Nwd2 (NM_177006) Mouse Tagged ORF Clone

CAT#: MR220859

3110047P20Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 3110047P20 gene (3110047P20Rik)

Product Images

Circular map for MR220859

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nwd2
• Synonyms: 3110047P20Rik; B830017A01Rik; Hn1-ps2; mKIAA1239
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220859 representing NM_177006
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCCGGCCGGTGCGGGCACCAAGCTGCCCTGTCCCCGGGACTCCGCGCTCCGGCGGGCGGCGTTCT
  CTGGTAATCTCACCGCCCTTCCTTCTCACCTGGTACCCGCCGGTCGCAGCGTCCGGGTCTTCATCAGCGC
  CAACCCTGAAGATACAGGAGCGGAACGGCAGGCACTAAGAGAAACTGTGTATCCGAAACTGAGAGAATTC
  TGCAGAGAAAACTATGGGTTGGAATTTCAGGTCATAGATCTGTACTGGGGAATTGAGGAAGATGAATGGG
  ACAGCCCCGAACTGCAGAAGATGCGCATGAAGCTGCTGGAGGAATGCTTGAAAACTTCTGCAGGTCCATG
  CTTTGTTGGCCTCTTAGGTGAAAAATATGGGAACATTCGAATCCCTGGGGAAGTAGAAGCTTCAGAGTTT
  GAAATGATTTTGGATGCTGCTGTCGAGGCAAAATTAGAGACCAAGTTACTGGAAGATTGGTACTGTCGGG
  ATGAGAACTCTGTGCCAGCAGCCTATTACCTCAGACCCAGGCTGGAGGTGCCAAGAAGCAACAAAAACTC
  AACACAGCCTTCTGCCAGTAGTGAACAGGAGAGGCCATGGCAAGAAATATCGGATGAAATCAAAACGATA
  TTTAAAGCTGCTGTCAAACTGCTGCATGAACAGGGGAAAATGAAGCAAAGTCAGGCTAAGAGGTACCTGT
  TCTCAGCTATCGAGGATGAGTTTGACTTTGCTCTAGGCAAGCAAACTCCAGCCTTCCTGAAGAAGTGTGT
  TTGCTACATCAGAAAGATTGCTAACATCGAACGCTTCGTGAAAATCCCAGAGATGGGGAAATACATGGAC
  ATAACCGGAACAGACCCAAGAATCGTCCGTGACCCAGAAGCTCAAGAGAAGCTGATAAAACTCAGGGATG
  AATTCATTCCTACCATTGTTGCATCCTCTAATTTGAGAGTGTACACATCTGTCACTCACTGTGACATGAA
  ACTGGGCTATTCCCAAGAGATAGAAAACCATTACATAGAAGGACTTGGTAAGCAATTTTATGAAGACATG
  ATTGATATAATTCAGGCAACAGTACAGCAGAACTTTGACACGGAGACTGATACGCTGTACGATGAAATCC
  TGCAGCACTCCTCACTGTGTAAAACATATGCCTCCTTCTATGAATACAAATGTGAATCACTAAACATCTT
  GCATAAATACATCCTTCCAAGCAAAACCGGGCATATCAACCCTCTCGTTGTGTATGGGGGACCATGCACT
  GGGAAGACTCTACTGCTAGCTGAAGTAGCAAAAAAGGCTTATGGCTGGCTACATGAAGACACAGGGCCAG
  ACTCTGACCCAGTTGTCATTGTGAGATTTCTAGGAACAACAGACATGAGTATTGATCTGAGGACACTTCT
  TCTAAGTGTCTGTGAACAGCTGGCCGTCAACTACCGGTGTCTGGTTCAAAGCTTTCCTAAGAAGATCCAC
  GACCTTCGTGACTTGTTTATAAACCTGTTGAATGAGTCTTCACTGCAGAGACCTCTGGTAATCATATTGG
