSAIQDLLSEREQFRQEMTSTQKFLEELLVSLQW
  GREQTYSPNTQPHSTADLALTNHGLAQAIHAHLLGNVGIRHQKKIPTTVEFCSTPAEKMAEKVLRNLDPV
  ACTESSPDNQFAESSPTTLLTTKKNIGRFHPYTRYENITFNCCNHCQGELIAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160208
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160208.1, NP_001153680.1
• RefSeq Size: 3927 bp
• RefSeq ORF: 1212 bp
• Locus ID: 66352
• UniProt ID: Q8R2X8
• Cytogenetics: 1 H2.2
• MW: 45.6 kDa
• Gene Summary: Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.[UniProtKB/Swiss-Prot Function]