Nwd2 (NM_177006) Mouse Untagged Clone

CAT#: MC224896

3110047P20Rik (untagged) - Mouse RIKEN cDNA 3110047P20 gene (3110047P20Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nwd2
• Synonyms: 3110047P20Rik; B830017A01Rik; Hn1-ps2; mKIAA1239
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224896 representing NM_177006
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCCGGCCGGTGCGGGCACCAAGCTGCCCTGTCCCCGGGACTCCGCGCTCCGGCGGGCGGCGTTCT
  CTGGTAATCTCACCGCCCTTCCTTCTCACCTGGTACCCGCCGGTCGCAGCGTCCGGGTCTTCATCAGCGC
  CAACCCTGAAGATACAGGAGCGGAACGGCAGGCACTAAGAGAAACTGTGTATCCGAAACTGAGAGAATTC
  TGCAGAGAAAACTATGGGTTGGAATTTCAGGTCATAGATCTGTACTGGGGAATTGAGGAAGATGAATGGG
  ACAGCCCCGAACTGCAGAAGATGCGCATGAAGCTGCTGGAGGAATGCTTGAAAACTTCTGCAGGTCCATG
  CTTTGTTGGCCTCTTAGGTGAAAAATATGGGAACATTCGAATCCCTGGGGAAGTAGAAGCTTCAGAGTTT
  GAAATGATTTTGGATGCTGCTGTCGAGGCAAAATTAGAGACCAAGTTACTGGAAGATTGGTACTGTCGGG
  ATGAGAACTCTGTGCCAGCAGCCTATTACCTCAGACCCAGGCTGGAGGTGCCAAGAAGCAACAAAAACTC
  AACACAGCCTTCTGCCAGTAGTGAACAGGAGAGGCCATGGCAAGAAATATCGGATGAAATCAAAACGATA
  TTTAAAGCTGCTGTCAAACTGCTGCATGAACAGGGGAAAATGAAGCAAAGTCAGGCTAAGAGGTACCTGT
  TCTCAGCTATCGAGGATGAGTTTGACTTTGCTCTAGGCAAGCAAACTCCAGCCTTCCTGAAGAAGTGTGT
  TTGCTACATCAGAAAGATTGCTAACATCGAACGCTTCGTGAAAATCCCAGAGATGGGGAAATACATGGAC
  ATAACCGGAACAGACCCAAGAATCGTCCGTGACCCAGAAGCTCAAGAGAAGCTGATAAAACTCAGGGATG
  AATTCATTCCTACCATTGTTGCATCCTCTAATTTGAGAGTGTACACATCTGTCACTCACTGTGACATGAA
  ACTGGGCTATTCCCAAGAGATAGAAAACCATTACATAGAAGGACTTGGTAAGCAATTTTATGAAGACATG
  ATTGATATAATTCAGGCAACAGTACAGCAGAACTTTGACACGGAGACTGATACGCTGTACGATGAAATCC
  TGCAGCACTCCTCACTGTGTAAAACATATGCCTCCTTCTATGAATACAAATGTGAATCACTAAACATCTT
  GCATAAATACATCCTTCCAAGCAAAACCGGGCATATCAACCCTCTCGTTGTGTATGGGGGACCATGCACT
  GGGAAGACTCTACTGCTAGCTGAAGTAGCAAAAAAGGCTTATGGCTGGCTACATGAAGACACAGGGCCAG
  ACTCTGACCCAGTTGTCATTGTGAGATTTCTAGGAACAACAGACATGAGTATTGATCTGAGGACACTTCT
  TCTAAGTGTCTGTGAACAGCTGGCCGTCAACTACCGGTGTCTGGTTCAAAGCTTTCCTAAGAAGATCCAC
  GACCTTCGTGACTTGTTTATAAACCTGTTGAATGAGTCTTCACTGCAGAGACCTCTGGTAATCATATTGG
  ATGCCCTAGAGCAGCTCTCGGAGGCCGACGAAGCTAGGAAGCTCTGGTGGCTGCCCGCTCATCTTCCCCG