  ATGCCCTAGAGCAGCTCTCGGAGGCCGACGAAGCTAGGAAGCTCTGGTGGCTGCCCGCTCATCTTCCCCG
  GTTTGTGCGGATCATCCTCTCCACACTGCCTAACAAACATGGGATCCTGCAGAAACTCCGGTGCCTTATC
  CATGAAGAAGACAACTACATTGAGCTGATTCCCCGGGACAGAAAGATGTGCAGTCAGGTCCTCAAACATC
  AGCTGCTGAGGGTCAAAAGGAAGGTCACGTCCGGCCAGCAGATTTATGTAAATAATGCATTCTCCAAGTG
  CACACTGCCTATGTTTGTGAACCTAACTTTCAGAGAGGTGAGACACTGGAGATCTCACAAGGATGTCGAT
  GAGTCCTCCCTCTGTGTGACTGTCCACGAAAGCATAGAGCAGTTATTCTGGTCCTTGGAGAAGAAGTGTG
  GTCAGAAACTGGTCTCCAGGGCTCTGGGTTACATCACCATGGCCAAAATGGGTTTGAGTGAAATGGAACT
  GGAAGATGTATTAGCTCTGGACAACAGCGTTATGAATGAACTCAATGAGAACACCAGACCCAGCAATCCC
  CTGAGAGTACCTTACCTGTATATTGCAAGGCTCAAGGAGGGTCTCAATGGGTACTTAATAGAAAGACATG
  TGAAGAATGTCACACTCTTAGTCTGGGCCAACAGACACCTGCAGCTCATAGCTCAGAAGCTGTACCTGCA
  AGAAGACAGCAACCTTCGAGAGATGCACACAATCCTGGCAGATTACTTTCTAGGGGTGTGGTCGGGAGGC
  AGGAGGAAAGCTTTCTGCCTGGAAGACCCCTACTTGAATGGCTGCCTCGACTTAGAGAACAGAAGTCTGC
  TTGAGGAAGAAAAGCACTTCATGGAACAAGCATCCTTCGACAGGCAGGCCCCCGACCAGCCCTGGGTTTT
  CCAGTGTAACCCACTAGAGCCCGACATCTTTTTTGTCAATCACCGGAAGATGTCTGAGCTCTTGTATCAT
  CTGACCAGGTGTGGGAAAACCGATGACCTCCTTTATGGAATCATCATGAACTTCAGCTGGCTTTACACCA
  TGATCAAAATTGGCCAGTTTGACAAAGTGCTTGCCGACATAGAACTGGCTTACAACTACTCGCAGGAAAA
  GGAGCTGAAGTTCCTGGCCAGCACACTCCGTAGCATCAGAAACAAGGTCATTGCATTCCCAGGTTCCCTT
  TCAGCAGAGCTGCAGCAAAGGCTGCTGCCTGTTGTGAGTTCCCTGCCCAAACTCAGACACCTCCTTTTAG
  AATGTGACAAAGACGGACCCAAATACTGCTCCATCGTTCCTCTCCATTCATCCATGGATGTGACATACAG
  CCCCGAGCGCCTCCCCTTAGCATCCAGCCACCTGCACGTCACAGAGATCCTCCCCACCTGTAACCCCAGC
  ACTGTTCTCACAGCTCTGGAAAATGGTTCCATCAGCACCTGGGATGTAGAAACTCGCCAGCTCCTCCGGC
  AAATCACTACAGCCCAGTCCGTTATCCTGGGCATGAAGCTGAGCAGTGATGAGAAGTACCTTGTGGTTGC
  TACGACAAACAACACCTTGCTGATTTACGACAATGTCAATTCCTGTCTCCTGTCTGAGGTGGAAATCAAA
  GGGACCAAGCATGGAAGCGGCTCCACCTACATCAATGGATTTACACTCTCGGTCAATCATGCCCTTGCGT
  GGCTAGAGGCCAGCAAAGATGTCACTGTCATTGACCTGCTCTATGGATGGCCCCTTTACCAGTTCCACTG
  CTGGTATGAGGTGACATGTGTCCAGTGCTCCCTGGATGGGGTATATGCTTTCTGTGGCCAATACCTCAAT
  AACACCACCATTTTCCACTTAGGGAGTGGAGAAAAAATATGTACCGTGACGTCAGAATTTTCAGGTGGCT
