Camsap1 (NM_001115076) Mouse Tagged ORF Clone

CAT#: MR220493

Camsap1 (Myc-DDK-tagged) - Mouse calmodulin regulated spectrin-associated protein 1 (Camsap1)

Product Images

Circular map for MR220493

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Camsap1
• Synonyms: 9530003A05Rik; C77823; PRO2405
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220493 representing NM_001115076
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACGCGGGCGGTCGCTGTGCCGCCGAAGGCTGGAGGAGGATGGAGGCGCCCCCGGAGGGCGCCG
  ACCTGGTGCCGCTGGACCGCTACGACGCGGCGCGGGCCAAGATCGCCGCCAACCTGCAGTGGATCTGTGC
  GAAGGCCTATGGCCTAGACAACATCCCTGAGGACCTCCGAGACCCGTTTTACATCGACCAGTATGAGCAG
  GAGCACATTAAGCCACCCGTTATCAAGCTTCTCCTGTCCAGTGAGCTGTATTGCCGTGTCTGCAGCCTCA
  TCCTAAAAGGGGACCAGGTGGCTACCTTGCAAGGACACCAGTCTGTCATCCAGGCCCTGTCCCGGAAGGG
  CATCTATGTGATGGAGAGTGATGATACCCCTGTGACAGATGCTGACCTCAGCCAGGCACCTATTAAGATG
  AGTGGCCACATGGCGATGGTGGATGCCCTGATGATGGCCTACACTGTGGAGATGATCAGCATTGAGAAGG
  TGGTGGCCAGTGTCAAGCGCTTTTCTACATTCAGCGCCTCAAAAGAACTTCCTTATGACCTCGAGGATGC
  CATGGTATTCTGGATCAATAAGGTAAATCTTAAAATGAGAGAGATAACAGAGAAAGAAGTTAAATTAAAA
  CAGCAGCCACTGGAAAGTCCCGCTCATCAAAAGGTCCGTTATCGGCGAGAGCACCTCTCTGCTAGGCAGT
  CACCCTACTTCCCGTTGTTGGAGGACTTGATGAGAGACGGCAGTGATGGCGCTGCTCTCTTAGCAGTGGT
  TCACTATTATTGCCCAGAGCAGATGAAACTGGATGATATCTGTCTGAAGGAGGTGCCATCAATGGCTGAC
  AGCCTCTATAACATCCGCCTTCTGAGGGAGTTTTCCAACGAGCATCTGAACAAGTGTTTCTATCTCACGC
  TGGAGGATATGCTGTATGCCCCCCTAGTGCTGAAGCCGAACGTTATGGTTTTTATCGCTGAGCTCTTCTG
  GTGGTTTGAGAACGTCAAACCAGATTTTGTTCAGCCCCGAGATATTCAAGAGCTGAAGGATGCTAAAACA
  GTGTTACAGCAGAAGAGCAGCCGGCCTCCTGTCCCTATCTCCAATGCAACCAAACGCAGCTTCCTGGGTA
  GCCCTGCTGCCATGAGCCCCGCTGACCAGCCACCATCCACCCAGCCTCTTGCTGAAGGCAGTCATCGGTA
  CCACCTCCACTCGGAAGAACCTGAGTGTCTTGGGAAAGGAGCTTCCACATTTAGTCCGTCCCATCCTTTG
  TTGCCACTGAGACAGAAACAGCAAAAAGTGTCACAGACAGAGGAAATCCCTGATCAGCGGCACAGGTCGA
  ATTCTTTAACTCGGGTTGATGGTCAGCCACGAGGTGCAATAGGAGCATGGCCAGATAAAAAAAACAGGCC
  TGTGTCACAGCCAACATCATTTGCTCTCCATCATGCTGCAAGTTGTGATGTGGATCCCAGTTCTGGTGAC
  AGTGTCAGCTTAGCTCGCTCTATCAGCAAAGATAGCTTGGCATCTAACATTATCCACCTGACCCCACAGA
