Slc4a4 (NM_001136260) Mouse Untagged Clone

CAT#: MC223511

Slc4a4 (untagged) - Mouse solute carrier family 4 (anion exchanger), member 4 (Slc4a4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc4a4
• Synonyms: AI835705; NBC; NBC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223511 representing NM_001136260
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATGAAGCTGTCCTGGACAGAGGGGCTTCCTTCCTTAAACATGTGTGTGATGAAGAAGAAGTAG
  AAGGTCACCACACGATCTACATTGGGGTCCATGTGCCCAAGAGCTACCGGAGAAGGAGACGCCACAAGAG
  GAAGGCTGGGCACAAGGAAAAGAAGGAAAAGGAGAGAATCTCCGAGAACTACTCCGACAAATCTGATGTG
  GAGAATGCGGATGAGTCCAGCAGCAGTATCCTCAAACCCCTCATCTCCCCGGCCGCAGAACGCATCCGAT
  TCATCTTGGGAGAGGAAGATGACAGCCCGGCACCTCCTCAGCTCTTCACGGAACTCGATGAGCTTCTGGC
  TGTGGATGGACAGGAGATGGAATGGAAGGAGACAGCGAGGTGGATTAAGTTTGAAGAGAAAGTGGAGCAG
  GGTGGGGAGCGATGGAGCAAACCCCATGTGGCCACCTTGTCCCTGCACAGCCTGTTTGAGCTGAGGACAT
  GTATGGAGAAAGGATCCATCATGCTTGACCGGGAGGCATCTTCTCTCCCACAGCTGGTGGAGATGATTGC
  AGACCACCAGATCGAGACAGGCCTACTGAAGCCTGACCTGAAGGATAAGGTCACCTATACTCTGCTCCGG
  AAACATCGACATCAAACCAAGAAATCCAACCTTCGGTCCCTGGCTGACATTGGGAAGACTGTCTCCAGTG
  CAAGTAGGATGTTTAGCAACCCTGATAATGGCAGCCCAGCCATGACCCACAGGAATCTGACATCCTCCAG
  TCTCAATGACATTTCTGATAAACCAGAGAAGGATCAGCTGAAGAATAAATTCATGAAAAAACTGCCCCGA
  GATGCGGAAGCTTCCAATGTGCTTGTTGGGGAGGTTGACTTCTTGGACACTCCCTTCATTGCCTTTGTTC
  GCCTACAGCAGGCTGTCATGCTGGGTGCCCTGACTGAGGTCCCTGTGCCCACAAGGTTCTTGTTCATTCT
  CTTAGGTCCAAAGGGGAAAGCCAAGTCCTACCATGAGATTGGAAGAGCTATCGCCACCTTGATGTCTGAC
  GAGGTGTTCCACGACATCGCTTACAAAGCGAAAGACAGACACGACCTGATTGCTGGCATTGATGAGTTCT
  TAGATGAAGTCATTGTCCTTCCACCTGGGGAATGGGACCCCACAATCCGAATAGAGCCTCCAAAGAGTCT
  CCCATCATCTGACAAAAGAAAGAATATGTACTCAGGTGGAGAGAATGTTCAGATGAATGGGGACACACCT
  CATGATGGAGGCCACGGAGGAGGAGGACATGGTGACTGTGAAGAACTACAGAGAACTGGCCGGTTCTGCG
  GTGGATTAATTAAGGACATAAAGAGGAAAGCACCATTTTTTGCCAGTGACTTTTATGATGCTTTAAACAT
  TCAGGCTCTCTCTGCGATTCTCTTCATTTATCTGGCAACCGTAACCAACGCCATCACTTTTGGAGGCCTG
  CTCGGGGATGCCACCGACAACATGCAGGGTGTGTTGGAGAGTTTCCTGGGCACTGCTGTCTCCGGAGCCA
  TCTTCTGCCTTTTTGCGGGTCAACCGCTTACCATCTTGAGCAGCACGGGACCAGTGTTGGTGTTTGAGAG
  GCTTCTTTTTAACTTCAGCAAGGACCATAATTTTGACTACTTGGAGTTTCGTCTTTGGATTGGCCTGTGG