  TTGTGAAGTTTCTTCTCATCTTGGACACAGCTCAAGAGATGGTCATGGTAGACAGTGAGGGAAGTCTCTC
  GGTTTGGAACACCGAGGACATATCCAACCCCCAGCTGACTGAAGACTTTGACTGTCGGAAGGAAGACAGC
  GAAGTGGTGAGCATTGAGCTTTCGGAAGACCAAAGTGCGATTCTGATCTGTAAAGCTCTCAGCATTGAGC
  TCTTAGACACCGGCATGTGGAAGGTAGCTGAAAAATTCCGAGCCAGGCACAACGAACGCTTTGTATCTGC
  CGTGCTATCCAAAAACGGGGACTGTATCATCGCAACCATGGAGAATACCCCAGCGGTATTCTTCTGGAGG
  CGGGACACAGGACAGTGCCTGGCGAGCTTGCAGGAAAGCTCGGGTACCATCGTTAAGTTGGTAAAATCCA
  GTCACCACAATATGCTACTGTCTTTGTCAACCAGTGGCGTTCTTTCCATTTGGGATATAGACATAATCAC
  AGCTATGTCCAATATCGATAAAACTGGAAAACCCATTCAGAGTCTGGTGTTGCCGGCCAGGGGGGAGATC
  ATCTACTCTCTCGATGGCTCCGATTGTGTACATAAATGGAACTTCAGCAGTGGGTTCATTGAAGCAGTAT
  TTAAGCATGAAGGGATAGTCGAACACTGTGTGTTAACGTCCACTGGGGACCTAATGGTGACGTCAGATGA
  CAAAAGCAGCCAGTACGTCTGGCATACCAGCAGTGGGGAAAACCTCTTCCGAATTAACGGGCAGAGGATC
  TCTCAGCTGCTGATCACCCACAACGACCAGTTTGTGGTCTCTCTCTGTGAGGAGAACGCCTCCAGGGTTT
  GGAGGCTGGCCACAGGCCACAGAGTCTGCAACATCTTGACCACTTTGCAAAATGCCTTCATTACCTCTGC
  AAACACCTTCGTGGTGGGAATGACGAAAAGCAAAGTGCTGGCAGTCAGTCTCTGGACCGGAAGTATCACC
  AAGAAATTCTGCTGTGAGGATGGGATCACCATTGTGAATTTCAAGTTGATCCCCGACTGTCCTGACGTCA
  TCGTGTTTATCACGTCAGCCGAGACCGTGAACCTCTGGAGCCTGACTGATGAAGTGATTTGTCGGCGTGT
  GCAGCTTCCAAGCAACTTCTTGAAAAACCTGGAGGATTTTGAAATTTCTCCCAATGGGAAGCTCGGCATT
  ATATCCCGTGGAGATGAAAATATCAATGTGCTGGATTTACACAGTGGAAAGCTACGGGTGGTTCACGCCT
  CTGGAGTCATCTGGAGGCAGAGGCTCTCTCGAGATGGTCGCTACCTGGTGTATATTTGTTTCCGGAATGG
  GGAGGAGGAGGAGGAAAACGATGCTATTTCTAGTTTAATAGTGATGAGACTGGCTGACGGCAAAAACATC
  GGTGCTTGCTCCCTATACAAAACACCAACTTTCCTTGCACTTTCTCAGAGACACCTGAACATCATCGTCG
  GCTTTGATGATGGGAGTATAGGGATATACACAGTTGTGGATCGTGTAGATGCTGCGTTGAAGATCAAAAT
  TGCCACATCGAATAGCAGACAGATTTTCAACAATGCAACGCAGACATCCAGGCCAAAGTCAAACAGCTAC
  TCCTTTAAAGTGTCTGTGGATTGCTTATGGCGAGAGTCCACTGAGGTCTTTGCAAGAGACAGCCCCATCA
  CCGTGAGTGACTCTTCTGAGTCCAACGAGGCAACACCCTCCAAGAAACACAATTCTTGTTATGACCGAGT
  GTGTGCTGCCCTAGAATCCAGGAGCCACAGCTACACTCCAGATAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220859 representing NM_177006
  Red=Cloning site Green=Tags(s)
  
  MWPAGAGTKLPCPRDSALRRAAFSGNLTALPSHLVPAGRSVRVFISANPEDTGAERQALRETVYPKLREF