  ACCAGCCCCATCCCTCCGCTGGAAAGAGCAATGGGAAAAGCCTCCTGAGCAATGTCAACATTGAGGATGA
  GGACGAGGAGCTTGTAGCCATCATCAGGACAGATGTGTCTCCTCCTTCCCCACAGATGCCCAGGACCTCA
  CCTCAGGCCCCAGGCCTGGTAGCAAGTATCAGGTCTCCCCAGAGACAGGCAGACACTTTGGAGAGTAAGC
  CTGACAGTTTTTACTTAGAACCCCTGATGCCAGCAGTACTCCGGCCAGCCAAAGAGAAGCAGATTACCAC
  TAAGGAAGATGAGCGTGGAGAAGGAAGGCCAAGGACCATCATGGCCAAGAGGCCTAGTGAGGGCTCCCAG
  CCTATGGTACGAAAGAAAGTAAGTGGTGGCCATGGCAGTCGTGACCTGAACAGAACTTTCACCCCAATTC
  CTTGTTCAGAATTTGCTGCGAGCATTGATCTTGCGGAGGTAGGACCACAGTCAGCAGAAGCTACAGGGGA
  AGGACAGCCTCTGGCCCTGGGTAGATTTGATACATTACCTCAGGGGCAGGCTGCTGATGGTTTCTTTCTC
  CATGTAGGCAGGGCTGAGGAAGATGAGGGGAGGTGGTACGTTGGTTCACAAAGTCCTAGCTCCCACGACT
  CAGAACCCTGGACCATTTTGAGGCAAGACTCGGACTCTGATGTAGTAGACGTAGAGGATACAGAGCAGGA
  TTTTATTGGTGAGGACCACCCTGTGGTTATCCCCAGATATGCTGGTGAGGAAGAGTCAGCCAAACTACAG
  GAGGATATGAAAGTGAAGGAACATGAAGACAAGGATGATGCCAGCGGCCGCTCAAGCCCGTGTCTGAGCA
  CCACTTCTCAGCTCAGCAGCATGTCCATGGCCAGTGGAAGTGTAAAGATGACCAGCTTTGCTGAAAGGAA
  GCTCCAGCGGCTCAACAGCTGTGAGACCAAGTCCAGCACCAGCAGCTCCCAGAAGACCACCCCTGATGCA
  TCTGAAAGCTGCCCAGCCCCTCTGACAACGTGGCGCCAGAAGAGGGAGCAGAGTCCAGGCAGGCACAGCA
  AGGACCCTGCCAGCCTGCTGGCTTCTGAGCTGGTGCAGCTGCACATGCAGCTGGAGGAAAAGCGCAGAGC
  TATTGAAGCCCAGAAGAAAAAGATGGAGGCGCTGTCCGCACGGCAGCGTCTGAAGCTGGGCAAGGCAGCC
  TTTCTGCATGTGGTAAAGAAAGGGAAGGCTGATGGTGCCCCACAGCCACTGAGACCCGAACACTTCACAA
  AGGAATTCACACAGCACAATGGGGAGGACTTGGATGATGGCACTTGTAAAACTGAAGGGTTCCTTGTCAA
  AGAAGAGCAGAGAGATCTCAGTGACGCTCAGGATGTGGCATTTGTCCAGCTACATAAGCCCAGGGACCCA
  GCTGCTCTGCATGATGGAGAGAAGCACAGAATGATTTCTACGGCCCTCCTCGAGGATAGTGTTGGTGAGG
  TAGATGTGAACGAGTGTGACCTGTCCATTGAGAAGCTCAATGAGACCATCAGTACATTGCAGCAGGCTAT
  ACTGAAGATCTCCCAGCAGCAAGAGCAGCTTCTCATGAAGTCCCCCACAGTGCCAACACCGGGCACTAAA
  AATAACTGCCAGGACCAAAAAATAAAGGCCCCAGTCCACTTTGTTGAGCCACTCTCTCCAACTGGCGTGC
  CTGGCCACCGCAAACCACCACGGCTTGGCCAGGGCCGGAATTCCCGCTCAGGAAGGCCGGCTGAACTGAA
  GGTTCCAAAAGACAGGCAGCAGGGTTGTTCTCGGAGCAAAACCCCAACACCCAGTGTGGAGACACTCCCA
  CAGTCAAGGTCTTTACCTCCAAGTACCCATCCACGGTCACCCTCCGACCCAGGCGGGGAACTCCCTGAGA
