Slc4a4 (NM_001136260) Mouse Tagged ORF Clone

CAT#: MR220828

Slc4a4 (Myc-DDK-tagged) - Mouse solute carrier family 4 (anion exchanger), member 4 (Slc4a4), transcript variant 2

Product Images

Circular map for MR220828

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc4a4
• Synonyms: AI835705; NBC; NBC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220828 representing NM_001136260
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATGAAGCTGTCCTGGACAGAGGGGCTTCCTTCCTTAAACATGTGTGTGATGAAGAAGAAGTAG
  AAGGTCACCACACGATCTACATTGGGGTCCATGTGCCCAAGAGCTACCGGAGAAGGAGACGCCACAAGAG
  GAAGGCTGGGCACAAGGAAAAGAAGGAAAAGGAGAGAATCTCCGAGAACTACTCCGACAAATCTGATGTG
  GAGAATGCGGATGAGTCCAGCAGCAGTATCCTCAAACCCCTCATCTCCCCGGCCGCAGAACGCATCCGAT
  TCATCTTGGGAGAGGAAGATGACAGCCCGGCACCTCCTCAGCTCTTCACGGAACTCGATGAGCTTCTGGC
  TGTGGATGGACAGGAGATGGAATGGAAGGAGACAGCGAGGTGGATTAAGTTTGAAGAGAAAGTGGAGCAG
  GGTGGGGAGCGATGGAGCAAACCCCATGTGGCCACCTTGTCCCTGCACAGCCTGTTTGAGCTGAGGACAT
  GTATGGAGAAAGGATCCATCATGCTTGACCGGGAGGCATCTTCTCTCCCACAGCTGGTGGAGATGATTGC
  AGACCACCAGATCGAGACAGGCCTACTGAAGCCTGACCTGAAGGATAAGGTCACCTATACTCTGCTCCGG
  AAACATCGACATCAAACCAAGAAATCCAACCTTCGGTCCCTGGCTGACATTGGGAAGACTGTCTCCAGTG
  CAAGTAGGATGTTTAGCAACCCTGATAATGGCAGCCCAGCCATGACCCACAGGAATCTGACATCCTCCAG
  TCTCAATGACATTTCTGATAAACCAGAGAAGGATCAGCTGAAGAATAAATTCATGAAAAAACTGCCCCGA
  GATGCGGAAGCTTCCAATGTGCTTGTTGGGGAGGTTGACTTCTTGGACACTCCCTTCATTGCCTTTGTTC
  GCCTACAGCAGGCTGTCATGCTGGGTGCCCTGACTGAGGTCCCTGTGCCCACAAGGTTCTTGTTCATTCT
  CTTAGGTCCAAAGGGGAAAGCCAAGTCCTACCATGAGATTGGAAGAGCTATCGCCACCTTGATGTCTGAC
  GAGGTGTTCCACGACATCGCTTACAAAGCGAAAGACAGACACGACCTGATTGCTGGCATTGATGAGTTCT
  TAGATGAAGTCATTGTCCTTCCACCTGGGGAATGGGACCCCACAATCCGAATAGAGCCTCCAAAGAGTCT
  CCCATCATCTGACAAAAGAAAGAATATGTACTCAGGTGGAGAGAATGTTCAGATGAATGGGGACACACCT
  CATGATGGAGGCCACGGAGGAGGAGGACATGGTGACTGTGAAGAACTACAGAGAACTGGCCGGTTCTGCG
  GTGGATTAATTAAGGACATAAAGAGGAAAGCACCATTTTTTGCCAGTGACTTTTATGATGCTTTAAACAT
  TCAGGCTCTCTCTGCGATTCTCTTCATTTATCTGGCAACCGTAACCAACGCCATCACTTTTGGAGGCCTG
  CTCGGGGATGCCACCGACAACATGCAGGGTGTGTTGGAGAGTTTCCTGGGCACTGCTGTCTCCGGAGCCA
  TCTTCTGCCTTTTTGCGGGTCAACCGCTTACCATCTTGAGCAGCACGGGACCAGTGTTGGTGTTTGAGAG
  GCTTCTTTTTAACTTCAGCAAGGACCATAATTTTGACTACTTGGAGTTTCGTCTTTGGATTGGCCTGTGG
  TCAGCCTTCATGTGTCTTGTCCTGGTGGCCACTGATGCGAGCTTCCTGGTTCAGTACTTCACCCGTTTCA
  CAGAAGAAGGTTTCTCCTCTCTCATCAGCTTCATCTTCATCTATGATGCTTTCAAGAAGATGATCAAGCT
  AGCAGATTACTATCCCATCAACTCTGACTTCAAAGTGGGTTACAATACTCACTTCTCTTGTGCTTGCCTG
  CCACCCGACCCAGTTAATCTCTCAGTATCTAATGATACCACACTAGCCCCAGAGGACCTGCCGACCATTT
  CTTCTACTGACATGTACCATAATGTCACCTTTGACTGGGCCTATTTGTCAAAGAAGGAGTGTGTGAAGTA
  TGGAGGGAAGCTCGTGGGAAACAACTGTGACTTCGTGCCTGATATCACACTCATGTCCTTCATTCTCTTC
  CTGGGCACTTACACCTCGTCTATGGCTATGAAGAAATTCAAAACCAGTCGCTATTTTCCAACCACAGCAA
  GAAAACTGATCAGTGATTTTGCCATTATCCTGTCCATTCTCATATTCTGTGTAATAGATGCCCTAGTCGG
  CGTGGACACTCCGAAGCTCATTGTACCAAGTGAGTTCAAGCCAACAAGTCCTAACAGGGGTTGGTTTGTC
  CCGCCATTTGGAGGAAACCCTTGGTGGGTGTGCCTTGCTGCTGCCATCCCGGCTTTGCTAGTCACCATCC
  TGATTTTCATGGACCAGCAGATCACCGCTGTGATTGTGAACAGGAAAGAGCATAAACTCAAGAAAGGAGC
  TGGGTATCACCTGGATCTGTTTTGGGTCGCCATCCTCATGGTGGTATGCTCCTTCATGGCTCTTCCCTGG
  TATGTGGCTGCTACTGTTATCTCCATTGCCCACATTGACAGTCTGAAGATGGAGACGGAGACATCTGCGC
  CTGGAGAACAACCAAAATTTCTGGGAGTAAGGGAACAACGAGTCACTGGAACCCTTGTGTTTATTCTGAC
  TGGCCTGTCAGTCTTCATGGCTCCCATCCTGAAGTTTATCCCGATGCCTGTGCTGTATGGTGTGTTCCTG
  TATATGGGGGTGGCCTCACTTAACGGTGTGCAGTTCATGGACCGTCTCAAGCTGCTGCTGATGCCCTTGA
  AGCATCAGCCCGACTTCATCTACCTGCGCCACGTCCCCCTCCGCCGAGTCCACCTGTTCACCTTCCTGCA
  GGTGTTGTGCCTGGCTCTGCTCTGGATCCTCAAGTCAACAGTGGCTGCAATTATTTTTCCTGTTATGATC
  CTGGCCCTGGTAGCAGTCAGAAAAGGTATGGATTACCTCTTCTCCCAGCACGACCTCAGCTTCCTTGATG
  ATGTCATTCCAGAAAAGGACAAGAAAAAGAAGGAGGACGAGAAGAAAAAGAAAAAGAAGAAAGGAAGTTT
  GGATAGCGACAATGACGATGAGAAAGATCCTCAACATTCCTCGAACGCCACACATCATGCTGATAAAATT
  CCTTTCCTTGAGTCGCTGGGTTTACCCAGTCCTCCAAGAACTCCAGTGAAAGTCGTGCCTCAAATTAGAA
  TCGAACGTGAGCCTGAAGACAACGATTATCTTTGGAGGAGCAAGGGAACAGAAACCACGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220828 representing NM_001136260
  Red=Cloning site Green=Tags(s)
  
  MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKAGHKEKKEKERISENYSDKSDV
  ENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQ
  GGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIADHQIETGLLKPDLKDKVTYTLLR
  KHRHQTKKSNLRSLADIGKTVSSASRMFSNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPR
  DAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSD
  EVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPTIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTP
  HDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGL
  LGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDHNFDYLEFRLWIGLW
  SAFMCLVLVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSDFKVGYNTHFSCACL
  PPDPVNLSVSNDTTLAPEDLPTISSTDMYHNVTFDWAYLSKKECVKYGGKLVGNNCDFVPDITLMSFILF
  LGTYTSSMAMKKFKTSRYFPTTARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPNRGWFV
  PPFGGNPWWVCLAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVVCSFMALPW
  YVAATVISIAHIDSLKMETETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIPMPVLYGVFL
  YMGVASLNGVQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTFLQVLCLALLWILKSTVAAIIFPVMI
  LALVAVRKGMDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSDNDDEKDPQHSSNATHHADKI
  PFLESLGLPSPPRTPVKVVPQIRIEREPEDNDYLWRSKGTETTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136260
• ORF Size: 3282 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136260.1, NP_001129732.1
• RefSeq Size: 7395 bp
• RefSeq ORF: 3285 bp
• Locus ID: 54403
• UniProt ID: O88343
• Cytogenetics: 5 E1
• MW: 123.5 kDa
• Gene Summary: Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.[UniProtKB/Swiss-Prot Function]