  TCAGCCTTCATGTGTCTTGTCCTGGTGGCCACTGATGCGAGCTTCCTGGTTCAGTACTTCACCCGTTTCA
  CAGAAGAAGGTTTCTCCTCTCTCATCAGCTTCATCTTCATCTATGATGCTTTCAAGAAGATGATCAAGCT
  AGCAGATTACTATCCCATCAACTCTGACTTCAAAGTGGGTTACAATACTCACTTCTCTTGTGCTTGCCTG
  CCACCCGACCCAGTTAATCTCTCAGTATCTAATGATACCACACTAGCCCCAGAGGACCTGCCGACCATTT
  CTTCTACTGACATGTACCATAATGTCACCTTTGACTGGGCCTATTTGTCAAAGAAGGAGTGTGTGAAGTA
  TGGAGGGAAGCTCGTGGGAAACAACTGTGACTTCGTGCCTGATATCACACTCATGTCCTTCATTCTCTTC
  CTGGGCACTTACACCTCGTCTATGGCTATGAAGAAATTCAAAACCAGTCGCTATTTTCCAACCACAGCAA
  GAAAACTGATCAGTGATTTTGCCATTATCCTGTCCATTCTCATATTCTGTGTAATAGATGCCCTAGTCGG
  CGTGGACACTCCGAAGCTCATTGTACCAAGTGAGTTCAAGCCAACAAGTCCTAACAGGGGTTGGTTTGTC
  CCGCCATTTGGAGGAAACCCTTGGTGGGTGTGCCTTGCTGCTGCCATCCCGGCTTTGCTAGTCACCATCC
  TGATTTTCATGGACCAGCAGATCACCGCTGTGATTGTGAACAGGAAAGAGCATAAACTCAAGAAAGGAGC
  TGGGTATCACCTGGATCTGTTTTGGGTCGCCATCCTCATGGTGGTATGCTCCTTCATGGCTCTTCCCTGG
  TATGTGGCTGCTACTGTTATCTCCATTGCCCACATTGACAGTCTGAAGATGGAGACGGAGACATCTGCGC
  CTGGAGAACAACCAAAATTTCTGGGAGTAAGGGAACAACGAGTCACTGGAACCCTTGTGTTTATTCTGAC
  TGGCCTGTCAGTCTTCATGGCTCCCATCCTGAAGTTTATCCCGATGCCTGTGCTGTATGGTGTGTTCCTG
  TATATGGGGGTGGCCTCACTTAACGGTGTGCAGTTCATGGACCGTCTCAAGCTGCTGCTGATGCCCTTGA
  AGCATCAGCCCGACTTCATCTACCTGCGCCACGTCCCCCTCCGCCGAGTCCACCTGTTCACCTTCCTGCA
  GGTGTTGTGCCTGGCTCTGCTCTGGATCCTCAAGTCAACAGTGGCTGCAATTATTTTTCCTGTTATGATC
  CTGGCCCTGGTAGCAGTCAGAAAAGGTATGGATTACCTCTTCTCCCAGCACGACCTCAGCTTCCTTGATG
  ATGTCATTCCAGAAAAGGACAAGAAAAAGAAGGAGGACGAGAAGAAAAAGAAAAAGAAGAAAGGAAGTTT
  GGATAGCGACAATGACGATGAGAAAGATCCTCAACATTCCTCGAACGCCACACATCATGCTGATAAAATT
  CCTTTCCTTGAGTCGCTGGGTTTACCCAGTCCTCCAAGAACTCCAGTGAAAGTCGTGCCTCAAATTAGAA
  TCGAACGTGAGCCTGAAGACAACGATTATCTTTGGAGGAGCAAGGGAACAGAAACCACGTTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001136260
• Insert Size: 3285 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136260.1, NP_001129732.1
• RefSeq Size: 7395 bp
• RefSeq ORF: 3285 bp
• Locus ID: 54403
• UniProt ID: O88343
• Cytogenetics: 5 E1
• Gene Summary: Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon and another exon in the 3' end compared to variant 4, that causes a frameshift. The resulting isoform (b) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform d.