  AGTGTCTCTTCGACAGTTATAGGCTCCATGATGAGAGTAACCATCGGACATTTGTTCTGTCCTCTTGCAA
  AGATGCAAACATTGTATCAGAACAGGTGAACTTTAAAGAGGGTCTAGATACAAGTGTGAAAGAGGCAGGG
  CTGAGCTCCTCTACCATCACAGGCAAAGAACATACTCCAGTGGAAGAGCCGCTGAGGAGCAAGGCCAGCC
  TCATTGAGGTAGACCTCTCTGACCTGAAGGCCCCTGATGAGGATGGAGAGGTGGTTGGCCATGAGAGCTC
  GGTGGAGCTTGGTGGAGATAGTGACCAGAAGCCTGGGGTTGGTTTCTTCTTCAAGGATGAACAGAAAGCA
  GAAGATGAGCTTGCTAAGAAGCGGGCAGCCTTCCTTTTGAAACAGCAGCGCAAGGCTGAAGAGGCTCGTG
  CGCGCAAGCAGCAGCTGGAGGCAGAAGTGGAGCTCAAGCGAGATGAAGCCCGGCGTAAGGCTGAAGAGGA
  TCGACTACGGAAGGAGGAGGAGAAGGCAAGGCGAGAGCTCATTAAACAGGAATACTTACGGAGGAAGCAG
  CAGCAGGCCTTGGAGGAGCAAGGACTTGGCAAACCTAAATCAAAGCCTAAAAAGCCTCGGCCAAAGTCAG
  TTCACCGGGAAGAGTCTTACAGCGACTCTGGCACCAAGTGCTCTTCTACCCATAACTTGAGCCAAACTCA
  CTCTGGCTCCAGCCTATCCTTGGCATCTGCAGCAACAACAGAACCTGAGAGTGTTTATTCGGGGGGCACA
  CCTTCTCACCGAGTTGAATCACTGGAAGCTTTACCTATCCTGAGCCGCAACCCCAGTCGGAGCACAGACC
  GAGACTGGGAGACTGCATCAGCAGCTTCCTCTTTGGCCTCTGTGGCTGAGTACACAGGTCCTAAACTCTT
  TAAGGAGCCCAGTAGCAAATCAAACAAACCAATTATTCACAATGCCATCTCCCACTGCTGTCTGGCTGGA
  AAAGTGAATGAGCCTCACAAGAATTCAATATTGGAGCTGGAGAAGTGTGATGCCAACCACTATATTATTC
  TGTTCCGAGATGCGGGCTGCCAGTTCAGGGCACTTTACTGCTACCAACCCGACACTGAAGAAATCTACAA
  ACTCACTGGCACGGGGCCAAAAAGCATCACCAAGAAGATGATTGACAAACTATATAAATACAGCTCAGAC
  CGAAAGCAGTTTAACCTGATCCCAGCCAAGACCATGTCAGTCAGCGTGGATGCACTCACGATCCATAACC
  ACTTGTGGCAGCCCAAGCGGCCCACGGTGCCAAAGAAGACCCAGACTCGTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220493 representing NM_001115076
  Red=Cloning site Green=Tags(s)
  
  MVDAGGRCAAEGWRRMEAPPEGADLVPLDRYDAARAKIAANLQWICAKAYGLDNIPEDLRDPFYIDQYEQ
  EHIKPPVIKLLLSSELYCRVCSLILKGDQVATLQGHQSVIQALSRKGIYVMESDDTPVTDADLSQAPIKM
  SGHMAMVDALMMAYTVEMISIEKVVASVKRFSTFSASKELPYDLEDAMVFWINKVNLKMREITEKEVKLK
  QQPLESPAHQKVRYRREHLSARQSPYFPLLEDLMRDGSDGAALLAVVHYYCPEQMKLDDICLKEVPSMAD
  SLYNIRLLREFSNEHLNKCFYLTLEDMLYAPLVLKPNVMVFIAELFWWFENVKPDFVQPRDIQELKDAKT
  VLQQKSSRPPVPISNATKRSFLGSPAAMSPADQPPSTQPLAEGSHRYHLHSEEPECLGKGASTFSPSHPL
  LPLRQKQQKVSQTEEIPDQRHRSNSLTRVDGQPRGAIGAWPDKKNRPVSQPTSFALHHAASCDVDPSSGD