  CRENYGLEFQVIDLYWGIEEDEWDSPELQKMRMKLLEECLKTSAGPCFVGLLGEKYGNIRIPGEVEASEF
  EMILDAAVEAKLETKLLEDWYCRDENSVPAAYYLRPRLEVPRSNKNSTQPSASSEQERPWQEISDEIKTI
  FKAAVKLLHEQGKMKQSQAKRYLFSAIEDEFDFALGKQTPAFLKKCVCYIRKIANIERFVKIPEMGKYMD
  ITGTDPRIVRDPEAQEKLIKLRDEFIPTIVASSNLRVYTSVTHCDMKLGYSQEIENHYIEGLGKQFYEDM
  IDIIQATVQQNFDTETDTLYDEILQHSSLCKTYASFYEYKCESLNILHKYILPSKTGHINPLVVYGGPCT
  GKTLLLAEVAKKAYGWLHEDTGPDSDPVVIVRFLGTTDMSIDLRTLLLSVCEQLAVNYRCLVQSFPKKIH
  DLRDLFINLLNESSLQRPLVIILDALEQLSEADEARKLWWLPAHLPRFVRIILSTLPNKHGILQKLRCLI
  HEEDNYIELIPRDRKMCSQVLKHQLLRVKRKVTSGQQIYVNNAFSKCTLPMFVNLTFREVRHWRSHKDVD
  ESSLCVTVHESIEQLFWSLEKKCGQKLVSRALGYITMAKMGLSEMELEDVLALDNSVMNELNENTRPSNP
  LRVPYLYIARLKEGLNGYLIERHVKNVTLLVWANRHLQLIAQKLYLQEDSNLREMHTILADYFLGVWSGG
  RRKAFCLEDPYLNGCLDLENRSLLEEEKHFMEQASFDRQAPDQPWVFQCNPLEPDIFFVNHRKMSELLYH
  LTRCGKTDDLLYGIIMNFSWLYTMIKIGQFDKVLADIELAYNYSQEKELKFLASTLRSIRNKVIAFPGSL
  SAELQQRLLPVVSSLPKLRHLLLECDKDGPKYCSIVPLHSSMDVTYSPERLPLASSHLHVTEILPTCNPS
  TVLTALENGSISTWDVETRQLLRQITTAQSVILGMKLSSDEKYLVVATTNNTLLIYDNVNSCLLSEVEIK
  GTKHGSGSTYINGFTLSVNHALAWLEASKDVTVIDLLYGWPLYQFHCWYEVTCVQCSLDGVYAFCGQYLN
  NTTIFHLGSGEKICTVTSEFSGGFVKFLLILDTAQEMVMVDSEGSLSVWNTEDISNPQLTEDFDCRKEDS
  EVVSIELSEDQSAILICKALSIELLDTGMWKVAEKFRARHNERFVSAVLSKNGDCIIATMENTPAVFFWR
  RDTGQCLASLQESSGTIVKLVKSSHHNMLLSLSTSGVLSIWDIDIITAMSNIDKTGKPIQSLVLPARGEI
  IYSLDGSDCVHKWNFSSGFIEAVFKHEGIVEHCVLTSTGDLMVTSDDKSSQYVWHTSSGENLFRINGQRI
  SQLLITHNDQFVVSLCEENASRVWRLATGHRVCNILTTLQNAFITSANTFVVGMTKSKVLAVSLWTGSIT
  KKFCCEDGITIVNFKLIPDCPDVIVFITSAETVNLWSLTDEVICRRVQLPSNFLKNLEDFEISPNGKLGI
  ISRGDENINVLDLHSGKLRVVHASGVIWRQRLSRDGRYLVYICFRNGEEEEENDAISSLIVMRLADGKNI
  GACSLYKTPTFLALSQRHLNIIVGFDDGSIGIYTVVDRVDAALKIKIATSNSRQIFNNATQTSRPKSNSY
  SFKVSVDCLWRESTEVFARDSPITVSDSSESNEATPSKKHNSCYDRVCAALESRSHSYTPDN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177006
• ORF Size: 5226 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177006.3, NP_795980.2
• RefSeq Size: 8539 bp
• RefSeq ORF: 5229 bp
• Locus ID: 319807
• UniProt ID: Q6P5U7
• Cytogenetics: 5 C3.1
• MW: 197.4 kDa