Gbgt1 (NM_139197) Mouse Tagged ORF Clone

CAT#: MR220023

Gbgt1 (Myc-DDK-tagged) - Mouse globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 (Gbgt1)

Product Images

Circular map for MR220023

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gbgt1
• Synonyms: Fgs; Fs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220023 representing NM_139197
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCGCCCAAGACTGGCCCAGGGCCTAGCGTTCTTCCTGCTCGGTGGCACAGGTCTCTGGGTCCTGT
  GGAAGTTTATCAAGGACTGGCTGCTGGTCTCCTACATACCCTATTACCTCCCCTGCCCAGAGTTCTTCAA
  CATGAAGCTGCCCTTCAGGAAGGAGAAACCGTTGCAGCCTGTGACACAGTTACAGTACCCTCAGCCTAAG
  CTGCTGGAACACGGACCCACAGAGCTGCTGACACTTACCCCGTGGTTGGCACCCATCGTCTCCGAGGGAA
  CCTTTGACCCGGAGCTTCTGAAGAGCATGTACCAGCCGTTGAACCTGACCATTGGCGTCACCGTGTTTGC
  CGTGGGGAAGTACACCTGCTTCATTCAGCGCTTCCTGGAGTCAGCTGAAGAGTTCTTTATGCGTGGGTAC
  CAAGTACACTATTACCTCTTTACCCATGATCCTACAGCCGTTCCCAGAGTTCCCCTGGGCCCCGGTCGGC
  TCCTCAGCATCATCCCTATCCAGGGTTATTCCCGGTGGGAGGAGATCTCCATGCGCCGAATGGAGACCAT
  CAACAAACACATTGCCAAGAGAGCCCACAAAGAAGTGGATTACCTCTTCTGTGTCGATGTGGATATGGTC
  TTCCGTAACCCATGGGGCCCCGAGACCTTGGGGGATCTAGTGGCTGCCATTCACCCAGGCTATTTTGCTG
  TACCTCGCCGGAAATTCCCTTATGAACGCAGGCAAGTTTCCTCTGCCTTTGTGGCAGACAATGAGGGGGA
  CTTCTATTATGGTGGGGCACTCTTTGGGGGACGAGTGGCCAGAGTGTATGAGTTTACCCGGGCCTGCCAC
  ATGGCCATTCTGGCAGACAAAGCTAACAGCATTATGGCAGCCTGGCAGGAGGAGAGCCACCTGAACCGCC
  ACTTCATCTGGCACAAACCATCTAAGGTGCTGTCCCCAGAGTACCTCTGGGATGAAAGGAAGCCTCGGCC
  CCGAAGCCTGAAGATGATTCGATTCTCCTCAGTGAAAAAGAATGCCAACTGGCTGAGGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220023 representing NM_139197
  Red=Cloning site Green=Tags(s)
  
  MTRPRLAQGLAFFLLGGTGLWVLWKFIKDWLLVSYIPYYLPCPEFFNMKLPFRKEKPLQPVTQLQYPQPK
  LLEHGPTELLTLTPWLAPIVSEGTFDPELLKSMYQPLNLTIGVTVFAVGKYTCFIQRFLESAEEFFMRGY
  QVHYYLFTHDPTAVPRVPLGPGRLLSIIPIQGYSRWEEISMRRMETINKHIAKRAHKEVDYLFCVDVDMV
  FRNPWGPETLGDLVAAIHPGYFAVPRRKFPYERRQVSSAFVADNEGDFYYGGALFGGRVARVYEFTRACH
  MAILADKANSIMAAWQEESHLNRHFIWHKPSKVLSPEYLWDERKPRPRSLKMIRFSSVKKNANWLRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139197
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139197.2, NP_631936.2
• RefSeq Size: 1335 bp
• RefSeq ORF: 1044 bp
• Locus ID: 227671
• UniProt ID: Q8VI38
• Cytogenetics: 2 A3
• MW: 41 kDa
• Gene Summary: Catalyzes the formation of Forssman glycolipid via the addition of N-acetylgalactosamine (GalNAc) in alpha-1,3-linkage to GalNAcb-1,3Gala-1,4Galb-1,4GlcCer (Gb4Cer). Forssman glycolipid (also called Forssman antigen; FG) probably serves for adherence of some pathogens. Conversely, it diminishes Shiga toxins susceptibility.[UniProtKB/Swiss-Prot Function]