Bace2 (NM_019517) Mouse Tagged ORF Clone

CAT#: MR220631

Bace2 (Myc-DDK-tagged) - Mouse beta-site APP-cleaving enzyme 2 (Bace2)

Product Images

Circular map for MR220631

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bace2
• Synonyms: 1110059C24Rik; A; AEPLC; AI850424; AL; ALP56; ARP1; AS; ASP1; ASP21; BAE; BAE2; CDA1; CDA13; CEA; CEAP1; DRAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220631 representing NM_019517
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCGCTGCTTCGAGCACTCTTGCTCCCGGTGCTGGCGCAGTGGCTCTTGAGTGCGGTCCCCGCGC
  TGGCCCCCGCGCCCTTCACGCTGCCCCTCCAAGTGGCCCGGGCCACGAACCACAGAGCCTCGGCTGTTCC
  CGGACTCGGGACCCCCGAGTTGCCCCGGGCCGATGGTCTGGCCCTCGCACTGGAGCCTGTCAGGGCTACT
  GCCAACTTCTTGGCTATGGTGGACAACCTTCAGGGGGACTCTGGCCGCGGCTACTACCTAGAGATGCTGA
  TCGGGACCCCTCCGCAGAAGGTACAGATTCTTGTGGACACTGGAAGCAGTAACTTCGCTGTGGCAGGTGC
  CCCACACTCCTACATAGACACCTACTTTGACTCAGAGAGCTCCAGCACATACCACTCCAAGGGCTTTGAT
  GTCACTGTGAAGTACACACAGGGAAGCTGGACTGGCTTTGTTGGTGAGGACCTTGTCACCATCCCAAAAG
  GCTTCAACAGCTCTTTCTTGGTCAATATTGCCACTATTTTCGAGTCTGAGAATTTCTTTTTGCCTGGTAT
  TAAATGGAATGGAATCCTTGGACTTGCTTATGCTGCTTTGGCCAAGCCATCAAGCTCTCTGGAGACATTT
  TTTGATTCCCTGGTGGCCCAAGCAAAGATTCCAGACATTTTCTCCATGCAGATGTGCGGGGCTGGATTGC
  CAGTAGCTGGTTCTGGTACCAACGGAGGTAGTCTTGTCCTGGGTGGGATTGAACCAAGTTTGTATAAAGG
  AGATATCTGGTATACCCCAATTAAAGAGGAATGGTACTATCAAATAGAAATCCTGAAGTTGGAAATTGGA
  GGCCAGAACCTCAACCTGGACTGCAGAGAGTATAACGCAGACAAGGCCATTGTGGACAGTGGCACCACGC
  TCCTGCGCCTGCCCCAGAAGGTGTTTGATGCAGTGGTGGAAGCTGTGGCACGAACATCTCTGATTCCAGA
  GTTTTCTGATGGCTTCTGGACAGGGGCCCAGCTGGCATGCTGGACAAATTCTGAAACGCCATGGGCATAT
  TTCCCTAAGATTTCTATCTACCTGAGAGATGAGAATGCCAGTCGCTCCTTCCGGATCACCATTCTCCCAC
  AGCTCTACATTCAGCCCATGATGGGAGCTGGTTTCAATTATGAATGCTACCGTTTTGGTATCTCCTCTTC
  CACAAATGCGCTGGTGATTGGTGCGACCGTGATGGAAGGCTTCTACGTGGTCTTTGACAGAGCTCAGAGG
  AGGGTGGGCTTTGCAGTGAGTCCCTGTGCAGAGATTGAAGGTACCACAGTGTCTGAAATTTCTGGGCCCT
  TTTCCACGGAAGACATAGCCAGCAACTGTGTTCCAGCACAGGCTCTGAATGAGCCCATCTTGTGGATTGT
  GTCCTATGCCCTGATGAGTGTGTGTGGAGCCATTCTCCTGGTTCTGATCCTCCTCCTGCTGCTCCCGCTG
  CACTGCCGTCATGCCCCCCGAGACCCTGAGGTAGTTAACGATGAGTCCTCACTAGTCAGACATCGCTGGA
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220631 representing NM_019517
  Red=Cloning site Green=Tags(s)
  
  MGALLRALLLPVLAQWLLSAVPALAPAPFTLPLQVARATNHRASAVPGLGTPELPRADGLALALEPVRAT
  ANFLAMVDNLQGDSGRGYYLEMLIGTPPQKVQILVDTGSSNFAVAGAPHSYIDTYFDSESSSTYHSKGFD
  VTVKYTQGSWTGFVGEDLVTIPKGFNSSFLVNIATIFESENFFLPGIKWNGILGLAYAALAKPSSSLETF
  FDSLVAQAKIPDIFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIG
  GQNLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARTSLIPEFSDGFWTGAQLACWTNSETPWAY
  FPKISIYLRDENASRSFRITILPQLYIQPMMGAGFNYECYRFGISSSTNALVIGATVMEGFYVVFDRAQR
  RVGFAVSPCAEIEGTTVSEISGPFSTEDIASNCVPAQALNEPILWIVSYALMSVCGAILLVLILLLLLPL
  HCRHAPRDPEVVNDESSLVRHRWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019517
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019517.5, NP_062390.3
• RefSeq Size: 3736 bp
• RefSeq ORF: 1545 bp
• Locus ID: 56175
• UniProt ID: Q9JL18
• Cytogenetics: 16 C4
• MW: 56.3 kDa
• Gene Summary: This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein undergoes proteolytic processing to generate an active endopeptidase enzyme. This transmembrane protease catalyzes the proteolysis of amyloid precursor protein to produce amyloid beta peptide. Mice lacking the encoded product exhibit increased pancreatic beta cell mass and improved glucose tolerance due to increased insulin secretion. [provided by RefSeq, Jul 2016]