  GTTTGTGCGGATCATCCTCTCCACACTGCCTAACAAACATGGGATCCTGCAGAAACTCCGGTGCCTTATC
  CATGAAGAAGACAACTACATTGAGCTGATTCCCCGGGACAGAAAGATGTGCAGTCAGGTCCTCAAACATC
  AGCTGCTGAGGGTCAAAAGGAAGGTCACGTCCGGCCAGCAGATTTATGTAAATAATGCATTCTCCAAGTG
  CACACTGCCTATGTTTGTGAACCTAACTTTCAGAGAGGTGAGACACTGGAGATCTCACAAGGATGTCGAT
  GAGTCCTCCCTCTGTGTGACTGTCCACGAAAGCATAGAGCAGTTATTCTGGTCCTTGGAGAAGAAGTGTG
  GTCAGAAACTGGTCTCCAGGGCTCTGGGTTACATCACCATGGCCAAAATGGGTTTGAGTGAAATGGAACT
  GGAAGATGTATTAGCTCTGGACAACAGCGTTATGAATGAACTCAATGAGAACACCAGACCCAGCAATCCC
  CTGAGAGTACCTTACCTGTATATTGCAAGGCTCAAGGAGGGTCTCAATGGGTACTTAATAGAAAGACATG
  TGAAGAATGTCACACTCTTAGTCTGGGCCAACAGACACCTGCAGCTCATAGCTCAGAAGCTGTACCTGCA
  AGAAGACAGCAACCTTCGAGAGATGCACACAATCCTGGCAGATTACTTTCTAGGGGTGTGGTCGGGAGGC
  AGGAGGAAAGCTTTCTGCCTGGAAGACCCCTACTTGAATGGCTGCCTCGACTTAGAGAACAGAAGTCTGC
  TTGAGGAAGAAAAGCACTTCATGGAACAAGCATCCTTCGACAGGCAGGCCCCCGACCAGCCCTGGGTTTT
  CCAGTGTAACCCACTAGAGCCCGACATCTTTTTTGTCAATCACCGGAAGATGTCTGAGCTCTTGTATCAT
  CTGACCAGGTGTGGGAAAACCGATGACCTCCTTTATGGAATCATCATGAACTTCAGCTGGCTTTACACCA
  TGATCAAAATTGGCCAGTTTGACAAAGTGCTTGCCGACATAGAACTGGCTTACAACTACTCGCAGGAAAA
  GGAGCTGAAGTTCCTGGCCAGCACACTCCGTAGCATCAGAAACAAGGTCATTGCATTCCCAGGTTCCCTT
  TCAGCAGAGCTGCAGCAAAGGCTGCTGCCTGTTGTGAGTTCCCTGCCCAAACTCAGACACCTCCTTTTAG
  AATGTGACAAAGACGGACCCAAATACTGCTCCATCGTTCCTCTCCATTCATCCATGGATGTGACATACAG
  CCCCGAGCGCCTCCCCTTAGCATCCAGCCACCTGCACGTCACAGAGATCCTCCCCACCTGTAACCCCAGC
  ACTGTTCTCACAGCTCTGGAAAATGGTTCCATCAGCACCTGGGATGTAGAAACTCGCCAGCTCCTCCGGC
  AAATCACTACAGCCCAGTCCGTTATCCTGGGCATGAAGCTGAGCAGTGATGAGAAGTACCTTGTGGTTGC
  TACGACAAACAACACCTTGCTGATTTACGACAATGTCAATTCCTGTCTCCTGTCTGAGGTGGAAATCAAA
  GGGACCAAGCATGGAAGCGGCTCCACCTACATCAATGGATTTACACTCTCGGTCAATCATGCCCTTGCGT
  GGCTAGAGGCCAGCAAAGATGTCACTGTCATTGACCTGCTCTATGGATGGCCCCTTTACCAGTTCCACTG
  CTGGTATGAGGTGACATGTGTCCAGTGCTCCCTGGATGGGGTATATGCTTTCTGTGGCCAATACCTCAAT
  AACACCACCATTTTCCACTTAGGGAGTGGAGAAAAAATATGTACCGTGACGTCAGAATTTTCAGGTGGCT
  TTGTGAAGTTTCTTCTCATCTTGGACACAGCTCAAGAGATGGTCATGGTAGACAGTGAGGGAAGTCTCTC
