Tnfaip2 (NM_009396) Mouse Tagged ORF Clone

CAT#: MR220784

Tnfaip2 (Myc-DDK-tagged) - Mouse tumor necrosis factor, alpha-induced protein 2 (Tnfaip2)

Product Images

Circular map for MR220784

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnfaip2
• Synonyms: B94; Exoc3l3; M-sec; tnfb94; Tnfip2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220784 representing NM_009396
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGATGGTGACTTTCTTCCAAGGGTTTCCTGGCCAGCAGTCTGTGCCAGGGACCCTGAACTTCG
  CTGTAAGCCCCCAAAAGCCACGCTCTACATCCGAGGCTGAATCAGAGACCTCCATGTCTGAGGCGTCCTC
  TGAGGACCTGATGCCATCCCCGGAGGCTCCCGATGGGGAGGAGGAGTCTGCGAAGAAGAAGGAGAAGAAG
  TCCAAAGGACTGGCCAACATGTTCAGTGTCTTCACCAAAGGGAAAAAGAAAAAAAAGGACCAGCCCAGAT
  TATCAGATCTGGAAGTGCAGCCCAAGCCCAGGCCCGAGTTAGATGGTCCACTGCCCACAGTGGAGGAGCT
  CAAGGAGGCCCTGGAGCACGGGCGGCTGGAGGTGGCCTGGCAGGTGCTGGCGTTGGAGAGGCAGCTCGAG
  GCTGCGGCAGCGGCGGGTGGCATGAGCAACGAGGAGCTGGTGTGGCGGCAGAGCAAGGTGGAGGCTCTGT
  ACGTGCTGCTTTGCGACCAGGTGCTCGGGGTACTGCGGCGGCCGCTGGAGGCGGCGCCCGAGCGGCTGAG
  CCAGGCGCTAGCCGTGGTGTCGCAGGAGGAGCTCGAGGACCGGCGGGCGTCCGGGGGACCCTTGGCGGCG
  GCACTGGAGGCCACGCGCCCGCGGCGATGGCTGCAGCGGTGGAGGGGCGTCGTGGCGGAGGTGGCAGCGG
  AACGTCTGGACGCGCAGCCGGCCACAGCGCCCGAGGGCCGCTCGGAGGCGGAGAGCAGGTTCCTGCACAT
  GGGTCGCACCATGAAGGAGGACCTGGAGGTGGTGGTGGAGAGGCTGAAGCCGCTGTTCCCCGACGAGTTC
  AACGTCGTGCGCACCTACGCCGAAAGCTACCACTACCACTTCGCCAGCCACCTGTGCGCCTTGGCGCAGT
  TCGAGTTGTGTGAAAGGGATACCTACTTGCTGCTGCTCTGGGTGCAGAACCTCTACCCCAATGATATTCT
  CAACAGCCCGAAGTTGGCACAGGAGCTTCAAGGTGTCGGGCTTGGGAGCCTCCTGCCCCCAAAGCAGATC
  AGATTGCTGGAGGCCATGTTCTTGTCCAATGAGGTGACCAGCGTGAAGCAGCTCATGGCCCGAGCTTTAG
  AGCTGGAGTCTCAGCGCTGGACCCAGGATGTGGCTCCCCAGAGCCTGGATGGCCACTGTCACAGTGAGCT
  GGCCATCGACATTCTCCAGATCATTTCACAAGGCCAGACTAAGGCTGAGAACATCACTTCGGACGTGGGG
  ATGCAGATAAAGCAGTTGCTGCTGGTGGAGCTGGCTGCACTACTGAGGAGCTACCAGCGCGCCTTTGATG
  AGTTTCTAGAGAAGAGCAAACTCCTGAGGAATTACAGAGTCAACATCATGGCCAACATCAACAACTGCCT
  GTTCTTCTGGACTTCCGTGGAGCAGAAATGGCAGATATCTCATGATTCTCTGAACCGCCTGCTGGAGCCT
  TTGAAAGACCTCAAGGCCCATGGCTTCGACACCCTGCTCCAGAGCCTATTTTTGGACCTGAAGCCGCTGT
  TTAAGAAGTTCACACAGACCCGTTGGGCAAATCCAGTTGAGACCCTGGAGGAAATCATCACTACTGTGAG
  CAGCAGCCTGCCTGAATTCTCAGAGCTGCAGGACTGTTTCCGGGAGGAGCTCATGGAGACTGTGCACCTG
  CACCTAGTGAAAGAATACATCATCAGGCTCTGCAAACGGCGCCTGGTCCTCAAGACGGCCGAGCAGCAGC
  AGCAGCTGGCAAGGCACATCCTGGCCAACGCGGATGCCATTCAGGGGTTCTGCACTGAGAATGGCTCCAC
  TGCAACCTGGCTGCACCGCGCCCTCCCTATGATTGCTGAGATCATTCGCCTGCAAGATTCCAGTGCCATT
  AAGATCGAGGTGGCCACATACGCCACTTGGTACCCTGACTTCAGCAAAGGCCACCTGAACGCCATCCTGG
  CCATCAAGGGCAATCTACCAAGCAGTGAAGTCAGGAGCATCCGGAACATACTGGACATCAACACGGGAGT
  GCAGGAGCCTCCCAGGCCCCTGTTCTCCCTTATAAAGGTTACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220784 representing NM_009396
  Red=Cloning site Green=Tags(s)
  
  MLKMVTFFQGFPGQQSVPGTLNFAVSPQKPRSTSEAESETSMSEASSEDLMPSPEAPDGEEESAKKKEKK
  SKGLANMFSVFTKGKKKKKDQPRLSDLEVQPKPRPELDGPLPTVEELKEALEHGRLEVAWQVLALERQLE
  AAAAAGGMSNEELVWRQSKVEALYVLLCDQVLGVLRRPLEAAPERLSQALAVVSQEELEDRRASGGPLAA
  ALEATRPRRWLQRWRGVVAEVAAERLDAQPATAPEGRSEAESRFLHMGRTMKEDLEVVVERLKPLFPDEF
  NVVRTYAESYHYHFASHLCALAQFELCERDTYLLLLWVQNLYPNDILNSPKLAQELQGVGLGSLLPPKQI
  RLLEAMFLSNEVTSVKQLMARALELESQRWTQDVAPQSLDGHCHSELAIDILQIISQGQTKAENITSDVG
  MQIKQLLLVELAALLRSYQRAFDEFLEKSKLLRNYRVNIMANINNCLFFWTSVEQKWQISHDSLNRLLEP
  LKDLKAHGFDTLLQSLFLDLKPLFKKFTQTRWANPVETLEEIITTVSSSLPEFSELQDCFREELMETVHL
  HLVKEYIIRLCKRRLVLKTAEQQQQLARHILANADAIQGFCTENGSTATWLHRALPMIAEIIRLQDSSAI
  KIEVATYATWYPDFSKGHLNAILAIKGNLPSSEVRSIRNILDINTGVQEPPRPLFSLIKVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009396
• ORF Size: 2073 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009396.2, NP_033422.2
• RefSeq Size: 3590 bp
• RefSeq ORF: 2076 bp
• Locus ID: 21928
• UniProt ID: Q61333
• Cytogenetics: 12 60.99 cM
• MW: 78.1 kDa
• Gene Summary: May play a role as a mediator of inflammation and angiogenesis.[UniProtKB/Swiss-Prot Function]