Lrrc25 (NM_153074) Mouse Tagged ORF Clone

CAT#: MR220729

Lrrc25 (Myc-DDK-tagged) - Mouse leucine rich repeat containing 25 (Lrrc25)

Product Images

Circular map for MR220729

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrc25
• Synonyms: Mapa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220729 representing NM_153074
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGCATCAGAACTAGGTTGCTGTGGTTATGTCTCCTGATGCTGTTGGCCCTGCTTCACAAGTCAG
  GAAGTCAAGATCTCACCTGCATGGTTCACCCGAGCAGGGTAGACTGGACTCAGACATTTAATGGCACCTG
  CCTCAATTTCAGTGGCCTTGGCCTGTCCCTGCCAAGGAGCCCCTTGCAGGCCAGCCATGCTCAAGTCCTG
  GACCTGTCTAAGAATGGCCTGCAGGTGCTCCCTGGGGCTTTCTTCGACAAGCTGGAAAAGCTGCAGACCC
  TGATTGTCACCCACAACCAGCTGGACAGTGTGGACAGGTCCCTGGCCTTGCGCTGTGACCTGGAGCTCAA
  GGCAGACTGCAGCTGTGGGCTGGCCTCCTGGTATGCTCTCCGCCAGAACTGCTCCGGGCAGCAGCAGCTA
  CTGTGTCTACACCCAGCCACCGAAGCTCCAAGGAACCTCTCCACCTTCCTTCAGGTCAGCTGTCCCCCCA
  GCTGGGGCCCGGGGACCATTGGAGCCCTTGTTGCTGGGACTATCTCCCTGGCTGTGGCTGTCAGTGGATC
  TGTGCTGGCCTGGAGACTTCTTCGCCGCCGCCGCAGAGCCAGTGAGCACAGCCTCAGCAAAGCCCAGATG
  TCCCCACACGATATCCCCAAACCAGTGACAGATTTCCTGCCAAGGTACAGCAGCCGGCGACCTGGCCCCA
  AGGCCCCAGACTCACCACCCAGCAGGTTCACAATGGATTATGAGAATGTCTTTATTGGCCAGCCGGCCGA
  GGACTGCTCATGGTCTGCAGCCAGAAACAGCCCTTCTGGGGACAGTGACTGCTACATGAACTACAGGAGT
  GTCGACCAGGACTCTCAGCCCGTCTATTGCAACCTGGAGTCCCTGGGGCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220729 representing NM_153074
  Red=Cloning site Green=Tags(s)
  
  MGSIRTRLLWLCLLMLLALLHKSGSQDLTCMVHPSRVDWTQTFNGTCLNFSGLGLSLPRSPLQASHAQVL
  DLSKNGLQVLPGAFFDKLEKLQTLIVTHNQLDSVDRSLALRCDLELKADCSCGLASWYALRQNCSGQQQL
  LCLHPATEAPRNLSTFLQVSCPPSWGPGTIGALVAGTISLAVAVSGSVLAWRLLRRRRRASEHSLSKAQM
  SPHDIPKPVTDFLPRYSSRRPGPKAPDSPPSRFTMDYENVFIGQPAEDCSWSAARNSPSGDSDCYMNYRS
  VDQDSQPVYCNLESLGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153074
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153074.3, NP_694714.1
• RefSeq Size: 1273 bp
• RefSeq ORF: 894 bp
• Locus ID: 211228
• UniProt ID: Q8K1T1
• Cytogenetics: 8 B3.3
• MW: 33.1 kDa
• Gene Summary: Plays a role in the inhibition of RLR-mediated type I interferon signaling pathway by targeting DDX58/RIG-I for autophagic degradation. Interacts specifically with ISG15-associated DDX58 to promote interaction between DDX58 and the autophagic cargo receptor p62/SQSTM1 to mediate DDX58 degradation via selective autophagy. Plays also a role in the inhibition of NF-kappa-B signaling pathway and inflammatory response by promoting the degradation of p65/RELA.[UniProtKB/Swiss-Prot Function]