Cnot6l (NM_178854) Mouse Tagged ORF Clone

CAT#: MR221100

Cnot6l (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 6-like (Cnot6l), transcript variant 2

Product Images

Circular map for MR221100

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnot6l
• Synonyms: 4932442K20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221100 representing NM_178854
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTAATAGGGATGCCAAAGGAAAAATATGATCCTCCAGATCCTCGCAGAATTTACACCATCATGT
  CCGCAGAGGAGGTAGCCAATGGGAAAAAATCTCACTGGGCAGAGTTAGAGATCTCGGGTAGAGTGCGGAG
  CTTAAGTACATCACTCTGGTCATTAACACACTTGACAGCACTGCACCTAAATGACAATAACCTTGCTCGC
  ATTCCACCTGATATTGCCAAGCTTCATAATCTGGTTTACCTGGATCTGTCATCCAATAAACTCAGAAGTT
  TACCAGCAGAACTAGGAAACATGGTGTCTCTCAGGGAATTGCTTTTAAATGACAATTATTTACGGGTGTT
  GCCTTATGAACTTGGCCGGCTCTTCCAGCTACAAACTCTAGGTTTAACAGGCAATCCTTTATCACAGGAT
  ATTATGAGCTTATACCAGGACCCAGATGGAACCCGAAAGCTACTGAACTTCATGCTTGACAATCTTGCAG
  TTCATCCAGAGCAGCTTCCTCCGAGGCCATGGATTACATTAAAAGAACGAGACCAAATTCTGCCATCAGC
  ATCATTCACGGTTATGTGTTACAATGTGTTATGTGATAAATATGCTACCAGGCAGCTATATGGCTATTGT
  CCGTCCTGGGCATTAAACTGGGAATACAGGAAAAAGGGAATTATGGAAGAAATTGTTAACTGGGACGCAG
  ATATCATTAGTCTTCAGGAAGTGGAAACAGAGCAATACTTTACTCTCTTTCTGCCAGCATTGAAGGATCG
  TGGATATGATGGATTTTTTTCTCCAAAGTCACGTGCCAAAATCATGTCTGAGCAGGAAAGAAAGCATGTG
  GATGGTTGTGCAATATTCTTCAAAACAGAAAAATTTACATTGGTGCAGAAGCATACAGTGGAATTCAACC
  AGGTAGCAATGGCAAATTCAGATGGATCCGAAGCAATGCTAAACAGAGTAATGACGAAAGATAACATTGG
  CGTTGCTGTGGTGTTAGAGGTCCACAAGGAGCTTTTTGGAACAGGTATGAAGCCTATTCATGCTGCAGAC
  AAACAGCTGCTTATAGTGGCAAATGCCCACATGCATTGGGACCCAGAGTATTCTGATGTGAAACTTATTC
  AGACCATGATGTTTGTCTCAGAGGTTAAAAACATTCTGGAGAAAGCCTCAAGTAGGCCTGGCAGCCCAAC
  TGCAGATCCCAATTCCATCCCGCTGGTGCTATGTGCAGATCTTAACTCATTGCCAGATTCAGGTGTTGTG
  GAATATTTAAGCAACGGTGGAGTAGCTGACAACCATAAAGACTTCAAGGAACTAAGGTACAATGAGTGTC
  TTATGAACTTCAGCTGTAGTGGAAAGAATGGAAGCTCAGAAGGGAGAATCACACATGGCTTCCAACTTAA
  GAGCGCCTATGAAAATAACTTGATGCCTTATACCAATTACACCTTTGATTTCAAAGGTGTGATTGACTAC
  ATTTTCTATTCCAAGACTCATATGAACGTGCTTGGTGTCCTGGGGCCTTTAGATCCTCAATGGCTGGTTG
  AGAACAACATCACTGGGTGTCCACACCCTCACATCCCTTCAGACCACTTCTCACTGTTAACACAACTTGA
  ACTCCACCCTCCACTCCTGCCTCTTGTCAATGGTGTTCACTTGCCTAATCGGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221100 representing NM_178854
  Red=Cloning site Green=Tags(s)
  
  MRLIGMPKEKYDPPDPRRIYTIMSAEEVANGKKSHWAELEISGRVRSLSTSLWSLTHLTALHLNDNNLAR
  IPPDIAKLHNLVYLDLSSNKLRSLPAELGNMVSLRELLLNDNYLRVLPYELGRLFQLQTLGLTGNPLSQD
  IMSLYQDPDGTRKLLNFMLDNLAVHPEQLPPRPWITLKERDQILPSASFTVMCYNVLCDKYATRQLYGYC
  PSWALNWEYRKKGIMEEIVNWDADIISLQEVETEQYFTLFLPALKDRGYDGFFSPKSRAKIMSEQERKHV
  DGCAIFFKTEKFTLVQKHTVEFNQVAMANSDGSEAMLNRVMTKDNIGVAVVLEVHKELFGTGMKPIHAAD
  KQLLIVANAHMHWDPEYSDVKLIQTMMFVSEVKNILEKASSRPGSPTADPNSIPLVLCADLNSLPDSGVV
  EYLSNGGVADNHKDFKELRYNECLMNFSCSGKNGSSEGRITHGFQLKSAYENNLMPYTNYTFDFKGVIDY
  IFYSKTHMNVLGVLGPLDPQWLVENNITGCPHPHIPSDHFSLLTQLELHPPLLPLVNGVHLPNRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178854
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178854.4, NP_849185.2
• RefSeq Size: 3171 bp
• RefSeq ORF: 1668 bp
• Locus ID: 231464
• UniProt ID: Q8VEG6
• Cytogenetics: 5 E3
• MW: 63.5 kDa
• Gene Summary: Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).[UniProtKB/Swiss-Prot Function]