  GGTTTGGAACACCGAGGACATATCCAACCCCCAGCTGACTGAAGACTTTGACTGTCGGAAGGAAGACAGC
  GAAGTGGTGAGCATTGAGCTTTCGGAAGACCAAAGTGCGATTCTGATCTGTAAAGCTCTCAGCATTGAGC
  TCTTAGACACCGGCATGTGGAAGGTAGCTGAAAAATTCCGAGCCAGGCACAACGAACGCTTTGTATCTGC
  CGTGCTATCCAAAAACGGGGACTGTATCATCGCAACCATGGAGAATACCCCAGCGGTATTCTTCTGGAGG
  CGGGACACAGGACAGTGCCTGGCGAGCTTGCAGGAAAGCTCGGGTACCATCGTTAAGTTGGTAAAATCCA
  GTCACCACAATATGCTACTGTCTTTGTCAACCAGTGGCGTTCTTTCCATTTGGGATATAGACATAATCAC
  AGCTATGTCCAATATCGATAAAACTGGAAAACCCATTCAGAGTCTGGTGTTGCCGGCCAGGGGGGAGATC
  ATCTACTCTCTCGATGGCTCCGATTGTGTACATAAATGGAACTTCAGCAGTGGGTTCATTGAAGCAGTAT
  TTAAGCATGAAGGGATAGTCGAACACTGTGTGTTAACGTCCACTGGGGACCTAATGGTGACGTCAGATGA
  CAAAAGCAGCCAGTACGTCTGGCATACCAGCAGTGGGGAAAACCTCTTCCGAATTAACGGGCAGAGGATC
  TCTCAGCTGCTGATCACCCACAACGACCAGTTTGTGGTCTCTCTCTGTGAGGAGAACGCCTCCAGGGTTT
  GGAGGCTGGCCACAGGCCACAGAGTCTGCAACATCTTGACCACTTTGCAAAATGCCTTCATTACCTCTGC
  AAACACCTTCGTGGTGGGAATGACGAAAAGCAAAGTGCTGGCAGTCAGTCTCTGGACCGGAAGTATCACC
  AAGAAATTCTGCTGTGAGGATGGGATCACCATTGTGAATTTCAAGTTGATCCCCGACTGTCCTGACGTCA
  TCGTGTTTATCACGTCAGCCGAGACCGTGAACCTCTGGAGCCTGACTGATGAAGTGATTTGTCGGCGTGT
  GCAGCTTCCAAGCAACTTCTTGAAAAACCTGGAGGATTTTGAAATTTCTCCCAATGGGAAGCTCGGCATT
  ATATCCCGTGGAGATGAAAATATCAATGTGCTGGATTTACACAGTGGAAAGCTACGGGTGGTTCACGCCT
  CTGGAGTCATCTGGAGGCAGAGGCTCTCTCGAGATGGTCGCTACCTGGTGTATATTTGTTTCCGGAATGG
  GGAGGAGGAGGAGGAAAACGATGCTATTTCTAGTTTAATAGTGATGAGACTGGCTGACGGCAAAAACATC
  GGTGCTTGCTCCCTATACAAAACACCAACTTTCCTTGCACTTTCTCAGAGACACCTGAACATCATCGTCG
  GCTTTGATGATGGGAGTATAGGGATATACACAGTTGTGGATCGTGTAGATGCTGCGTTGAAGATCAAAAT
  TGCCACATCGAATAGCAGACAGATTTTCAACAATGCAACGCAGACATCCAGGCCAAAGTCAAACAGCTAC
  TCCTTTAAAGTGTCTGTGGATTGCTTATGGCGAGAGTCCACTGAGGTCTTTGCAAGAGACAGCCCCATCA
  CCGTGAGTGACTCTTCTGAGTCCAACGAGGCAACACCCTCCAAGAAACACAATTCTTGTTATGACCGAGT
  GTGTGCTGCCCTAGAATCCAGGAGCCACAGCTACACTCCAGATAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177006
• Insert Size: 5229 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177006.3, NP_795980.2
• RefSeq Size: 8539 bp
• RefSeq ORF: 5229 bp
• Locus ID: 319807
• UniProt ID: Q6P5U7
• Cytogenetics: 5 C3.1