  SVSLARSISKDSLASNIIHLTPQNQPHPSAGKSNGKSLLSNVNIEDEDEELVAIIRTDVSPPSPQMPRTS
  PQAPGLVASIRSPQRQADTLESKPDSFYLEPLMPAVLRPAKEKQITTKEDERGEGRPRTIMAKRPSEGSQ
  PMVRKKVSGGHGSRDLNRTFTPIPCSEFAASIDLAEVGPQSAEATGEGQPLALGRFDTLPQGQAADGFFL
  HVGRAEEDEGRWYVGSQSPSSHDSEPWTILRQDSDSDVVDVEDTEQDFIGEDHPVVIPRYAGEEESAKLQ
  EDMKVKEHEDKDDASGRSSPCLSTTSQLSSMSMASGSVKMTSFAERKLQRLNSCETKSSTSSSQKTTPDA
  SESCPAPLTTWRQKREQSPGRHSKDPASLLASELVQLHMQLEEKRRAIEAQKKKMEALSARQRLKLGKAA
  FLHVVKKGKADGAPQPLRPEHFTKEFTQHNGEDLDDGTCKTEGFLVKEEQRDLSDAQDVAFVQLHKPRDP
  AALHDGEKHRMISTALLEDSVGEVDVNECDLSIEKLNETISTLQQAILKISQQQEQLLMKSPTVPTPGTK
  NNCQDQKIKAPVHFVEPLSPTGVPGHRKPPRLGQGRNSRSGRPAELKVPKDRQQGCSRSKTPTPSVETLP
  QSRSLPPSTHPRSPSDPGGELPEKCLFDSYRLHDESNHRTFVLSSCKDANIVSEQVNFKEGLDTSVKEAG
  LSSSTITGKEHTPVEEPLRSKASLIEVDLSDLKAPDEDGEVVGHESSVELGGDSDQKPGVGFFFKDEQKA
  EDELAKKRAAFLLKQQRKAEEARARKQQLEAEVELKRDEARRKAEEDRLRKEEEKARRELIKQEYLRRKQ
  QQALEEQGLGKPKSKPKKPRPKSVHREESYSDSGTKCSSTHNLSQTHSGSSLSLASAATTEPESVYSGGT
  PSHRVESLEALPILSRNPSRSTDRDWETASAASSLASVAEYTGPKLFKEPSSKSNKPIIHNAISHCCLAG
  KVNEPHKNSILELEKCDANHYIILFRDAGCQFRALYCYQPDTEEIYKLTGTGPKSITKKMIDKLYKYSSD
  RKQFNLIPAKTMSVSVDALTIHNHLWQPKRPTVPKKTQTRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001115076
• ORF Size: 4743 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001115076.1, NP_001108548.1
• RefSeq Size: 7978 bp
• RefSeq ORF: 4746 bp
• Locus ID: 227634
• Cytogenetics: 2 A3
• MW: 175.9 kDa
• Gene Summary: Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization. Specifically recognizes growing microtubule minus-ends and stabilizes microtubules. Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization. In contrast to CAMSAP2 and CAMSAP3, tracks along the growing tips of minus-end microtubules without significantly affecting the polymerization rate: binds at the very tip of the microtubules minus-end and acts as a minus-end tracking protein (-TIP) that dissociates from microtubules after allowing tubulin incorporation. Through interaction with spectrin may regulate neurite outgrowth.[UniProtKB/Swiss-Prot Function]