Eps8l1 (NM_026146) Mouse Tagged ORF Clone

CAT#: MR220159

Eps8l1 (Myc-DDK-tagged) - Mouse EPS8-like 1 (Eps8l1)

Product Images

Circular map for MR220159

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eps8l1
• Synonyms: 2310051G19Rik; 4632407K17Rik; AW060268; DRC3; EPS8R1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220159 representing NM_026146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACCACCACAGGGCCTGAAGCTGCACCCAAACCAAGTGCAAAGTCTATCTATGAACAGAGAAAAC
  GTTACTCCACCGTGGTTATGGCTGACGTGTCCCAGTATCATGTCAATCACCTGGTCACCTTCTGCTTGGG
  GGAAGAAGATGGCGTGCACACTGTGGAAGATGCTTCCAGAAAGCTGGCAGTCATGGACAGCCAGGGGCGT
  GTCTGGGCACAGGAGATGCTACTGAGAGTGTCACCTAGTCAAGTCACTCTGCTTGATCCAGTCTCCAAGG
  AAGAACTAGAGTCATATCCTCTCGATGCCATCGTACGCTGCGACGCGGTGATGCCCCGAGGACGGAGCCG
  TTCATTGCTGCTCCTGGTGTGCCAGGAGCCAGAGCGTGCACAGCCGGATGTTCATTTCTTCCAGGGCCTG
  CTCCTCGGAGCGGAGCTGATCCGTGAGGACATCCAAGGGGCTCTGCAGAACTACCGCTCCGGACGTGGAG
  AACGCAGGGCAGCAGCGCTCAGGGCGACGCAGGAGGAGCTGCGACGCGGCGCCTCTCCAGCAGCGGAGAC
  CCCGCCCTTACAGCGTCGCCCGTCTGTCCGCCTGGTGATCAACACCGTGGAACCATCCGCAGTCCGCGGG
  CGACCCCAAGTGGAGTCAATTCCAGAGACTGAGGAAGCAAGGAAACCCGACCAAGCTCGCACGACTAGCA
  GTGCTGACCCTACCTCCCCAGACTTGGGCCCAAGAGGACCTGAGCTGGCGGGTCTGCAGGCAGAGCGGGA
  TGTGGATATTCTGAACCACGTGTTTGACGACGTGGAGAGTTTTGTATCCAGACTGCAAAAATCAGCAGAG
  GCTACCCGGGTTTTGGAGCACCGCGAGAGAGGCCGCAGGACACGACGCCGAGCTGCGGGAGAGGGTCTGC
  TGACACTGAGGGCCAAGCCACCCACTGAGGCTGAGTACACCGACGTGCTTCAGAAAATCAAATACGCCTT
  CAGTCTGCTGGCCCGGTTGCGAGGCAACATCGCCAACCCCTCCTCTCCAGAGCTGTTGCACTTTCTGTTT
  GGACCTCTGCAGATGATTGTGAACACTTCAGGCGGCCCTGAGTTCGCGAAGAGTGTGCGACGACCGCATT
  TGACGTTGGAGGCTGTGACTCTGCTCCGCGACAATGTCACTCCTGGTGAAAATGAACTATGGACCTCGCT
  GGGAGACTCCTGGACCTGCCCAGGGGTGGAGCTGCCCCCGGAAGAAGGGTCTCCATACAGTCCGGAATTC
  TACAATGGCTGGGAGCCTCCGGCCACTGATCCACAGGGCCGCCCCTGGGAGGACCCAGTGGAAAAACAGC
  TACAACACGAGAAGCGGCGCAGGCAGCAAAGCGCCCCCCAGGTCGCGGTCAATGGTCAGCAAGATCCAGA
  ACTGGAAACTGAGTCCCAGCTAGAAGAGAAAGCCAGAAAATGGGTTCTGTGTAATTATGACTTCCAGGCC
  CGAAATGGCAGCGAGCTATCTGTCAAGCACCGAGATGTGTTGGAGGTCCTGGATGACAGGCGCAAATGGT
  GGAAGGTTCGGGACCATCAGGGACAAGAGGGCTATGTACCCTATAATATCCTGACACCCCACCCTGGACC
  TCAGGTGCACCGCAGCCAAAGCCCCGCAAGACACCTAGAGACTAGCACACCTCCTCCCCCACCTGCCCCA
  GCTCCAGCCCCAACTCAGGTGCGGCCCCAGTGGGACAGTTGCGACAGTCTAAACAGTTTGGATCCCAGCG
  AGAAGGAGAAATTCTCCCAGATGCTTTGTGTCAATGAGGAGCTGCAGTCGCGCCTGGCGCAGGGCCGTTC
  GGGTCCCAGCCGGGTAACCCCGGGGCCCCGAGCCCAAGAGCCCCAGCTCAGCCCGCGCTCTGAGGCCTCG
  GTGGTCCGTGCCTGGCTGCAGACCAAGGGCTTTAGCTCCGGGACTGTGGAGGCGCTCGGTGTGCTGACCG
  GCGCACAGCTCTTCTCGCTGCAAAAGGAAGAGTTGCGGGCGGTGTGCCCCGAGGAAGGGGCGCGAGTGTA
  CAGCCAAGTCACTGTGCAGCGCGCGCTGCTGGAGGACAGAGAAAAAGTATCAGAGCTGGAGGCCGTGATG
  GAGAAGCAAAAGAAAAAAGTGGAAGGCGAGACCAAAACAGAAGTCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220159 representing NM_026146
  Red=Cloning site Green=Tags(s)
  
  MSTTTGPEAAPKPSAKSIYEQRKRYSTVVMADVSQYHVNHLVTFCLGEEDGVHTVEDASRKLAVMDSQGR
  VWAQEMLLRVSPSQVTLLDPVSKEELESYPLDAIVRCDAVMPRGRSRSLLLLVCQEPERAQPDVHFFQGL
  LLGAELIREDIQGALQNYRSGRGERRAAALRATQEELRRGASPAAETPPLQRRPSVRLVINTVEPSAVRG
  RPQVESIPETEEARKPDQARTTSSADPTSPDLGPRGPELAGLQAERDVDILNHVFDDVESFVSRLQKSAE
  ATRVLEHRERGRRTRRRAAGEGLLTLRAKPPTEAEYTDVLQKIKYAFSLLARLRGNIANPSSPELLHFLF
  GPLQMIVNTSGGPEFAKSVRRPHLTLEAVTLLRDNVTPGENELWTSLGDSWTCPGVELPPEEGSPYSPEF
  YNGWEPPATDPQGRPWEDPVEKQLQHEKRRRQQSAPQVAVNGQQDPELETESQLEEKARKWVLCNYDFQA
  RNGSELSVKHRDVLEVLDDRRKWWKVRDHQGQEGYVPYNILTPHPGPQVHRSQSPARHLETSTPPPPPAP
  APAPTQVRPQWDSCDSLNSLDPSEKEKFSQMLCVNEELQSRLAQGRSGPSRVTPGPRAQEPQLSPRSEAS
  VVRAWLQTKGFSSGTVEALGVLTGAQLFSLQKEELRAVCPEEGARVYSQVTVQRALLEDREKVSELEAVM
  EKQKKKVEGETKTEVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026146
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026146.4, NP_080422.1
• RefSeq Size: 3774 bp
• RefSeq ORF: 2151 bp
• Locus ID: 67425
• UniProt ID: Q8R5F8
• Cytogenetics: 7 A1
• MW: 80 kDa
• Gene Summary: Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function]